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Airway inflammation and allergen specific IgE production may persist longer than airway hyperresponsiveness in mice.

Chang YS, Kim YK, Kim TB, Kang HR, Kim SS, Bahn JW, Min KU, Kim YY, Cho SH - J. Korean Med. Sci. (2004)

Bottom Line: Each group of mice was rechallenged at 5, 7, 9, or 12 weeks after the initial challenge.Airway eosinophilia, airway inflammation and serum ovalbumin-specific IgE production persisted upon secondary allergen challenges at least 12 weeks after the initial challenge.However, airway hyperresponsiveness persisted only until mice were rechallenged 7 weeks after the initial challenge.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine, Seoul National University College of Medicine, 28 Yongon-dong, Chongno-gu, Seoul 110-744, Korea.

ABSTRACT
During the preclinical study of new therapeutic modality, we evaluate whether the treatment can reverse the established asthma phenotypes in animal model. However, few have reported on the long term persistence of asthma phenotypes upon re-challenge with allergen (secondary challenge) in animal model. We evaluated the persistence of asthma phenotypes by secondary challenge at different times in previously challenged murine asthma model. BALB/c mice sensitized by intraperitoneal injections of 20 micro g of ovalbumin and 1 mg of alum on days 1 and 14 were challenged initially by the inhalation of 1% ovalbumin for 30 min on days 21, 22, and 23. Each group of mice was rechallenged at 5, 7, 9, or 12 weeks after the initial challenge. Airway hyperresponsiveness, BAL fluid, airway histology and serum ovalbumin-specific IgE level were evaluated. Airway eosinophilia, airway inflammation and serum ovalbumin-specific IgE production persisted upon secondary allergen challenges at least 12 weeks after the initial challenge. However, airway hyperresponsiveness persisted only until mice were rechallenged 7 weeks after the initial challenge. Airway inflammation and allergen specific IgE production may persist longer than airway hyperresponsiveness in a mouse asthma model of secondary allergen challenge.

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Related in: MedlinePlus

(A) The proportion of eosinophils in bronchoalveolar lavage fluid. Eosinophils are increased even when mice were rechallenged 12 weeks after the initial challenge. (*p<0.05, compared to the PBS treated mice, BALF: bronchoalveolar lavage fluid). (B) The degrees of airway inflammation observed in the initially and rechallenged animals at 5, 7, 9, or 12 weeks after the initial challenge are similar each other and higher than that of the PBS treated mice. Total lung inflammation was defined as the average value of the peribronchial and perivascular inflammation scores. (*p<0.05, compared to initially challenged mice).
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Figure 3: (A) The proportion of eosinophils in bronchoalveolar lavage fluid. Eosinophils are increased even when mice were rechallenged 12 weeks after the initial challenge. (*p<0.05, compared to the PBS treated mice, BALF: bronchoalveolar lavage fluid). (B) The degrees of airway inflammation observed in the initially and rechallenged animals at 5, 7, 9, or 12 weeks after the initial challenge are similar each other and higher than that of the PBS treated mice. Total lung inflammation was defined as the average value of the peribronchial and perivascular inflammation scores. (*p<0.05, compared to initially challenged mice).

Mentions: The percentages of BALF eosinophils in the secondarily challenged mice were higher even in mice that were rechallenged 12 weeks after initial challenge. The proportions of BALF eosinophils observed in the rechallenged animals at 5, 7, 9, or 12 weeks after the initial challenge were similar to that of initially challenged group (37.8±9.7, 34.7±4.6, 29.4±9.1, 23.3±6.0 vs. 39.6±9.3 %, p>0.05). PBS treated mice showed no eosinophil in BAL fluid at initial challenge and even at the end of the study (0.0±0.0%). (Fig. 3A)


Airway inflammation and allergen specific IgE production may persist longer than airway hyperresponsiveness in mice.

Chang YS, Kim YK, Kim TB, Kang HR, Kim SS, Bahn JW, Min KU, Kim YY, Cho SH - J. Korean Med. Sci. (2004)

(A) The proportion of eosinophils in bronchoalveolar lavage fluid. Eosinophils are increased even when mice were rechallenged 12 weeks after the initial challenge. (*p<0.05, compared to the PBS treated mice, BALF: bronchoalveolar lavage fluid). (B) The degrees of airway inflammation observed in the initially and rechallenged animals at 5, 7, 9, or 12 weeks after the initial challenge are similar each other and higher than that of the PBS treated mice. Total lung inflammation was defined as the average value of the peribronchial and perivascular inflammation scores. (*p<0.05, compared to initially challenged mice).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2822266&req=5

Figure 3: (A) The proportion of eosinophils in bronchoalveolar lavage fluid. Eosinophils are increased even when mice were rechallenged 12 weeks after the initial challenge. (*p<0.05, compared to the PBS treated mice, BALF: bronchoalveolar lavage fluid). (B) The degrees of airway inflammation observed in the initially and rechallenged animals at 5, 7, 9, or 12 weeks after the initial challenge are similar each other and higher than that of the PBS treated mice. Total lung inflammation was defined as the average value of the peribronchial and perivascular inflammation scores. (*p<0.05, compared to initially challenged mice).
Mentions: The percentages of BALF eosinophils in the secondarily challenged mice were higher even in mice that were rechallenged 12 weeks after initial challenge. The proportions of BALF eosinophils observed in the rechallenged animals at 5, 7, 9, or 12 weeks after the initial challenge were similar to that of initially challenged group (37.8±9.7, 34.7±4.6, 29.4±9.1, 23.3±6.0 vs. 39.6±9.3 %, p>0.05). PBS treated mice showed no eosinophil in BAL fluid at initial challenge and even at the end of the study (0.0±0.0%). (Fig. 3A)

Bottom Line: Each group of mice was rechallenged at 5, 7, 9, or 12 weeks after the initial challenge.Airway eosinophilia, airway inflammation and serum ovalbumin-specific IgE production persisted upon secondary allergen challenges at least 12 weeks after the initial challenge.However, airway hyperresponsiveness persisted only until mice were rechallenged 7 weeks after the initial challenge.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine, Seoul National University College of Medicine, 28 Yongon-dong, Chongno-gu, Seoul 110-744, Korea.

ABSTRACT
During the preclinical study of new therapeutic modality, we evaluate whether the treatment can reverse the established asthma phenotypes in animal model. However, few have reported on the long term persistence of asthma phenotypes upon re-challenge with allergen (secondary challenge) in animal model. We evaluated the persistence of asthma phenotypes by secondary challenge at different times in previously challenged murine asthma model. BALB/c mice sensitized by intraperitoneal injections of 20 micro g of ovalbumin and 1 mg of alum on days 1 and 14 were challenged initially by the inhalation of 1% ovalbumin for 30 min on days 21, 22, and 23. Each group of mice was rechallenged at 5, 7, 9, or 12 weeks after the initial challenge. Airway hyperresponsiveness, BAL fluid, airway histology and serum ovalbumin-specific IgE level were evaluated. Airway eosinophilia, airway inflammation and serum ovalbumin-specific IgE production persisted upon secondary allergen challenges at least 12 weeks after the initial challenge. However, airway hyperresponsiveness persisted only until mice were rechallenged 7 weeks after the initial challenge. Airway inflammation and allergen specific IgE production may persist longer than airway hyperresponsiveness in a mouse asthma model of secondary allergen challenge.

Show MeSH
Related in: MedlinePlus