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C-type lectin Langerin is a beta-glucan receptor on human Langerhans cells that recognizes opportunistic and pathogenic fungi.

de Jong MA, Vriend LE, Theelen B, Taylor ME, Fluitsma D, Boekhout T, Geijtenbeek TB - Mol. Immunol. (2010)

Bottom Line: We have screened a large panel of fungi for recognition by human Langerin and, strikingly, we observed strong binding of Langerin to a variety of Candida and Saccharomyces species and Malassezia furfur, but very weak binding was observed to Cryptococcus gattii and Cryptococcus neoformans.Notably, Langerin is the primary fungal receptor on LCs, since the interaction of LCs with the different fungi was blocked by antibodies against Langerin.Langerin recognizes both mannose and beta-glucans present on fungal cell walls and our data demonstrate that Langerin is the major fungal pathogen receptor on human LCs that recognizes pathogenic and commensal fungi.

View Article: PubMed Central - PubMed

Affiliation: Center for Experimental and Molecular Medicine, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.

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Candida species and zymosan interact with cellular Langerin. (A) Langerin and dectin-1 expression on Jurkat cell-line transduced with Langerin. Thin line represents isotype control, filled histogram represents specific staining. (B) Langerin expressing cell-line was incubated with different concentrations of FITC-labelled fungi and binding was measured by flow-cytometric analysis. Pre-incubation with mannan was used to determine specificity. Data represent results from at least three independent experiments. Error bars represent standard deviation of triplicates.
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fig4: Candida species and zymosan interact with cellular Langerin. (A) Langerin and dectin-1 expression on Jurkat cell-line transduced with Langerin. Thin line represents isotype control, filled histogram represents specific staining. (B) Langerin expressing cell-line was incubated with different concentrations of FITC-labelled fungi and binding was measured by flow-cytometric analysis. Pre-incubation with mannan was used to determine specificity. Data represent results from at least three independent experiments. Error bars represent standard deviation of triplicates.

Mentions: Next, we investigated whether a selection of this panel also interacted with cellular Langerin. Fluorescently labelled C. albicans, C. krusei, Cr. neoformans, Cr. gattii, and zymosan were incubated with a Langerin transduced cell-line, or a mock transduced cell-line. We selected the Jurkat T cell cell-line since it does not express dectin-1, which has been identified as a primary receptor for β-glucans (Fig. 4A). C. albicans, C. krusei and zymosan strongly interacted with Langerin transduced cells, which was completely inhibited by mannan, whereas no binding was observed to mock-transduced cells (Fig. 4B). In concordance with the Langerin binding ELISA, Cr. neoformans and Cr. gattii did neither interact with the Langerin transduced cells, nor with the mock-transduced cells. These data strongly suggest that Langerin recognizes Candida species but not Cryptococcus species.


C-type lectin Langerin is a beta-glucan receptor on human Langerhans cells that recognizes opportunistic and pathogenic fungi.

de Jong MA, Vriend LE, Theelen B, Taylor ME, Fluitsma D, Boekhout T, Geijtenbeek TB - Mol. Immunol. (2010)

Candida species and zymosan interact with cellular Langerin. (A) Langerin and dectin-1 expression on Jurkat cell-line transduced with Langerin. Thin line represents isotype control, filled histogram represents specific staining. (B) Langerin expressing cell-line was incubated with different concentrations of FITC-labelled fungi and binding was measured by flow-cytometric analysis. Pre-incubation with mannan was used to determine specificity. Data represent results from at least three independent experiments. Error bars represent standard deviation of triplicates.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2837148&req=5

fig4: Candida species and zymosan interact with cellular Langerin. (A) Langerin and dectin-1 expression on Jurkat cell-line transduced with Langerin. Thin line represents isotype control, filled histogram represents specific staining. (B) Langerin expressing cell-line was incubated with different concentrations of FITC-labelled fungi and binding was measured by flow-cytometric analysis. Pre-incubation with mannan was used to determine specificity. Data represent results from at least three independent experiments. Error bars represent standard deviation of triplicates.
Mentions: Next, we investigated whether a selection of this panel also interacted with cellular Langerin. Fluorescently labelled C. albicans, C. krusei, Cr. neoformans, Cr. gattii, and zymosan were incubated with a Langerin transduced cell-line, or a mock transduced cell-line. We selected the Jurkat T cell cell-line since it does not express dectin-1, which has been identified as a primary receptor for β-glucans (Fig. 4A). C. albicans, C. krusei and zymosan strongly interacted with Langerin transduced cells, which was completely inhibited by mannan, whereas no binding was observed to mock-transduced cells (Fig. 4B). In concordance with the Langerin binding ELISA, Cr. neoformans and Cr. gattii did neither interact with the Langerin transduced cells, nor with the mock-transduced cells. These data strongly suggest that Langerin recognizes Candida species but not Cryptococcus species.

Bottom Line: We have screened a large panel of fungi for recognition by human Langerin and, strikingly, we observed strong binding of Langerin to a variety of Candida and Saccharomyces species and Malassezia furfur, but very weak binding was observed to Cryptococcus gattii and Cryptococcus neoformans.Notably, Langerin is the primary fungal receptor on LCs, since the interaction of LCs with the different fungi was blocked by antibodies against Langerin.Langerin recognizes both mannose and beta-glucans present on fungal cell walls and our data demonstrate that Langerin is the major fungal pathogen receptor on human LCs that recognizes pathogenic and commensal fungi.

View Article: PubMed Central - PubMed

Affiliation: Center for Experimental and Molecular Medicine, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.

Show MeSH
Related in: MedlinePlus