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Metabolic profiling of Arabidopsis thaliana epidermal cells.

Ebert B, Zöller D, Erban A, Fehrle I, Hartmann J, Niehl A, Kopka J, Fisahn J - J. Exp. Bot. (2010)

Bottom Line: Moreover, among these identified metabolites, 38 exhibited differential pool sizes in trichomes, basal or pavement cells.The application of an independent component analysis confirmed the cell type-specific metabolic phenotypes.Significant pool size changes between individual cells were detectable within several classes of metabolites, namely amino acids, fatty acids and alcohols, alkanes, lipids, N-compounds, organic acids and polyhydroxy acids, polyols, sugars, sugar conjugates and phenylpropanoids.

View Article: PubMed Central - PubMed

Affiliation: Max-Planck-Institute of Molecular Plant Physiology, Campus Golm, Am Mühlenberg 1, D-14476 Potsdam OT Golm, Germany.

ABSTRACT
Metabolic phenotyping at cellular resolution may be considered one of the challenges in current plant physiology. A method is described which enables the cell type-specific metabolic analysis of epidermal cell types in Arabidopsis thaliana pavement, basal, and trichome cells. To achieve the required high spatial resolution, single cell sampling using microcapillaries was combined with routine gas chromatography-time of flight-mass spectrometry (GC-TOF-MS) based metabolite profiling. The identification and relative quantification of 117 mostly primary metabolites has been demonstrated. The majority, namely 90 compounds, were accessible without analytical background correction. Analyses were performed using cell type-specific pools of 200 microsampled individual cells. Moreover, among these identified metabolites, 38 exhibited differential pool sizes in trichomes, basal or pavement cells. The application of an independent component analysis confirmed the cell type-specific metabolic phenotypes. Significant pool size changes between individual cells were detectable within several classes of metabolites, namely amino acids, fatty acids and alcohols, alkanes, lipids, N-compounds, organic acids and polyhydroxy acids, polyols, sugars, sugar conjugates and phenylpropanoids. It is demonstrated here that the combination of microsampling and GC-MS based metabolite profiling provides a method to investigate the cellular metabolism of fully differentiated plant cell types in vivo.

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Electron micrograph of epidermal cell types. Shown are all the leaf epidermal cell types analysed in this study, namely pavement cells (PC), trichomes (T), and its supporting basal cells (BC).
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fig1: Electron micrograph of epidermal cell types. Shown are all the leaf epidermal cell types analysed in this study, namely pavement cells (PC), trichomes (T), and its supporting basal cells (BC).

Mentions: Epidermal cells represent the outer barrier of the plant to its environment. These cells perform important functions that are related to gas exchange, water homeostasis, defence and protection of the plant (Gutierrez-Alcala et al., 2000; Calo et al., 2006; Nawrath, 2006; Martin and Glover, 2007). The leaf epidermis of Arabidopsis thaliana consists of several specialized cell types: trichomes, stomata, pavement, and basal cells (Fig. 1).


Metabolic profiling of Arabidopsis thaliana epidermal cells.

Ebert B, Zöller D, Erban A, Fehrle I, Hartmann J, Niehl A, Kopka J, Fisahn J - J. Exp. Bot. (2010)

Electron micrograph of epidermal cell types. Shown are all the leaf epidermal cell types analysed in this study, namely pavement cells (PC), trichomes (T), and its supporting basal cells (BC).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC2837255&req=5

fig1: Electron micrograph of epidermal cell types. Shown are all the leaf epidermal cell types analysed in this study, namely pavement cells (PC), trichomes (T), and its supporting basal cells (BC).
Mentions: Epidermal cells represent the outer barrier of the plant to its environment. These cells perform important functions that are related to gas exchange, water homeostasis, defence and protection of the plant (Gutierrez-Alcala et al., 2000; Calo et al., 2006; Nawrath, 2006; Martin and Glover, 2007). The leaf epidermis of Arabidopsis thaliana consists of several specialized cell types: trichomes, stomata, pavement, and basal cells (Fig. 1).

Bottom Line: Moreover, among these identified metabolites, 38 exhibited differential pool sizes in trichomes, basal or pavement cells.The application of an independent component analysis confirmed the cell type-specific metabolic phenotypes.Significant pool size changes between individual cells were detectable within several classes of metabolites, namely amino acids, fatty acids and alcohols, alkanes, lipids, N-compounds, organic acids and polyhydroxy acids, polyols, sugars, sugar conjugates and phenylpropanoids.

View Article: PubMed Central - PubMed

Affiliation: Max-Planck-Institute of Molecular Plant Physiology, Campus Golm, Am Mühlenberg 1, D-14476 Potsdam OT Golm, Germany.

ABSTRACT
Metabolic phenotyping at cellular resolution may be considered one of the challenges in current plant physiology. A method is described which enables the cell type-specific metabolic analysis of epidermal cell types in Arabidopsis thaliana pavement, basal, and trichome cells. To achieve the required high spatial resolution, single cell sampling using microcapillaries was combined with routine gas chromatography-time of flight-mass spectrometry (GC-TOF-MS) based metabolite profiling. The identification and relative quantification of 117 mostly primary metabolites has been demonstrated. The majority, namely 90 compounds, were accessible without analytical background correction. Analyses were performed using cell type-specific pools of 200 microsampled individual cells. Moreover, among these identified metabolites, 38 exhibited differential pool sizes in trichomes, basal or pavement cells. The application of an independent component analysis confirmed the cell type-specific metabolic phenotypes. Significant pool size changes between individual cells were detectable within several classes of metabolites, namely amino acids, fatty acids and alcohols, alkanes, lipids, N-compounds, organic acids and polyhydroxy acids, polyols, sugars, sugar conjugates and phenylpropanoids. It is demonstrated here that the combination of microsampling and GC-MS based metabolite profiling provides a method to investigate the cellular metabolism of fully differentiated plant cell types in vivo.

Show MeSH
Related in: MedlinePlus