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Ultrastructural studies of gastrointestinal stromal tumors.

Park SH, Kim MK, Kim H, Song BJ, Chi JG - J. Korean Med. Sci. (2004)

Bottom Line: Normal interstitial cells of Cajal (ICC) were abundantly present around the myenteric plexuses or individually scattered through the wall of GIT.ICC was characterized by slender cytoplasmic processes, well-developed endoplasmic reticulum (ER), mitochondria, Golgi apparatus, caveolae and intermediate filaments.Compared with ICC, the GISTs and GANTs had remarkably reduced caveolae and gap junctions.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Seoul National University, College of Medicine, Seoul, Korea. shparknp@plaza.snu.ac.kr

ABSTRACT
Gastrointestinal stromal tumors (GISTs) are the most common mesenchymal tumors in the gastrointestinal tract (GIT). Although interstitial cells of Cajal has been suggested as origin of this tumor, the cytological and ultrastructural features of GISTs are heterogeneous and unclear. A total 10 cases of normal gastrointestinal tissue (control), 13 GISTs of the stomach (8), small intestine (3), mesocolon (1) and liver (1), and 2 gastrointestinal autonomic nervous tumor (GANT) of small intestine were ultrastructurally studied. Normal interstitial cells of Cajal (ICC) were abundantly present around the myenteric plexuses or individually scattered through the wall of GIT. ICC was characterized by slender cytoplasmic processes, well-developed endoplasmic reticulum (ER), mitochondria, Golgi apparatus, caveolae and intermediate filaments. The GISTs and GANTs had overlapping ultrastructures. The most common and important ultrastructural features of GISTs were rich villous cytoplasmic processes, dispersed intermediate filaments and abundant SER, and those of GANTs were neurosecretory granules and skenoid fibers. Compared with ICC, the GISTs and GANTs had remarkably reduced caveolae and gap junctions. Our study suggested that ultrastructural analysis gives much information to investigate lineage differentiation of neoplastic cells and make a differential diagnosis of these tumors from other mesenchymal tumors and between GISTs and GANTs.

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(A) Normal adult (M/45 yr) small intestine. (A-D) The immunohistochemical staining for c-Kit and CD34 shows immunoreactive interstitial cell of Cajal in myenteric plexus (A: c-Kit, ×200, B: ×400, C: ×400, D: CD34, ×400). (E-G) Ultrastructure of the interstitial cell of Cajal in normal stomach. It has slender cytoplasmic processes and the nucleus is heterochromatic and lobulated in apprearance. Two interstitial cell of Cajal hug the myenteric plexuses composed of many well-delineated peripheral nerves surrounded by Schwann cell cytoplasm and continuous external lamina (F). The cytoplasm of ICC has well-defined organelles such as rough endoplasmic reticulum, mitochondria, Golgi apparatus and caveolae. Gap junctions (arrows) are present but no external lamina is noted (G). (uranyl acetate and lead citrate stain E-F: bar: 2 µm, G: bar: 1 µm, E: ×2,500, F: ×3,000, G: ×8,000).
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Figure 4: (A) Normal adult (M/45 yr) small intestine. (A-D) The immunohistochemical staining for c-Kit and CD34 shows immunoreactive interstitial cell of Cajal in myenteric plexus (A: c-Kit, ×200, B: ×400, C: ×400, D: CD34, ×400). (E-G) Ultrastructure of the interstitial cell of Cajal in normal stomach. It has slender cytoplasmic processes and the nucleus is heterochromatic and lobulated in apprearance. Two interstitial cell of Cajal hug the myenteric plexuses composed of many well-delineated peripheral nerves surrounded by Schwann cell cytoplasm and continuous external lamina (F). The cytoplasm of ICC has well-defined organelles such as rough endoplasmic reticulum, mitochondria, Golgi apparatus and caveolae. Gap junctions (arrows) are present but no external lamina is noted (G). (uranyl acetate and lead citrate stain E-F: bar: 2 µm, G: bar: 1 µm, E: ×2,500, F: ×3,000, G: ×8,000).

Mentions: In normal adults stomach and intestine, well-developed c-Kit immunoreactive ICC were present around the myenteric plexus and muscle layers (Fig. 4). They were also immunoreactive for CD34 (Fig. 4).


