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Globular adiponectin as a complete mesoangioblast regulator: role in proliferation, survival, motility, and skeletal muscle differentiation.

Fiaschi T, Tedesco FS, Giannoni E, Diaz-Manera J, Parri M, Cossu G, Chiarugi P - Mol. Biol. Cell (2010)

Bottom Line: Mesoangioblasts are progenitor endowed with multipotent mesoderm differentiation ability.The adipokine drives mesoangioblasts to entry cell cycle and strongly counteracts the apoptotic process triggered by growth factor withdrawal, thereby serving as an activating and prosurvival stem cell factor.We conclude that adiponectin exerts several advantageous effects on mesoangioblasts, potentially valuable to improve their efficacy in cell based therapies of diseased muscles.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemical Science, University of Florence, 50134 Florence, Italy.

ABSTRACT
Mesoangioblasts are progenitor endowed with multipotent mesoderm differentiation ability. Despite the promising results obtained with mesoangioblast transplantation in muscle dystrophy, an improvement of their efficient engrafting and survival within damaged muscles, as well as their ex vivo activation/expansion and commitment toward myogenic lineage, is highly needed and should greatly increase their therapeutic potential. We show that globular adiponectin, an adipokine endowed with metabolic and differentiating functions for muscles, regulates vital cues of mesoangioblast cell biology. The adipokine drives mesoangioblasts to entry cell cycle and strongly counteracts the apoptotic process triggered by growth factor withdrawal, thereby serving as an activating and prosurvival stem cell factor. In addition, adiponectin provides a specific protection against anoikis, the apoptotic death due to lack of anchorage to extracellular matrix, suggesting a key protective role for these nonresident stem cells after systemic injection. Finally, adiponectin behaves as a chemoattractive factor toward mature myotubes and stimulates their differentiation toward the skeletal muscle lineage, serving as a positive regulator in mesoangioblast homing to injured or diseased muscles. We conclude that adiponectin exerts several advantageous effects on mesoangioblasts, potentially valuable to improve their efficacy in cell based therapies of diseased muscles.

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Related in: MedlinePlus

Expression of adiponectin receptors AdipoR1 and AdipoR2 in mesoangioblasts. (A) Amount of AdipoR1 and AdipoR2 mRNA by real-time PCR. Total RNA was used for the amplification of mRNA of adiponectin receptors using as housekeeping gene 18S rRNA. The amount of target, normalized to the endogenous reference (18S RNA), was given by the 2−ΔΔCT calculation and was reported as arbitrary units (a.u.). (B) Analysis of AdipoR1 and AdipoR2 expression by immunoblot. An equal amount of total proteins were run in each lane after protein assay with Bradford method, as shown by actin immunoblot. Real-time PCR is the mean of three independent assays, whereas the blot is representative of three different experiments. n.r., nonrelated band.
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Figure 1: Expression of adiponectin receptors AdipoR1 and AdipoR2 in mesoangioblasts. (A) Amount of AdipoR1 and AdipoR2 mRNA by real-time PCR. Total RNA was used for the amplification of mRNA of adiponectin receptors using as housekeeping gene 18S rRNA. The amount of target, normalized to the endogenous reference (18S RNA), was given by the 2−ΔΔCT calculation and was reported as arbitrary units (a.u.). (B) Analysis of AdipoR1 and AdipoR2 expression by immunoblot. An equal amount of total proteins were run in each lane after protein assay with Bradford method, as shown by actin immunoblot. Real-time PCR is the mean of three independent assays, whereas the blot is representative of three different experiments. n.r., nonrelated band.

Mentions: We initially examined whether mesoangioblasts express receptors for adiponectin. mRNA analysis by real-time PCR revealed that mesoangioblasts express both AdipoR1 and AdipoR2 messenger and protein as confirmed by immunoblot analysis (Figure 1, A and B).


Globular adiponectin as a complete mesoangioblast regulator: role in proliferation, survival, motility, and skeletal muscle differentiation.

Fiaschi T, Tedesco FS, Giannoni E, Diaz-Manera J, Parri M, Cossu G, Chiarugi P - Mol. Biol. Cell (2010)

Expression of adiponectin receptors AdipoR1 and AdipoR2 in mesoangioblasts. (A) Amount of AdipoR1 and AdipoR2 mRNA by real-time PCR. Total RNA was used for the amplification of mRNA of adiponectin receptors using as housekeeping gene 18S rRNA. The amount of target, normalized to the endogenous reference (18S RNA), was given by the 2−ΔΔCT calculation and was reported as arbitrary units (a.u.). (B) Analysis of AdipoR1 and AdipoR2 expression by immunoblot. An equal amount of total proteins were run in each lane after protein assay with Bradford method, as shown by actin immunoblot. Real-time PCR is the mean of three independent assays, whereas the blot is representative of three different experiments. n.r., nonrelated band.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2836966&req=5

Figure 1: Expression of adiponectin receptors AdipoR1 and AdipoR2 in mesoangioblasts. (A) Amount of AdipoR1 and AdipoR2 mRNA by real-time PCR. Total RNA was used for the amplification of mRNA of adiponectin receptors using as housekeeping gene 18S rRNA. The amount of target, normalized to the endogenous reference (18S RNA), was given by the 2−ΔΔCT calculation and was reported as arbitrary units (a.u.). (B) Analysis of AdipoR1 and AdipoR2 expression by immunoblot. An equal amount of total proteins were run in each lane after protein assay with Bradford method, as shown by actin immunoblot. Real-time PCR is the mean of three independent assays, whereas the blot is representative of three different experiments. n.r., nonrelated band.
Mentions: We initially examined whether mesoangioblasts express receptors for adiponectin. mRNA analysis by real-time PCR revealed that mesoangioblasts express both AdipoR1 and AdipoR2 messenger and protein as confirmed by immunoblot analysis (Figure 1, A and B).

Bottom Line: Mesoangioblasts are progenitor endowed with multipotent mesoderm differentiation ability.The adipokine drives mesoangioblasts to entry cell cycle and strongly counteracts the apoptotic process triggered by growth factor withdrawal, thereby serving as an activating and prosurvival stem cell factor.We conclude that adiponectin exerts several advantageous effects on mesoangioblasts, potentially valuable to improve their efficacy in cell based therapies of diseased muscles.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemical Science, University of Florence, 50134 Florence, Italy.

ABSTRACT
Mesoangioblasts are progenitor endowed with multipotent mesoderm differentiation ability. Despite the promising results obtained with mesoangioblast transplantation in muscle dystrophy, an improvement of their efficient engrafting and survival within damaged muscles, as well as their ex vivo activation/expansion and commitment toward myogenic lineage, is highly needed and should greatly increase their therapeutic potential. We show that globular adiponectin, an adipokine endowed with metabolic and differentiating functions for muscles, regulates vital cues of mesoangioblast cell biology. The adipokine drives mesoangioblasts to entry cell cycle and strongly counteracts the apoptotic process triggered by growth factor withdrawal, thereby serving as an activating and prosurvival stem cell factor. In addition, adiponectin provides a specific protection against anoikis, the apoptotic death due to lack of anchorage to extracellular matrix, suggesting a key protective role for these nonresident stem cells after systemic injection. Finally, adiponectin behaves as a chemoattractive factor toward mature myotubes and stimulates their differentiation toward the skeletal muscle lineage, serving as a positive regulator in mesoangioblast homing to injured or diseased muscles. We conclude that adiponectin exerts several advantageous effects on mesoangioblasts, potentially valuable to improve their efficacy in cell based therapies of diseased muscles.

Show MeSH
Related in: MedlinePlus