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Identification of tomato plant as a novel host model for Burkholderia pseudomallei.

Lee YH, Chen Y, Ouyang X, Gan YH - BMC Microbiol. (2010)

Bottom Line: This shows the importance of both T3SS1 and T3SS2 in bacterial pathogenesis in susceptible plants.The potential of B. pseudomallei as a plant pathogen raises new possibilities of exploiting plant as an alternative host for novel anti-infectives or virulence factor discovery.It also raises issues of biosecurity due to its classification as a potential bioterrorism agent.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, 8 Medical Drive, 117597, Singapore.

ABSTRACT

Background: Burkholderia pseudomallei is the causative agent for melioidosis, a disease with significant mortality and morbidity in endemic regions. Its versatility as a pathogen is reflected in its relatively huge 7.24 Mb genome and the presence of many virulence factors including three Type Three Secretion Systems known as T3SS1, T3SS2 and T3SS3. Besides being a human pathogen, it is able to infect and cause disease in many different animals and alternative hosts such as C. elegans.

Results: Its host range is further extended to include plants as we demonstrated the ability of B. pseudomallei and the closely related species B. thailandensis to infect susceptible tomato but not rice plants. Bacteria were found to multiply intercellularly and were found in the xylem vessels of the vascular bundle. Disease is substantially attenuated upon infection with bacterial mutants deficient in T3SS1 or T3SS2 and slightly attenuated upon infection with the T3SS3 mutant. This shows the importance of both T3SS1 and T3SS2 in bacterial pathogenesis in susceptible plants.

Conclusions: The potential of B. pseudomallei as a plant pathogen raises new possibilities of exploiting plant as an alternative host for novel anti-infectives or virulence factor discovery. It also raises issues of biosecurity due to its classification as a potential bioterrorism agent.

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Related in: MedlinePlus

Infection of tomato plantlets with different B. pseudomallei isolates. KHW and K9 (K96243) are clinical isolates, 77/96 and 109/96 are soil isolates, 561 is isolated from a kangaroo, 612 from a crown pigeon and 490 from a Bird of Paradise. The average disease score was calculated based on 12 plantlets per bacterial isolate cumulative from two experiments.
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Figure 2: Infection of tomato plantlets with different B. pseudomallei isolates. KHW and K9 (K96243) are clinical isolates, 77/96 and 109/96 are soil isolates, 561 is isolated from a kangaroo, 612 from a crown pigeon and 490 from a Bird of Paradise. The average disease score was calculated based on 12 plantlets per bacterial isolate cumulative from two experiments.

Mentions: Having established that B. thailandensis can infect tomato plantlets and cause disease, we determine whether B. pseudomallei would similarly infect tomato plantlets. We included strains isolated from humans, animals or the environment such as two clinical isolates (K96243 and KHW), a kangaroo isolate 561, two bird isolates (612 and 490) and two soil isolates (77/96 and 109/96) on their ability to infect tomato plants. B. pseudomallei is able to infect tomato plantlets to a similar degree as B. thailandensis with almost identical disease symptoms. All isolates were able to infect and cause disease to a similar extent (Fig 2), showing that the ability to infect susceptible plants is unlikely to be strain-specific.


Identification of tomato plant as a novel host model for Burkholderia pseudomallei.

Lee YH, Chen Y, Ouyang X, Gan YH - BMC Microbiol. (2010)

Infection of tomato plantlets with different B. pseudomallei isolates. KHW and K9 (K96243) are clinical isolates, 77/96 and 109/96 are soil isolates, 561 is isolated from a kangaroo, 612 from a crown pigeon and 490 from a Bird of Paradise. The average disease score was calculated based on 12 plantlets per bacterial isolate cumulative from two experiments.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2823722&req=5

Figure 2: Infection of tomato plantlets with different B. pseudomallei isolates. KHW and K9 (K96243) are clinical isolates, 77/96 and 109/96 are soil isolates, 561 is isolated from a kangaroo, 612 from a crown pigeon and 490 from a Bird of Paradise. The average disease score was calculated based on 12 plantlets per bacterial isolate cumulative from two experiments.
Mentions: Having established that B. thailandensis can infect tomato plantlets and cause disease, we determine whether B. pseudomallei would similarly infect tomato plantlets. We included strains isolated from humans, animals or the environment such as two clinical isolates (K96243 and KHW), a kangaroo isolate 561, two bird isolates (612 and 490) and two soil isolates (77/96 and 109/96) on their ability to infect tomato plants. B. pseudomallei is able to infect tomato plantlets to a similar degree as B. thailandensis with almost identical disease symptoms. All isolates were able to infect and cause disease to a similar extent (Fig 2), showing that the ability to infect susceptible plants is unlikely to be strain-specific.

Bottom Line: This shows the importance of both T3SS1 and T3SS2 in bacterial pathogenesis in susceptible plants.The potential of B. pseudomallei as a plant pathogen raises new possibilities of exploiting plant as an alternative host for novel anti-infectives or virulence factor discovery.It also raises issues of biosecurity due to its classification as a potential bioterrorism agent.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, 8 Medical Drive, 117597, Singapore.

ABSTRACT

Background: Burkholderia pseudomallei is the causative agent for melioidosis, a disease with significant mortality and morbidity in endemic regions. Its versatility as a pathogen is reflected in its relatively huge 7.24 Mb genome and the presence of many virulence factors including three Type Three Secretion Systems known as T3SS1, T3SS2 and T3SS3. Besides being a human pathogen, it is able to infect and cause disease in many different animals and alternative hosts such as C. elegans.

Results: Its host range is further extended to include plants as we demonstrated the ability of B. pseudomallei and the closely related species B. thailandensis to infect susceptible tomato but not rice plants. Bacteria were found to multiply intercellularly and were found in the xylem vessels of the vascular bundle. Disease is substantially attenuated upon infection with bacterial mutants deficient in T3SS1 or T3SS2 and slightly attenuated upon infection with the T3SS3 mutant. This shows the importance of both T3SS1 and T3SS2 in bacterial pathogenesis in susceptible plants.

Conclusions: The potential of B. pseudomallei as a plant pathogen raises new possibilities of exploiting plant as an alternative host for novel anti-infectives or virulence factor discovery. It also raises issues of biosecurity due to its classification as a potential bioterrorism agent.

Show MeSH
Related in: MedlinePlus