Ultrastructural studies of gastrointestinal stromal tumors.

Park SH, Kim MK, Kim H, Song BJ, Chi JG - J. Korean Med. Sci. (2004)

(A) Normal adult (M/45 yr) small intestine. (A-D) The immunohistochemical staining for c-Kit and CD34 shows immunoreactive interstitial cell of Cajal in myenteric plexus (A: c-Kit, ×200, B: ×400, C: ×400, D: CD34, ×400). (E-G) Ultrastructure of the interstitial cell of Cajal in normal stomach. It has slender cytoplasmic processes and the nucleus is heterochromatic and lobulated in apprearance. Two interstitial cell of Cajal hug the myenteric plexuses composed of many well-delineated peripheral nerves surrounded by Schwann cell cytoplasm and continuous external lamina (F). The cytoplasm of ICC has well-defined organelles such as rough endoplasmic reticulum, mitochondria, Golgi apparatus and caveolae. Gap junctions (arrows) are present but no external lamina is noted (G). (uranyl acetate and lead citrate stain E-F: bar: 2 µm, G: bar: 1 µm, E: ×2,500, F: ×3,000, G: ×8,000).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2822305&req=5

Figure 4: (A) Normal adult (M/45 yr) small intestine. (A-D) The immunohistochemical staining for c-Kit and CD34 shows immunoreactive interstitial cell of Cajal in myenteric plexus (A: c-Kit, ×200, B: ×400, C: ×400, D: CD34, ×400). (E-G) Ultrastructure of the interstitial cell of Cajal in normal stomach. It has slender cytoplasmic processes and the nucleus is heterochromatic and lobulated in apprearance. Two interstitial cell of Cajal hug the myenteric plexuses composed of many well-delineated peripheral nerves surrounded by Schwann cell cytoplasm and continuous external lamina (F). The cytoplasm of ICC has well-defined organelles such as rough endoplasmic reticulum, mitochondria, Golgi apparatus and caveolae. Gap junctions (arrows) are present but no external lamina is noted (G). (uranyl acetate and lead citrate stain E-F: bar: 2 µm, G: bar: 1 µm, E: ×2,500, F: ×3,000, G: ×8,000).
Mentions: In normal adults stomach and intestine, well-developed c-Kit immunoreactive ICC were present around the myenteric plexus and muscle layers (Fig. 4). They were also immunoreactive for CD34 (Fig. 4).

Bottom Line: Normal interstitial cells of Cajal (ICC) were abundantly present around the myenteric plexuses or individually scattered through the wall of GIT.ICC was characterized by slender cytoplasmic processes, well-developed endoplasmic reticulum (ER), mitochondria, Golgi apparatus, caveolae and intermediate filaments.Compared with ICC, the GISTs and GANTs had remarkably reduced caveolae and gap junctions.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Seoul National University, College of Medicine, Seoul, Korea. shparknp@plaza.snu.ac.kr

ABSTRACT
Gastrointestinal stromal tumors (GISTs) are the most common mesenchymal tumors in the gastrointestinal tract (GIT). Although interstitial cells of Cajal has been suggested as origin of this tumor, the cytological and ultrastructural features of GISTs are heterogeneous and unclear. A total 10 cases of normal gastrointestinal tissue (control), 13 GISTs of the stomach (8), small intestine (3), mesocolon (1) and liver (1), and 2 gastrointestinal autonomic nervous tumor (GANT) of small intestine were ultrastructurally studied. Normal interstitial cells of Cajal (ICC) were abundantly present around the myenteric plexuses or individually scattered through the wall of GIT. ICC was characterized by slender cytoplasmic processes, well-developed endoplasmic reticulum (ER), mitochondria, Golgi apparatus, caveolae and intermediate filaments. The GISTs and GANTs had overlapping ultrastructures. The most common and important ultrastructural features of GISTs were rich villous cytoplasmic processes, dispersed intermediate filaments and abundant SER, and those of GANTs were neurosecretory granules and skenoid fibers. Compared with ICC, the GISTs and GANTs had remarkably reduced caveolae and gap junctions. Our study suggested that ultrastructural analysis gives much information to investigate lineage differentiation of neoplastic cells and make a differential diagnosis of these tumors from other mesenchymal tumors and between GISTs and GANTs.

Show MeSH
Related in: MedlinePlus