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JAM-A is a novel surface marker for NG2-Glia in the adult mouse brain.

Stelzer S, Ebnet K, Schwamborn JC - BMC Neurosci (2010)

Bottom Line: Although these mitotic NG2-glia cells express JAM-A, the protein never shows a polarized subcellular distribution.Also non-mitotic NG2-glia cells express JAM-A in a non-polarized pattern on their surface.Our data show that JAM-A is a novel surface marker for NG2-glia cells of the adult brain.

View Article: PubMed Central - HTML - PubMed

Affiliation: Westfälische Wilhelms-Universität Münster, ZMBE, Institute for Cell Biology, Stem Cell Biology and Regeneration Group, Von-Esmarch-Str, 56, 48149 Münster, Germany.

ABSTRACT

Background: Junctional adhesion molecule-A (JAM-A) is an adhesive protein expressed in various cell types. JAM-A localizes to the tight junctions between contacting endothelial and epithelial cells, where it contributes to cell-cell adhesion and to the control of paracellular permeability.

Results: So far, the expression pattern of JAM-A has not been described in detail for the different cell types of the adult brain. Here we show that a subset of proliferating cells in the adult mouse brain express JAM-A. We further clarify that these cells belong to the lineage of NG2-glia cells. Although these mitotic NG2-glia cells express JAM-A, the protein never shows a polarized subcellular distribution. Also non-mitotic NG2-glia cells express JAM-A in a non-polarized pattern on their surface.

Conclusions: Our data show that JAM-A is a novel surface marker for NG2-glia cells of the adult brain.

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Related in: MedlinePlus

NG2-glia cells express JAM-A. Confocal images of immunostainings of vibratome sections from the corpus callosum labeled with the indicated markers (upper boxes) are shown. In (B) the cell body labeled with an arrow in (A) is shown at higher magnification. In (C) negative controls, where only one primary antibody but the two secondary antibodies anti-mouse-Alexa-568 nm (M-Alexa-568) and anti-rabbit-Alexa-488 nm (Rb-Alexa-488) have been used, are shown. Scale bars: 10 μm
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Figure 5: NG2-glia cells express JAM-A. Confocal images of immunostainings of vibratome sections from the corpus callosum labeled with the indicated markers (upper boxes) are shown. In (B) the cell body labeled with an arrow in (A) is shown at higher magnification. In (C) negative controls, where only one primary antibody but the two secondary antibodies anti-mouse-Alexa-568 nm (M-Alexa-568) and anti-rabbit-Alexa-488 nm (Rb-Alexa-488) have been used, are shown. Scale bars: 10 μm

Mentions: Beside astrocytes and oligodendrocytes the adult mammalian brain contains a third type of macroglia, the so called NG2-glia cells [12,13]. These cells are called NG2-glia cells because they express the glycoprotein NG2. Because these cells also show a multiprocessed stellate morphology as observed for JAM-A expressing cells (Figure 3) we co-stained for NG2 and JAM-A. In all brain regions investigated more than 90% (95,5% +/- 4%) of the JAM-A positive cells were also positive for NG2 (Figure 5). Additionally, nearly all NG2 positive cells were also positive for JAM-A (96,4% +/- 6%). However, NG2 is also expressed on pericytes. Therefore we used the PDGF-alpha receptor as additional NG2-glia marker [12]. Conclusively, we found a strong co-expression of JAM-A and the PDGF-alpha receptor (Figure 6). Previously it has been described that the GFAP-GFP mice used in this study show a weak expression of GFP in NG2-glia [16]. However, with our staining procedure we never found a co-staining of GFAP-GFP and NG2 or GFAP-GFP and JAM-A.


JAM-A is a novel surface marker for NG2-Glia in the adult mouse brain.

Stelzer S, Ebnet K, Schwamborn JC - BMC Neurosci (2010)

NG2-glia cells express JAM-A. Confocal images of immunostainings of vibratome sections from the corpus callosum labeled with the indicated markers (upper boxes) are shown. In (B) the cell body labeled with an arrow in (A) is shown at higher magnification. In (C) negative controls, where only one primary antibody but the two secondary antibodies anti-mouse-Alexa-568 nm (M-Alexa-568) and anti-rabbit-Alexa-488 nm (Rb-Alexa-488) have been used, are shown. Scale bars: 10 μm
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2837050&req=5

Figure 5: NG2-glia cells express JAM-A. Confocal images of immunostainings of vibratome sections from the corpus callosum labeled with the indicated markers (upper boxes) are shown. In (B) the cell body labeled with an arrow in (A) is shown at higher magnification. In (C) negative controls, where only one primary antibody but the two secondary antibodies anti-mouse-Alexa-568 nm (M-Alexa-568) and anti-rabbit-Alexa-488 nm (Rb-Alexa-488) have been used, are shown. Scale bars: 10 μm
Mentions: Beside astrocytes and oligodendrocytes the adult mammalian brain contains a third type of macroglia, the so called NG2-glia cells [12,13]. These cells are called NG2-glia cells because they express the glycoprotein NG2. Because these cells also show a multiprocessed stellate morphology as observed for JAM-A expressing cells (Figure 3) we co-stained for NG2 and JAM-A. In all brain regions investigated more than 90% (95,5% +/- 4%) of the JAM-A positive cells were also positive for NG2 (Figure 5). Additionally, nearly all NG2 positive cells were also positive for JAM-A (96,4% +/- 6%). However, NG2 is also expressed on pericytes. Therefore we used the PDGF-alpha receptor as additional NG2-glia marker [12]. Conclusively, we found a strong co-expression of JAM-A and the PDGF-alpha receptor (Figure 6). Previously it has been described that the GFAP-GFP mice used in this study show a weak expression of GFP in NG2-glia [16]. However, with our staining procedure we never found a co-staining of GFAP-GFP and NG2 or GFAP-GFP and JAM-A.

Bottom Line: Although these mitotic NG2-glia cells express JAM-A, the protein never shows a polarized subcellular distribution.Also non-mitotic NG2-glia cells express JAM-A in a non-polarized pattern on their surface.Our data show that JAM-A is a novel surface marker for NG2-glia cells of the adult brain.

View Article: PubMed Central - HTML - PubMed

Affiliation: Westfälische Wilhelms-Universität Münster, ZMBE, Institute for Cell Biology, Stem Cell Biology and Regeneration Group, Von-Esmarch-Str, 56, 48149 Münster, Germany.

ABSTRACT

Background: Junctional adhesion molecule-A (JAM-A) is an adhesive protein expressed in various cell types. JAM-A localizes to the tight junctions between contacting endothelial and epithelial cells, where it contributes to cell-cell adhesion and to the control of paracellular permeability.

Results: So far, the expression pattern of JAM-A has not been described in detail for the different cell types of the adult brain. Here we show that a subset of proliferating cells in the adult mouse brain express JAM-A. We further clarify that these cells belong to the lineage of NG2-glia cells. Although these mitotic NG2-glia cells express JAM-A, the protein never shows a polarized subcellular distribution. Also non-mitotic NG2-glia cells express JAM-A in a non-polarized pattern on their surface.

Conclusions: Our data show that JAM-A is a novel surface marker for NG2-glia cells of the adult brain.

Show MeSH
Related in: MedlinePlus