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Stem cells in aged mammalian ovaries.

Virant-Klun I, Skutella T - Aging (Albany NY) (2010)

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynaecology, University Medical Center Ljubljana, Ljubljana, Slovenia. irma.virant@kclj.si

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immune-system-related cells (Figure 1A and 1B)... Similar cells were also found kind of parthenogenetic embryos... The investigators concluded that they had discovered small performed important experiments on the adult mouse ovary... They found that the rare population of premeiotic germ cells which expressed the Stra 8 gene and failed to develop further... elimination of the primordial follicle reserve with the cell toxin busulfan... They found that wild-type ovaries grafted into adult human ovary: firstly, the presence of stem cells in the human adult OSE and in the human mature vesicular (Graafian) confirmed in the mouse adult ovary, and secondly, the phenomenon of epithelial-mesenchymal transitions... transcription factors characteristic of embryonic stem cells... present in the OSE layer of postmenopausal women can no longer be involved in older, postmenopausal women and for the connection between

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Ovarian surface epithelium stem cells and in vitro developed                                                    oocyte-like cells in postmenopausal women. (A) Putative stem                                            cells proliferating and growing in the cluster of scraped OSE cells                                            (inverted microscope, Hoffman illumination, magnification 400x). (B)                                            Putative stem cell and erythrocyte (inverted microscope equipped with Nikon                                            Digital Sight DS-Ri1 camera, DIC-Nomarski illumination, magnification                                            6000x).  (C) Oocyte-like cell without zona pellucida with attached                                            putative stem cells possibly acting as granulosa cells (inverted                                            microscope, Hoffman illumination, magnification 200x). (D) Oocyte-like                                            cell with zona pellucida-like structure attached to the dish bottom                                            (inverted microscope, Hoffman illumination, magnification 200x). (E)                                            Blastocyst-like structure with blastocoel-like cavity at the beginning of "hatching"(inverted microscope, Hoffman illumination, magnification                                            100x). (F) Blastocyst-like structure after "hatching"                                            (inverted microscope, Hoffman illumination, magnification 100x) (images: University                                                    Medical Centre Ljubljana, 2009).
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Figure 1: Ovarian surface epithelium stem cells and in vitro developed oocyte-like cells in postmenopausal women. (A) Putative stem cells proliferating and growing in the cluster of scraped OSE cells (inverted microscope, Hoffman illumination, magnification 400x). (B) Putative stem cell and erythrocyte (inverted microscope equipped with Nikon Digital Sight DS-Ri1 camera, DIC-Nomarski illumination, magnification 6000x). (C) Oocyte-like cell without zona pellucida with attached putative stem cells possibly acting as granulosa cells (inverted microscope, Hoffman illumination, magnification 200x). (D) Oocyte-like cell with zona pellucida-like structure attached to the dish bottom (inverted microscope, Hoffman illumination, magnification 200x). (E) Blastocyst-like structure with blastocoel-like cavity at the beginning of "hatching"(inverted microscope, Hoffman illumination, magnification 100x). (F) Blastocyst-like structure after "hatching" (inverted microscope, Hoffman illumination, magnification 100x) (images: University Medical Centre Ljubljana, 2009).

Mentions: In the scraped population of cells, which consisted primarily of epithelial cells, they found small, round, yellow-colored cells with a diameter of 2 to 4 μm and small, bubble-like structures; the cells did not respond to blood or immune-system-related cells (Figure 1A and 1B). Similar cells were also found histologically in the ovarian sections of patients. These cells were immunomagnetically isolated from the remaining population of cells and expressed a number of transcription factors and surface antigens characteristic of pluripotent embryonic stem cells. In the presence of heterologous follicular fluid obtained from the institute's in vitro fertilization program, these cells developed into round oocyte-like cells (Figure 1C and 1D) expressing a number of oocyte-specific genes. Some of these cells spontaneously developed into blastocyst-like structures (Figure 1E and 1F) which could be a kind of parthenogenetic embryos [7]. The investigators concluded that they had discovered small embryonic-like stem cells comparable to embryonic-like stem cells found in other adult human tissues and organs [8,9,10,11]. Although these small embryonic-like stem cells remain to be further characterized, they might persist in adult tissues from the embryonic period of life and play a role in rejuvenation and longevity. There have also been reports of the in vitro development of embryonic stem cells into oocyte-like cells in the mouse [12,13,1415].


Stem cells in aged mammalian ovaries.

