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Genotypic Diversity of Streptococcus mutans in Caries-Free and Caries-Active Preschool Children.

Pieralisi FJ, Rodrigues MR, Segura VG, Maciel SM, Ferreira FB, Garcia JE, Poli-Frederico RC - Int J Dent (2009)

Bottom Line: There were more S. mutans genotypes in the group of preschool children with dental caries, compared with the caries-free group.Conclusion.Our results support the previous findings of genetic diversity of S. mutans in preschool children being associated with dental caries.

View Article: PubMed Central - PubMed

Affiliation: School of Dentistry, University North of Parana, Londrina, 86041-100 ParanĂ¡, Brazil.

ABSTRACT
Aim. The aim of the present paper was to evaluate the genotypic diversity of S. mutans in caries-free and caries-active preschool children in Brazil. Design. Twenty-eight preschool children were examined regarding caries experience by the dmft index. DNA from 280 isolates of S. mutans was extracted. S. mutans evaluated using to the PCR method, with primers for the glucosyltransferase gene. The genetic diversity of S. mutans isolates was analyzed by arbitrary primed-PCR (AP-PCR) reactions. The differences between the diversity genotypic and dmft/caries experience were evaluated by chi2 test and Spearman's correlation. Results. The Spearman correlation test showed a strong association between genotypic diversity and caries experience (r = 0.72; P < .001). There were more S. mutans genotypes in the group of preschool children with dental caries, compared with the caries-free group. Among the children with more than 1 genotype, 13 had dental caries (2 to 5 genotypes) and 4 were caries-free (only 2 genotypes). Conclusion. Our results support the previous findings of genetic diversity of S. mutans in preschool children being associated with dental caries. The investigation of such populations may be important for directing the development of programs for caries prevention worldwide.

No MeSH data available.


Related in: MedlinePlus

Dendrogram illustrating genotypic diversity between S. mutans strains isolated from caries-free and caries children. The Dice coefficient was generated from UPGMA clustering analysis based upon the comparison of the similarity matrices of all S. mutans strains type. The arrows indicate the preschool children no. 2c, who showed five distinct genotypes.
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fig2: Dendrogram illustrating genotypic diversity between S. mutans strains isolated from caries-free and caries children. The Dice coefficient was generated from UPGMA clustering analysis based upon the comparison of the similarity matrices of all S. mutans strains type. The arrows indicate the preschool children no. 2c, who showed five distinct genotypes.

Mentions: Considering the whole population, some of the preschool children harbored just one genotype whereas others exhibited until five genotypes (Figure 2).


Genotypic Diversity of Streptococcus mutans in Caries-Free and Caries-Active Preschool Children.

Pieralisi FJ, Rodrigues MR, Segura VG, Maciel SM, Ferreira FB, Garcia JE, Poli-Frederico RC - Int J Dent (2009)

Dendrogram illustrating genotypic diversity between S. mutans strains isolated from caries-free and caries children. The Dice coefficient was generated from UPGMA clustering analysis based upon the comparison of the similarity matrices of all S. mutans strains type. The arrows indicate the preschool children no. 2c, who showed five distinct genotypes.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2836815&req=5

fig2: Dendrogram illustrating genotypic diversity between S. mutans strains isolated from caries-free and caries children. The Dice coefficient was generated from UPGMA clustering analysis based upon the comparison of the similarity matrices of all S. mutans strains type. The arrows indicate the preschool children no. 2c, who showed five distinct genotypes.
Mentions: Considering the whole population, some of the preschool children harbored just one genotype whereas others exhibited until five genotypes (Figure 2).

Bottom Line: There were more S. mutans genotypes in the group of preschool children with dental caries, compared with the caries-free group.Conclusion.Our results support the previous findings of genetic diversity of S. mutans in preschool children being associated with dental caries.

View Article: PubMed Central - PubMed

Affiliation: School of Dentistry, University North of Parana, Londrina, 86041-100 ParanĂ¡, Brazil.

ABSTRACT
Aim. The aim of the present paper was to evaluate the genotypic diversity of S. mutans in caries-free and caries-active preschool children in Brazil. Design. Twenty-eight preschool children were examined regarding caries experience by the dmft index. DNA from 280 isolates of S. mutans was extracted. S. mutans evaluated using to the PCR method, with primers for the glucosyltransferase gene. The genetic diversity of S. mutans isolates was analyzed by arbitrary primed-PCR (AP-PCR) reactions. The differences between the diversity genotypic and dmft/caries experience were evaluated by chi2 test and Spearman's correlation. Results. The Spearman correlation test showed a strong association between genotypic diversity and caries experience (r = 0.72; P < .001). There were more S. mutans genotypes in the group of preschool children with dental caries, compared with the caries-free group. Among the children with more than 1 genotype, 13 had dental caries (2 to 5 genotypes) and 4 were caries-free (only 2 genotypes). Conclusion. Our results support the previous findings of genetic diversity of S. mutans in preschool children being associated with dental caries. The investigation of such populations may be important for directing the development of programs for caries prevention worldwide.

No MeSH data available.


Related in: MedlinePlus