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Ginsan enhances humoral antibody response to orally delivered antigen.

Na HS, Lim YJ, Yun YS, Kweon MN, Lee HC - Immune Netw (2010)

Bottom Line: Ginsan increased dendritic cells in the Peyer's patch and newly migrated dendritic cells were mostly found in the subepithelial dome region.When COX inhibitors were treated, the expression of CCL3 was reduced.Ginsan effectively enhances the humoral immune response to orally delivered antigen, mediated by CCL3 via COX.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Dankook University College of Medicine, Cheonan 330-714, Korea.

ABSTRACT

Background: There have been several reports describing the capability of ginseng extracts as an adjuvant. In this study, we tested if ginsan, a polysaccharide extracted from Panax ginseng, was effective in enhancing antibody response to orally delivered Salmonella antigen.

Methods: Ginsan was treated before oral salmonella antigen administration. Salmonella specific antibody was determined by ELISA. mRNA expression was determined by RT-PCR. Cell migration was determined by confocal microscopy and flow cytometry. COX expression was detected by western blot.

Results: Ginsan treatment before oral Salmonella antigen delivery significantly increased both secretory and serum antibody production. Ginsan increased the expression of COX in the Peyer's patches. Various genes were screened and we found that CCL3 mRNA expression was increased in the Peyer's patch. Ginsan increased dendritic cells in the Peyer's patch and newly migrated dendritic cells were mostly found in the subepithelial dome region. When COX inhibitors were treated, the expression of CCL3 was reduced. COX inhibitor also antagonized both the migration of dendritic cells and the humoral immune response against oral Salmonella antigen.

Conclusion: Ginsan effectively enhances the humoral immune response to orally delivered antigen, mediated by CCL3 via COX. Ginsan may serve as a potent vaccine suppliment for oral immunization.

No MeSH data available.


Localization of CCL3 in the Peyer's patches. Frozen sections of Peyer's patches were doubly stained with antibodies against CD11c (green) in combination with anti-CCL3 (red). Panels (A) and (C) display the controls, and panels (B) and (D) display the ginsan treatment. (A, B ×600; C, D ×1,800).
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Figure 4: Localization of CCL3 in the Peyer's patches. Frozen sections of Peyer's patches were doubly stained with antibodies against CD11c (green) in combination with anti-CCL3 (red). Panels (A) and (C) display the controls, and panels (B) and (D) display the ginsan treatment. (A, B ×600; C, D ×1,800).

Mentions: To determine CCL3 expressing cells within the Peyer's patches, frozen sections were stained with anti-CCL3 in combination with CD11c. CCL3 was present within the subepithelial dome region and CCL3 expression was markedly increased following ginsan treatment (Figs. 4A~D), consistent the RT-PCR results.


Ginsan enhances humoral antibody response to orally delivered antigen.

Na HS, Lim YJ, Yun YS, Kweon MN, Lee HC - Immune Netw (2010)

Localization of CCL3 in the Peyer's patches. Frozen sections of Peyer's patches were doubly stained with antibodies against CD11c (green) in combination with anti-CCL3 (red). Panels (A) and (C) display the controls, and panels (B) and (D) display the ginsan treatment. (A, B ×600; C, D ×1,800).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2837155&req=5

Figure 4: Localization of CCL3 in the Peyer's patches. Frozen sections of Peyer's patches were doubly stained with antibodies against CD11c (green) in combination with anti-CCL3 (red). Panels (A) and (C) display the controls, and panels (B) and (D) display the ginsan treatment. (A, B ×600; C, D ×1,800).
Mentions: To determine CCL3 expressing cells within the Peyer's patches, frozen sections were stained with anti-CCL3 in combination with CD11c. CCL3 was present within the subepithelial dome region and CCL3 expression was markedly increased following ginsan treatment (Figs. 4A~D), consistent the RT-PCR results.

Bottom Line: Ginsan increased dendritic cells in the Peyer's patch and newly migrated dendritic cells were mostly found in the subepithelial dome region.When COX inhibitors were treated, the expression of CCL3 was reduced.Ginsan effectively enhances the humoral immune response to orally delivered antigen, mediated by CCL3 via COX.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Dankook University College of Medicine, Cheonan 330-714, Korea.

ABSTRACT

Background: There have been several reports describing the capability of ginseng extracts as an adjuvant. In this study, we tested if ginsan, a polysaccharide extracted from Panax ginseng, was effective in enhancing antibody response to orally delivered Salmonella antigen.

Methods: Ginsan was treated before oral salmonella antigen administration. Salmonella specific antibody was determined by ELISA. mRNA expression was determined by RT-PCR. Cell migration was determined by confocal microscopy and flow cytometry. COX expression was detected by western blot.

Results: Ginsan treatment before oral Salmonella antigen delivery significantly increased both secretory and serum antibody production. Ginsan increased the expression of COX in the Peyer's patches. Various genes were screened and we found that CCL3 mRNA expression was increased in the Peyer's patch. Ginsan increased dendritic cells in the Peyer's patch and newly migrated dendritic cells were mostly found in the subepithelial dome region. When COX inhibitors were treated, the expression of CCL3 was reduced. COX inhibitor also antagonized both the migration of dendritic cells and the humoral immune response against oral Salmonella antigen.

Conclusion: Ginsan effectively enhances the humoral immune response to orally delivered antigen, mediated by CCL3 via COX. Ginsan may serve as a potent vaccine suppliment for oral immunization.

No MeSH data available.