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Expression profiling of S. pombe acetyltransferase mutants identifies redundant pathways of gene regulation.

Nugent RL, Johnsson A, Fleharty B, Gogol M, Xue-Franzén Y, Seidel C, Wright AP, Forsburg SL - BMC Genomics (2010)

Bottom Line: Comparison of phenotypes and expression profiles in single, double and triple mutants indicate that these HAT enzymes have overlapping functions.Consistent with this, overlapping specificity in histone H3 acetylation is observed.However, there is no evidence for overlap with another HAT enzyme, encoded by the essential mst1+ gene.

View Article: PubMed Central - HTML - PubMed

Affiliation: Molecular and Computational Biology Section, University of Southern California, Los Angeles, California 90089-2910, USA.

ABSTRACT

Background: Histone acetyltransferase enzymes (HATs) are implicated in regulation of transcription. HATs from different families may overlap in target and substrate specificity.

Results: We isolated the elp3+ gene encoding the histone acetyltransferase subunit of the Elongator complex in fission yeast and characterized the phenotype of an Deltaelp3 mutant. We examined genetic interactions between Deltaelp3 and two other HAT mutants, Deltamst2 and Deltagcn5 and used whole genome microarray analysis to analyze their effects on gene expression.

Conclusions: Comparison of phenotypes and expression profiles in single, double and triple mutants indicate that these HAT enzymes have overlapping functions. Consistent with this, overlapping specificity in histone H3 acetylation is observed. However, there is no evidence for overlap with another HAT enzyme, encoded by the essential mst1+ gene.

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Salt response is redundantly regulated by multiple HAT families. Venn diagram of differentially down and up-regulated genes after salt stress in Δgcn5, Δmst2 and Δgcn5 Δmst2 HAT mutants as determined by the Eurogenetech array platform (Additional File 3). Strains Δgcn5 (Hu799), Δmst2 (1890) Δgcn5 Δmst2(Hu990).
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Figure 4: Salt response is redundantly regulated by multiple HAT families. Venn diagram of differentially down and up-regulated genes after salt stress in Δgcn5, Δmst2 and Δgcn5 Δmst2 HAT mutants as determined by the Eurogenetech array platform (Additional File 3). Strains Δgcn5 (Hu799), Δmst2 (1890) Δgcn5 Δmst2(Hu990).

Mentions: We found 99 genes that were significantly down-regulated and 191 genes significantly up-regulated in the double mutant, compared to Δgcn5 single mutant (Additional File 4). About 80% of these genes were not significantly regulated in either of the single mutants (Fig 4). This suggests that the two HAT enzymes overlap in the regulation of salt-responsive pathways, although there was no significant overlap in particular GO pathways.


Expression profiling of S. pombe acetyltransferase mutants identifies redundant pathways of gene regulation.

Nugent RL, Johnsson A, Fleharty B, Gogol M, Xue-Franzén Y, Seidel C, Wright AP, Forsburg SL - BMC Genomics (2010)

Salt response is redundantly regulated by multiple HAT families. Venn diagram of differentially down and up-regulated genes after salt stress in Δgcn5, Δmst2 and Δgcn5 Δmst2 HAT mutants as determined by the Eurogenetech array platform (Additional File 3). Strains Δgcn5 (Hu799), Δmst2 (1890) Δgcn5 Δmst2(Hu990).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2823694&req=5

Figure 4: Salt response is redundantly regulated by multiple HAT families. Venn diagram of differentially down and up-regulated genes after salt stress in Δgcn5, Δmst2 and Δgcn5 Δmst2 HAT mutants as determined by the Eurogenetech array platform (Additional File 3). Strains Δgcn5 (Hu799), Δmst2 (1890) Δgcn5 Δmst2(Hu990).
Mentions: We found 99 genes that were significantly down-regulated and 191 genes significantly up-regulated in the double mutant, compared to Δgcn5 single mutant (Additional File 4). About 80% of these genes were not significantly regulated in either of the single mutants (Fig 4). This suggests that the two HAT enzymes overlap in the regulation of salt-responsive pathways, although there was no significant overlap in particular GO pathways.

Bottom Line: Comparison of phenotypes and expression profiles in single, double and triple mutants indicate that these HAT enzymes have overlapping functions.Consistent with this, overlapping specificity in histone H3 acetylation is observed.However, there is no evidence for overlap with another HAT enzyme, encoded by the essential mst1+ gene.

View Article: PubMed Central - HTML - PubMed

Affiliation: Molecular and Computational Biology Section, University of Southern California, Los Angeles, California 90089-2910, USA.

ABSTRACT

Background: Histone acetyltransferase enzymes (HATs) are implicated in regulation of transcription. HATs from different families may overlap in target and substrate specificity.

Results: We isolated the elp3+ gene encoding the histone acetyltransferase subunit of the Elongator complex in fission yeast and characterized the phenotype of an Deltaelp3 mutant. We examined genetic interactions between Deltaelp3 and two other HAT mutants, Deltamst2 and Deltagcn5 and used whole genome microarray analysis to analyze their effects on gene expression.

Conclusions: Comparison of phenotypes and expression profiles in single, double and triple mutants indicate that these HAT enzymes have overlapping functions. Consistent with this, overlapping specificity in histone H3 acetylation is observed. However, there is no evidence for overlap with another HAT enzyme, encoded by the essential mst1+ gene.

Show MeSH