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Ginsan enhances humoral antibody response to orally delivered antigen.

Na HS, Lim YJ, Yun YS, Kweon MN, Lee HC - Immune Netw (2010)

Bottom Line: Ginsan increased dendritic cells in the Peyer's patch and newly migrated dendritic cells were mostly found in the subepithelial dome region.When COX inhibitors were treated, the expression of CCL3 was reduced.Ginsan effectively enhances the humoral immune response to orally delivered antigen, mediated by CCL3 via COX.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Dankook University College of Medicine, Cheonan 330-714, Korea.

ABSTRACT

Background: There have been several reports describing the capability of ginseng extracts as an adjuvant. In this study, we tested if ginsan, a polysaccharide extracted from Panax ginseng, was effective in enhancing antibody response to orally delivered Salmonella antigen.

Methods: Ginsan was treated before oral salmonella antigen administration. Salmonella specific antibody was determined by ELISA. mRNA expression was determined by RT-PCR. Cell migration was determined by confocal microscopy and flow cytometry. COX expression was detected by western blot.

Results: Ginsan treatment before oral Salmonella antigen delivery significantly increased both secretory and serum antibody production. Ginsan increased the expression of COX in the Peyer's patches. Various genes were screened and we found that CCL3 mRNA expression was increased in the Peyer's patch. Ginsan increased dendritic cells in the Peyer's patch and newly migrated dendritic cells were mostly found in the subepithelial dome region. When COX inhibitors were treated, the expression of CCL3 was reduced. COX inhibitor also antagonized both the migration of dendritic cells and the humoral immune response against oral Salmonella antigen.

Conclusion: Ginsan effectively enhances the humoral immune response to orally delivered antigen, mediated by CCL3 via COX. Ginsan may serve as a potent vaccine suppliment for oral immunization.

No MeSH data available.


Effect of ginsan and COX-1 specific inhibitor (A) or non-specific COX-inhibitor (B) on the expression of CCL3 mRNA in Peyer's patches. Total RNA was isolated and used RT-PCR can was prepared by reverse transcription. The PCR products were visualized on 2% agarose gel using ethidium bromide. CCL3 expression was antagonized by SC-560 and aspirin (ASA) treatment. (Ct, PBS; Gs, Ginsan; Sc, SC-560; ASA).
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Figure 6: Effect of ginsan and COX-1 specific inhibitor (A) or non-specific COX-inhibitor (B) on the expression of CCL3 mRNA in Peyer's patches. Total RNA was isolated and used RT-PCR can was prepared by reverse transcription. The PCR products were visualized on 2% agarose gel using ethidium bromide. CCL3 expression was antagonized by SC-560 and aspirin (ASA) treatment. (Ct, PBS; Gs, Ginsan; Sc, SC-560; ASA).

Mentions: To test the role of COX on CCL3 expression regulation, mice were treated in combination with ginsan and COX inhibitors. Semi-quantitative RT-PCR for CCL3 mRNA in the Peyer's patch was determined following various treatments. The specific COX-1 inhibitor SC-560 moderately reduced the ginsan-enhanced CCL3 expression (Fig. 6A). ASA, a nonspecific COX inhibitor, mostly reduced the CCL3 expression induced by ginsan (Fig. 6B). However, single SC-560 or ASA did not totally suppress CCL3 expression suggesting that physiological CCL3 expression may be regulated by some other mechanism.


Ginsan enhances humoral antibody response to orally delivered antigen.

Na HS, Lim YJ, Yun YS, Kweon MN, Lee HC - Immune Netw (2010)

Effect of ginsan and COX-1 specific inhibitor (A) or non-specific COX-inhibitor (B) on the expression of CCL3 mRNA in Peyer's patches. Total RNA was isolated and used RT-PCR can was prepared by reverse transcription. The PCR products were visualized on 2% agarose gel using ethidium bromide. CCL3 expression was antagonized by SC-560 and aspirin (ASA) treatment. (Ct, PBS; Gs, Ginsan; Sc, SC-560; ASA).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2837155&req=5

Figure 6: Effect of ginsan and COX-1 specific inhibitor (A) or non-specific COX-inhibitor (B) on the expression of CCL3 mRNA in Peyer's patches. Total RNA was isolated and used RT-PCR can was prepared by reverse transcription. The PCR products were visualized on 2% agarose gel using ethidium bromide. CCL3 expression was antagonized by SC-560 and aspirin (ASA) treatment. (Ct, PBS; Gs, Ginsan; Sc, SC-560; ASA).
Mentions: To test the role of COX on CCL3 expression regulation, mice were treated in combination with ginsan and COX inhibitors. Semi-quantitative RT-PCR for CCL3 mRNA in the Peyer's patch was determined following various treatments. The specific COX-1 inhibitor SC-560 moderately reduced the ginsan-enhanced CCL3 expression (Fig. 6A). ASA, a nonspecific COX inhibitor, mostly reduced the CCL3 expression induced by ginsan (Fig. 6B). However, single SC-560 or ASA did not totally suppress CCL3 expression suggesting that physiological CCL3 expression may be regulated by some other mechanism.

Bottom Line: Ginsan increased dendritic cells in the Peyer's patch and newly migrated dendritic cells were mostly found in the subepithelial dome region.When COX inhibitors were treated, the expression of CCL3 was reduced.Ginsan effectively enhances the humoral immune response to orally delivered antigen, mediated by CCL3 via COX.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Dankook University College of Medicine, Cheonan 330-714, Korea.

ABSTRACT

Background: There have been several reports describing the capability of ginseng extracts as an adjuvant. In this study, we tested if ginsan, a polysaccharide extracted from Panax ginseng, was effective in enhancing antibody response to orally delivered Salmonella antigen.

Methods: Ginsan was treated before oral salmonella antigen administration. Salmonella specific antibody was determined by ELISA. mRNA expression was determined by RT-PCR. Cell migration was determined by confocal microscopy and flow cytometry. COX expression was detected by western blot.

Results: Ginsan treatment before oral Salmonella antigen delivery significantly increased both secretory and serum antibody production. Ginsan increased the expression of COX in the Peyer's patches. Various genes were screened and we found that CCL3 mRNA expression was increased in the Peyer's patch. Ginsan increased dendritic cells in the Peyer's patch and newly migrated dendritic cells were mostly found in the subepithelial dome region. When COX inhibitors were treated, the expression of CCL3 was reduced. COX inhibitor also antagonized both the migration of dendritic cells and the humoral immune response against oral Salmonella antigen.

Conclusion: Ginsan effectively enhances the humoral immune response to orally delivered antigen, mediated by CCL3 via COX. Ginsan may serve as a potent vaccine suppliment for oral immunization.

No MeSH data available.