Limits...
Effects of crystalline glucocorticoid triamcinolone acetonide on cultered human supraspinatus tendon cells.

Tempfer H, Gehwolf R, Lehner C, Wagner A, Mtsariashvili M, Bauer HC, Resch H, Tauber M - Acta Orthop (2009)

Bottom Line: Moreover, expression and secretion of collagen I was strongly reduced, and there was a decrease in proliferation rate.Cell migration was blocked and the rate of expression of the matrix metalloproteinases MMP2, MMP8, MMP9, and MMP13 was reduced, but expression of TIMP1 (a tissue inhibitor of MMPs) was upregulated, indicating a reduction in the cellular capacity for tendon repair.These results may indicate that the use of TAA is one reason for weaker mechanical tendon properties and for the high rate of re-rupture after supraspinatus tendon repair.

View Article: PubMed Central - PubMed

Affiliation: Department of Organismic Biology, Division of Zoology and Functional Anatomy, University of Salzburg, Austria. Herbert.Tempfer@sbg.ac.at

ABSTRACT

Background: Rotator cuff tears are a common cause of shoulder pain and impairment. Subacromial glucocorticoid injections are widely used for treatment of epiphenomenons of chronic impingement syndrome with the possible side effects of tendon rupture and impaired tendon healing.

Methods: Using qRT-PCR, western blot, immunoflourescence, and measurement of 3H-thymidine uptake we investigated the effects of the crystalline glucocorticoid triamcinolone acetonide (TAA) when added to the culture medium of isolated human rotator cuff tendon cells.

Results: After 2 weeks of incubation, the cells had lost their fibroblastic appearance and parallel orientation, which is characteristic of cellular degeneration in vivo. Moreover, expression and secretion of collagen I was strongly reduced, and there was a decrease in proliferation rate. Cell migration was blocked and the rate of expression of the matrix metalloproteinases MMP2, MMP8, MMP9, and MMP13 was reduced, but expression of TIMP1 (a tissue inhibitor of MMPs) was upregulated, indicating a reduction in the cellular capacity for tendon repair. In addition, changes in cellular differentiation were observed: the number of adipocytes increased and levels of the protein Sox9-a marker of differentiating and mature chondrocytes-were elevated in triamcinolone acetonide treated cells.

Interpretation: These results may indicate that the use of TAA is one reason for weaker mechanical tendon properties and for the high rate of re-rupture after supraspinatus tendon repair.

Show MeSH

Related in: MedlinePlus

A. Downregulation of collagen type I secretion in TAA-treated supraspinatus tendon cells (STCs) (a: treated cells; b: control). The two separate bands seen in blot A are α1 chain and α2 chain of collagen type I, respectively. B. Sox9 is upregulated in treated cells (d) relative to control cells (c). C. TAA-treated cells show a decrease in 3H-thymidine uptake depending on the duration of TAA treatment. D. After TAA treatment, several cells stained positively for Sox9, whereas no positive cells were found in the control (not shown). * p < 0.001.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC2823208&req=5

Figure 0002: A. Downregulation of collagen type I secretion in TAA-treated supraspinatus tendon cells (STCs) (a: treated cells; b: control). The two separate bands seen in blot A are α1 chain and α2 chain of collagen type I, respectively. B. Sox9 is upregulated in treated cells (d) relative to control cells (c). C. TAA-treated cells show a decrease in 3H-thymidine uptake depending on the duration of TAA treatment. D. After TAA treatment, several cells stained positively for Sox9, whereas no positive cells were found in the control (not shown). * p < 0.001.

Mentions: Western blot analysis showed upregulation of Sox9 in TAA-treated cells (Figure 2). Secretion of collagen type I was reduced. Staining with Alcian blue showed positive cells in the treatment group, whereas no stained cells were found in the controls. Of 10,000 cells counted, 89 positive cells were found.


Effects of crystalline glucocorticoid triamcinolone acetonide on cultered human supraspinatus tendon cells.

Tempfer H, Gehwolf R, Lehner C, Wagner A, Mtsariashvili M, Bauer HC, Resch H, Tauber M - Acta Orthop (2009)

A. Downregulation of collagen type I secretion in TAA-treated supraspinatus tendon cells (STCs) (a: treated cells; b: control). The two separate bands seen in blot A are α1 chain and α2 chain of collagen type I, respectively. B. Sox9 is upregulated in treated cells (d) relative to control cells (c). C. TAA-treated cells show a decrease in 3H-thymidine uptake depending on the duration of TAA treatment. D. After TAA treatment, several cells stained positively for Sox9, whereas no positive cells were found in the control (not shown). * p < 0.001.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2823208&req=5

Figure 0002: A. Downregulation of collagen type I secretion in TAA-treated supraspinatus tendon cells (STCs) (a: treated cells; b: control). The two separate bands seen in blot A are α1 chain and α2 chain of collagen type I, respectively. B. Sox9 is upregulated in treated cells (d) relative to control cells (c). C. TAA-treated cells show a decrease in 3H-thymidine uptake depending on the duration of TAA treatment. D. After TAA treatment, several cells stained positively for Sox9, whereas no positive cells were found in the control (not shown). * p < 0.001.
Mentions: Western blot analysis showed upregulation of Sox9 in TAA-treated cells (Figure 2). Secretion of collagen type I was reduced. Staining with Alcian blue showed positive cells in the treatment group, whereas no stained cells were found in the controls. Of 10,000 cells counted, 89 positive cells were found.

Bottom Line: Moreover, expression and secretion of collagen I was strongly reduced, and there was a decrease in proliferation rate.Cell migration was blocked and the rate of expression of the matrix metalloproteinases MMP2, MMP8, MMP9, and MMP13 was reduced, but expression of TIMP1 (a tissue inhibitor of MMPs) was upregulated, indicating a reduction in the cellular capacity for tendon repair.These results may indicate that the use of TAA is one reason for weaker mechanical tendon properties and for the high rate of re-rupture after supraspinatus tendon repair.

View Article: PubMed Central - PubMed

Affiliation: Department of Organismic Biology, Division of Zoology and Functional Anatomy, University of Salzburg, Austria. Herbert.Tempfer@sbg.ac.at

ABSTRACT

Background: Rotator cuff tears are a common cause of shoulder pain and impairment. Subacromial glucocorticoid injections are widely used for treatment of epiphenomenons of chronic impingement syndrome with the possible side effects of tendon rupture and impaired tendon healing.

Methods: Using qRT-PCR, western blot, immunoflourescence, and measurement of 3H-thymidine uptake we investigated the effects of the crystalline glucocorticoid triamcinolone acetonide (TAA) when added to the culture medium of isolated human rotator cuff tendon cells.

Results: After 2 weeks of incubation, the cells had lost their fibroblastic appearance and parallel orientation, which is characteristic of cellular degeneration in vivo. Moreover, expression and secretion of collagen I was strongly reduced, and there was a decrease in proliferation rate. Cell migration was blocked and the rate of expression of the matrix metalloproteinases MMP2, MMP8, MMP9, and MMP13 was reduced, but expression of TIMP1 (a tissue inhibitor of MMPs) was upregulated, indicating a reduction in the cellular capacity for tendon repair. In addition, changes in cellular differentiation were observed: the number of adipocytes increased and levels of the protein Sox9-a marker of differentiating and mature chondrocytes-were elevated in triamcinolone acetonide treated cells.

Interpretation: These results may indicate that the use of TAA is one reason for weaker mechanical tendon properties and for the high rate of re-rupture after supraspinatus tendon repair.

Show MeSH
Related in: MedlinePlus