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Identification of tomato plant as a novel host model for Burkholderia pseudomallei.

Lee YH, Chen Y, Ouyang X, Gan YH - BMC Microbiol. (2010)

Bottom Line: This shows the importance of both T3SS1 and T3SS2 in bacterial pathogenesis in susceptible plants.The potential of B. pseudomallei as a plant pathogen raises new possibilities of exploiting plant as an alternative host for novel anti-infectives or virulence factor discovery.It also raises issues of biosecurity due to its classification as a potential bioterrorism agent.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, 8 Medical Drive, 117597, Singapore.

ABSTRACT

Background: Burkholderia pseudomallei is the causative agent for melioidosis, a disease with significant mortality and morbidity in endemic regions. Its versatility as a pathogen is reflected in its relatively huge 7.24 Mb genome and the presence of many virulence factors including three Type Three Secretion Systems known as T3SS1, T3SS2 and T3SS3. Besides being a human pathogen, it is able to infect and cause disease in many different animals and alternative hosts such as C. elegans.

Results: Its host range is further extended to include plants as we demonstrated the ability of B. pseudomallei and the closely related species B. thailandensis to infect susceptible tomato but not rice plants. Bacteria were found to multiply intercellularly and were found in the xylem vessels of the vascular bundle. Disease is substantially attenuated upon infection with bacterial mutants deficient in T3SS1 or T3SS2 and slightly attenuated upon infection with the T3SS3 mutant. This shows the importance of both T3SS1 and T3SS2 in bacterial pathogenesis in susceptible plants.

Conclusions: The potential of B. pseudomallei as a plant pathogen raises new possibilities of exploiting plant as an alternative host for novel anti-infectives or virulence factor discovery. It also raises issues of biosecurity due to its classification as a potential bioterrorism agent.

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B. pseudomallei and B. thailandensis infection of rice (A) and Arabidopsis (B) plantlets. Each graph represents an experiment of 6 plantlets infected either with B. pseudomallei or B. thailandensis as both types of infections resulted in identical disease scores. Each experiment with B. pseudomallei or B. thailandensis infection had been repeated twice.
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Figure 5: B. pseudomallei and B. thailandensis infection of rice (A) and Arabidopsis (B) plantlets. Each graph represents an experiment of 6 plantlets infected either with B. pseudomallei or B. thailandensis as both types of infections resulted in identical disease scores. Each experiment with B. pseudomallei or B. thailandensis infection had been repeated twice.

Mentions: Both B. thailandensis and B. pseudomallei did not cause any discernible symptoms in rice plantlets when infected via roots (unwounded or wounded) nor via inoculation through the leaves. B. thailandensis and B. pseudomallei infection of rice plantlets showed identical disease scores over 7 days (Fig 5A). We were unable to recover any bacteria from the leaves after infection via the roots. When bacteria were inoculated directly into the leaf blade, no bacteria were recoverable from the leaf one day after inoculation, indicating a lack of establishment of infection. The inoculated leaves did not show any yellowing (data not shown) as seen in the tomato leaves. Thus, rice plants are non-hosts to the bacteria. As Arabidopsis thaliana has been used extensively as a plant host model for several pathogens, we tested B. thailandensis and B. pseudomallei infection in Arabidopsis plantlets via the roots. The average disease scores were maintained at 1 and increased only slightly at days 6 and 7 and were identical for both B. thailandensis and B. pseudomallei infection (Fig 5B).


Identification of tomato plant as a novel host model for Burkholderia pseudomallei.

Lee YH, Chen Y, Ouyang X, Gan YH - BMC Microbiol. (2010)

B. pseudomallei and B. thailandensis infection of rice (A) and Arabidopsis (B) plantlets. Each graph represents an experiment of 6 plantlets infected either with B. pseudomallei or B. thailandensis as both types of infections resulted in identical disease scores. Each experiment with B. pseudomallei or B. thailandensis infection had been repeated twice.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2823722&req=5

Figure 5: B. pseudomallei and B. thailandensis infection of rice (A) and Arabidopsis (B) plantlets. Each graph represents an experiment of 6 plantlets infected either with B. pseudomallei or B. thailandensis as both types of infections resulted in identical disease scores. Each experiment with B. pseudomallei or B. thailandensis infection had been repeated twice.
Mentions: Both B. thailandensis and B. pseudomallei did not cause any discernible symptoms in rice plantlets when infected via roots (unwounded or wounded) nor via inoculation through the leaves. B. thailandensis and B. pseudomallei infection of rice plantlets showed identical disease scores over 7 days (Fig 5A). We were unable to recover any bacteria from the leaves after infection via the roots. When bacteria were inoculated directly into the leaf blade, no bacteria were recoverable from the leaf one day after inoculation, indicating a lack of establishment of infection. The inoculated leaves did not show any yellowing (data not shown) as seen in the tomato leaves. Thus, rice plants are non-hosts to the bacteria. As Arabidopsis thaliana has been used extensively as a plant host model for several pathogens, we tested B. thailandensis and B. pseudomallei infection in Arabidopsis plantlets via the roots. The average disease scores were maintained at 1 and increased only slightly at days 6 and 7 and were identical for both B. thailandensis and B. pseudomallei infection (Fig 5B).

Bottom Line: This shows the importance of both T3SS1 and T3SS2 in bacterial pathogenesis in susceptible plants.The potential of B. pseudomallei as a plant pathogen raises new possibilities of exploiting plant as an alternative host for novel anti-infectives or virulence factor discovery.It also raises issues of biosecurity due to its classification as a potential bioterrorism agent.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, 8 Medical Drive, 117597, Singapore.

ABSTRACT

Background: Burkholderia pseudomallei is the causative agent for melioidosis, a disease with significant mortality and morbidity in endemic regions. Its versatility as a pathogen is reflected in its relatively huge 7.24 Mb genome and the presence of many virulence factors including three Type Three Secretion Systems known as T3SS1, T3SS2 and T3SS3. Besides being a human pathogen, it is able to infect and cause disease in many different animals and alternative hosts such as C. elegans.

Results: Its host range is further extended to include plants as we demonstrated the ability of B. pseudomallei and the closely related species B. thailandensis to infect susceptible tomato but not rice plants. Bacteria were found to multiply intercellularly and were found in the xylem vessels of the vascular bundle. Disease is substantially attenuated upon infection with bacterial mutants deficient in T3SS1 or T3SS2 and slightly attenuated upon infection with the T3SS3 mutant. This shows the importance of both T3SS1 and T3SS2 in bacterial pathogenesis in susceptible plants.

Conclusions: The potential of B. pseudomallei as a plant pathogen raises new possibilities of exploiting plant as an alternative host for novel anti-infectives or virulence factor discovery. It also raises issues of biosecurity due to its classification as a potential bioterrorism agent.

Show MeSH
Related in: MedlinePlus