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JAM-A is a novel surface marker for NG2-Glia in the adult mouse brain.

Stelzer S, Ebnet K, Schwamborn JC - BMC Neurosci (2010)

Bottom Line: Although these mitotic NG2-glia cells express JAM-A, the protein never shows a polarized subcellular distribution.Also non-mitotic NG2-glia cells express JAM-A in a non-polarized pattern on their surface.Our data show that JAM-A is a novel surface marker for NG2-glia cells of the adult brain.

View Article: PubMed Central - HTML - PubMed

Affiliation: Westfälische Wilhelms-Universität Münster, ZMBE, Institute for Cell Biology, Stem Cell Biology and Regeneration Group, Von-Esmarch-Str, 56, 48149 Münster, Germany.

ABSTRACT

Background: Junctional adhesion molecule-A (JAM-A) is an adhesive protein expressed in various cell types. JAM-A localizes to the tight junctions between contacting endothelial and epithelial cells, where it contributes to cell-cell adhesion and to the control of paracellular permeability.

Results: So far, the expression pattern of JAM-A has not been described in detail for the different cell types of the adult brain. Here we show that a subset of proliferating cells in the adult mouse brain express JAM-A. We further clarify that these cells belong to the lineage of NG2-glia cells. Although these mitotic NG2-glia cells express JAM-A, the protein never shows a polarized subcellular distribution. Also non-mitotic NG2-glia cells express JAM-A in a non-polarized pattern on their surface.

Conclusions: Our data show that JAM-A is a novel surface marker for NG2-glia cells of the adult brain.

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Related in: MedlinePlus

JAM-A is expressed in a subset of proliferating cells. Confocal images of immunostainings of vibratome sections from the subventricular zone of adult mouse brains labeled with the indicated markers (left boxes) are shown. (A) and (B) show proliferating P-H3 positive cells in two different magnifications. In (B) a maximum intensity projection of several optical layers is shown, the arrow points to a P-H3 positive and JAM-A negative cell, while the arrowhead indicates a P-H3 and JAM-A double positive cell. Scale bars: 10 μm
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Figure 1: JAM-A is expressed in a subset of proliferating cells. Confocal images of immunostainings of vibratome sections from the subventricular zone of adult mouse brains labeled with the indicated markers (left boxes) are shown. (A) and (B) show proliferating P-H3 positive cells in two different magnifications. In (B) a maximum intensity projection of several optical layers is shown, the arrow points to a P-H3 positive and JAM-A negative cell, while the arrowhead indicates a P-H3 and JAM-A double positive cell. Scale bars: 10 μm

Mentions: We identified mitotic cells in the SVZ by staining with an antibody against phosphorylated Histon H3 (P-H3). To detect JAM-A we used an anti-JAM-A antibody that is specific for just JAM-A and is not detecting other JAM-proteins like JAM-B or JAM-C [7]. Most P-H3 positive cells in the SVZ were negative for JAM-A. Interestingly, about 5% of the P-H3 positive cells were also positive for JAM-A (Figure 1). Analysis at higher magnification indicated that JAM-A is evenly distributed on the cell with no obvious asymmetric subcellular distribution (Figure 1B).


JAM-A is a novel surface marker for NG2-Glia in the adult mouse brain.

Stelzer S, Ebnet K, Schwamborn JC - BMC Neurosci (2010)

JAM-A is expressed in a subset of proliferating cells. Confocal images of immunostainings of vibratome sections from the subventricular zone of adult mouse brains labeled with the indicated markers (left boxes) are shown. (A) and (B) show proliferating P-H3 positive cells in two different magnifications. In (B) a maximum intensity projection of several optical layers is shown, the arrow points to a P-H3 positive and JAM-A negative cell, while the arrowhead indicates a P-H3 and JAM-A double positive cell. Scale bars: 10 μm
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2837050&req=5

Figure 1: JAM-A is expressed in a subset of proliferating cells. Confocal images of immunostainings of vibratome sections from the subventricular zone of adult mouse brains labeled with the indicated markers (left boxes) are shown. (A) and (B) show proliferating P-H3 positive cells in two different magnifications. In (B) a maximum intensity projection of several optical layers is shown, the arrow points to a P-H3 positive and JAM-A negative cell, while the arrowhead indicates a P-H3 and JAM-A double positive cell. Scale bars: 10 μm
Mentions: We identified mitotic cells in the SVZ by staining with an antibody against phosphorylated Histon H3 (P-H3). To detect JAM-A we used an anti-JAM-A antibody that is specific for just JAM-A and is not detecting other JAM-proteins like JAM-B or JAM-C [7]. Most P-H3 positive cells in the SVZ were negative for JAM-A. Interestingly, about 5% of the P-H3 positive cells were also positive for JAM-A (Figure 1). Analysis at higher magnification indicated that JAM-A is evenly distributed on the cell with no obvious asymmetric subcellular distribution (Figure 1B).

Bottom Line: Although these mitotic NG2-glia cells express JAM-A, the protein never shows a polarized subcellular distribution.Also non-mitotic NG2-glia cells express JAM-A in a non-polarized pattern on their surface.Our data show that JAM-A is a novel surface marker for NG2-glia cells of the adult brain.

View Article: PubMed Central - HTML - PubMed

Affiliation: Westfälische Wilhelms-Universität Münster, ZMBE, Institute for Cell Biology, Stem Cell Biology and Regeneration Group, Von-Esmarch-Str, 56, 48149 Münster, Germany.

ABSTRACT

Background: Junctional adhesion molecule-A (JAM-A) is an adhesive protein expressed in various cell types. JAM-A localizes to the tight junctions between contacting endothelial and epithelial cells, where it contributes to cell-cell adhesion and to the control of paracellular permeability.

Results: So far, the expression pattern of JAM-A has not been described in detail for the different cell types of the adult brain. Here we show that a subset of proliferating cells in the adult mouse brain express JAM-A. We further clarify that these cells belong to the lineage of NG2-glia cells. Although these mitotic NG2-glia cells express JAM-A, the protein never shows a polarized subcellular distribution. Also non-mitotic NG2-glia cells express JAM-A in a non-polarized pattern on their surface.

Conclusions: Our data show that JAM-A is a novel surface marker for NG2-glia cells of the adult brain.

Show MeSH
Related in: MedlinePlus