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Identification and localization of a caleosin in olive (Olea europaea L.) pollen during in vitro germination.

Zienkiewicz K, Castro AJ, Alché Jde D, Zienkiewicz A, Suárez C, Rodríguez-García MI - J. Exp. Bot. (2010)

Bottom Line: In addition, caleosins were also visualized in the cytoplasm at the subapical zone, as well as in the tonoplast of vacuoles present in the pollen tube cytoplasm.The number of oil bodies decreased 20-fold in the pollen grain during germination, whereas the opposite tendency occurred in the pollen tube, suggesting that oil bodies moved from one to the other.The data suggest that this pollen caleosin might have a role in the mobilization of oil bodies as well as in the reorganization of membrane compartments during pollen in vitro germination.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Bioquímica, Biología Celular y Molecular de Plantas, Estación Experimental del Zaidín (CSIC), Profesor Albareda 1, 18008, Granada, Spain.

ABSTRACT
In plant organs and tissues, the neutral storage lipids are confined to discrete spherical organelles called oil bodies. Oil bodies from plant seeds contain 0.6-3% proteins, including oleosins, steroleosins, and caleosins. In this study, a caleosin isoform of approximately 30 kDa was identified in the olive pollen grain. The protein was mainly located at the boundaries of the oil bodies in the cytoplasm of the pollen grain and the pollen tube. In addition, caleosins were also visualized in the cytoplasm at the subapical zone, as well as in the tonoplast of vacuoles present in the pollen tube cytoplasm. The cellular behaviour of lipid bodies in the olive pollen was also monitored during in vitro germination. The number of oil bodies decreased 20-fold in the pollen grain during germination, whereas the opposite tendency occurred in the pollen tube, suggesting that oil bodies moved from one to the other. The data suggest that this pollen caleosin might have a role in the mobilization of oil bodies as well as in the reorganization of membrane compartments during pollen in vitro germination.

Show MeSH
Purification of OBs from olive pollen and localization of caleosins. (A, B) Purified OBs stained with Sudan Black B and Nile red, respectively. (C, D) Localization of caleosins on isolated OBs using the anti-Clo3 Ab, at low (C) and high (D) magnification. Bars=2 μm.
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fig5: Purification of OBs from olive pollen and localization of caleosins. (A, B) Purified OBs stained with Sudan Black B and Nile red, respectively. (C, D) Localization of caleosins on isolated OBs using the anti-Clo3 Ab, at low (C) and high (D) magnification. Bars=2 μm.

Mentions: After purification, olive pollen OBs were visible as spherical structures with a diameter varying from 0.2 μm to 2 μm (Fig. 5A, B). Their lipidic nature was confirmed by Sudan Black B and Nile red staining. Immunolocalization experiments with anti-Clo3 Ab allowed confirmation of the presence of caleosins in these organelles (Fig. 5C, D). Caleosin labelling was found on the surface of OBs, often being visualized as ring-shaped fluorescent structures.


Identification and localization of a caleosin in olive (Olea europaea L.) pollen during in vitro germination.

Zienkiewicz K, Castro AJ, Alché Jde D, Zienkiewicz A, Suárez C, Rodríguez-García MI - J. Exp. Bot. (2010)

Purification of OBs from olive pollen and localization of caleosins. (A, B) Purified OBs stained with Sudan Black B and Nile red, respectively. (C, D) Localization of caleosins on isolated OBs using the anti-Clo3 Ab, at low (C) and high (D) magnification. Bars=2 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC2837266&req=5

fig5: Purification of OBs from olive pollen and localization of caleosins. (A, B) Purified OBs stained with Sudan Black B and Nile red, respectively. (C, D) Localization of caleosins on isolated OBs using the anti-Clo3 Ab, at low (C) and high (D) magnification. Bars=2 μm.
Mentions: After purification, olive pollen OBs were visible as spherical structures with a diameter varying from 0.2 μm to 2 μm (Fig. 5A, B). Their lipidic nature was confirmed by Sudan Black B and Nile red staining. Immunolocalization experiments with anti-Clo3 Ab allowed confirmation of the presence of caleosins in these organelles (Fig. 5C, D). Caleosin labelling was found on the surface of OBs, often being visualized as ring-shaped fluorescent structures.

Bottom Line: In addition, caleosins were also visualized in the cytoplasm at the subapical zone, as well as in the tonoplast of vacuoles present in the pollen tube cytoplasm.The number of oil bodies decreased 20-fold in the pollen grain during germination, whereas the opposite tendency occurred in the pollen tube, suggesting that oil bodies moved from one to the other.The data suggest that this pollen caleosin might have a role in the mobilization of oil bodies as well as in the reorganization of membrane compartments during pollen in vitro germination.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Bioquímica, Biología Celular y Molecular de Plantas, Estación Experimental del Zaidín (CSIC), Profesor Albareda 1, 18008, Granada, Spain.

ABSTRACT
In plant organs and tissues, the neutral storage lipids are confined to discrete spherical organelles called oil bodies. Oil bodies from plant seeds contain 0.6-3% proteins, including oleosins, steroleosins, and caleosins. In this study, a caleosin isoform of approximately 30 kDa was identified in the olive pollen grain. The protein was mainly located at the boundaries of the oil bodies in the cytoplasm of the pollen grain and the pollen tube. In addition, caleosins were also visualized in the cytoplasm at the subapical zone, as well as in the tonoplast of vacuoles present in the pollen tube cytoplasm. The cellular behaviour of lipid bodies in the olive pollen was also monitored during in vitro germination. The number of oil bodies decreased 20-fold in the pollen grain during germination, whereas the opposite tendency occurred in the pollen tube, suggesting that oil bodies moved from one to the other. The data suggest that this pollen caleosin might have a role in the mobilization of oil bodies as well as in the reorganization of membrane compartments during pollen in vitro germination.

Show MeSH