Virant-Klun I, Skutella T - Aging (Albany NY) (2010)

Ovarian surface epithelium stem cells and in vitro developed                                                    oocyte-like cells in postmenopausal women. (A) Putative stem                                            cells proliferating and growing in the cluster of scraped OSE cells                                            (inverted microscope, Hoffman illumination, magnification 400x). (B)                                            Putative stem cell and erythrocyte (inverted microscope equipped with Nikon                                            Digital Sight DS-Ri1 camera, DIC-Nomarski illumination, magnification                                            6000x).  (C) Oocyte-like cell without zona pellucida with attached                                            putative stem cells possibly acting as granulosa cells (inverted                                            microscope, Hoffman illumination, magnification 200x). (D) Oocyte-like                                            cell with zona pellucida-like structure attached to the dish bottom                                            (inverted microscope, Hoffman illumination, magnification 200x). (E)                                            Blastocyst-like structure with blastocoel-like cavity at the beginning of "hatching"(inverted microscope, Hoffman illumination, magnification                                            100x). (F) Blastocyst-like structure after "hatching"                                            (inverted microscope, Hoffman illumination, magnification 100x) (images: University                                                    Medical Centre Ljubljana, 2009).
© Copyright Policy - open-access
Related In: Results  -  Collection

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Figure 1: Ovarian surface epithelium stem cells and in vitro developed oocyte-like cells in postmenopausal women. (A) Putative stem cells proliferating and growing in the cluster of scraped OSE cells (inverted microscope, Hoffman illumination, magnification 400x). (B) Putative stem cell and erythrocyte (inverted microscope equipped with Nikon Digital Sight DS-Ri1 camera, DIC-Nomarski illumination, magnification 6000x). (C) Oocyte-like cell without zona pellucida with attached putative stem cells possibly acting as granulosa cells (inverted microscope, Hoffman illumination, magnification 200x). (D) Oocyte-like cell with zona pellucida-like structure attached to the dish bottom (inverted microscope, Hoffman illumination, magnification 200x). (E) Blastocyst-like structure with blastocoel-like cavity at the beginning of "hatching"(inverted microscope, Hoffman illumination, magnification 100x). (F) Blastocyst-like structure after "hatching" (inverted microscope, Hoffman illumination, magnification 100x) (images: University Medical Centre Ljubljana, 2009).
Mentions: In the scraped population of cells, which consisted primarily of epithelial cells, they found small, round, yellow-colored cells with a diameter of 2 to 4 μm and small, bubble-like structures; the cells did not respond to blood or immune-system-related cells (Figure 1A and 1B). Similar cells were also found histologically in the ovarian sections of patients. These cells were immunomagnetically isolated from the remaining population of cells and expressed a number of transcription factors and surface antigens characteristic of pluripotent embryonic stem cells. In the presence of heterologous follicular fluid obtained from the institute's in vitro fertilization program, these cells developed into round oocyte-like cells (Figure 1C and 1D) expressing a number of oocyte-specific genes. Some of these cells spontaneously developed into blastocyst-like structures (Figure 1E and 1F) which could be a kind of parthenogenetic embryos [7]. The investigators concluded that they had discovered small embryonic-like stem cells comparable to embryonic-like stem cells found in other adult human tissues and organs [8,9,10,11]. Although these small embryonic-like stem cells remain to be further characterized, they might persist in adult tissues from the embryonic period of life and play a role in rejuvenation and longevity. There have also been reports of the in vitro development of embryonic stem cells into oocyte-like cells in the mouse [12,13,1415].

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynaecology, University Medical Center Ljubljana, Ljubljana, Slovenia. irma.virant@kclj.si

AUTOMATICALLY GENERATED EXCERPT
Please rate it.

immune-system-related cells (Figure 1A and 1B)... Similar cells were also found kind of parthenogenetic embryos... The investigators concluded that they had discovered small performed important experiments on the adult mouse ovary... They found that the rare population of premeiotic germ cells which expressed the Stra 8 gene and failed to develop further... elimination of the primordial follicle reserve with the cell toxin busulfan... They found that wild-type ovaries grafted into adult human ovary: firstly, the presence of stem cells in the human adult OSE and in the human mature vesicular (Graafian) confirmed in the mouse adult ovary, and secondly, the phenomenon of epithelial-mesenchymal transitions... transcription factors characteristic of embryonic stem cells... present in the OSE layer of postmenopausal women can no longer be involved in older, postmenopausal women and for the connection between

Show MeSH
Related in: MedlinePlus