C-type lectin Langerin is a beta-glucan receptor on human Langerhans cells that recognizes opportunistic and pathogenic fungi.
Bottom Line: We have screened a large panel of fungi for recognition by human Langerin and, strikingly, we observed strong binding of Langerin to a variety of Candida and Saccharomyces species and Malassezia furfur, but very weak binding was observed to Cryptococcus gattii and Cryptococcus neoformans.Notably, Langerin is the primary fungal receptor on LCs, since the interaction of LCs with the different fungi was blocked by antibodies against Langerin.Langerin recognizes both mannose and beta-glucans present on fungal cell walls and our data demonstrate that Langerin is the major fungal pathogen receptor on human LCs that recognizes pathogenic and commensal fungi.
Affiliation: Center for Experimental and Molecular Medicine, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.Show MeSH
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Mentions: Next, we investigated whether a selection of this panel also interacted with cellular Langerin. Fluorescently labelled C. albicans, C. krusei, Cr. neoformans, Cr. gattii, and zymosan were incubated with a Langerin transduced cell-line, or a mock transduced cell-line. We selected the Jurkat T cell cell-line since it does not express dectin-1, which has been identified as a primary receptor for β-glucans (Fig. 4A). C. albicans, C. krusei and zymosan strongly interacted with Langerin transduced cells, which was completely inhibited by mannan, whereas no binding was observed to mock-transduced cells (Fig. 4B). In concordance with the Langerin binding ELISA, Cr. neoformans and Cr. gattii did neither interact with the Langerin transduced cells, nor with the mock-transduced cells. These data strongly suggest that Langerin recognizes Candida species but not Cryptococcus species.
Affiliation: Center for Experimental and Molecular Medicine, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.