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Effects of propofol on endotoxin-induced acute lung injury in rabbit.

Kwak SH, Choi JI, Park JT - J. Korean Med. Sci. (2004)

Bottom Line: Endotoxin decreased PaO2, and peripheral blood leukocyte and platelet count.And it increased W/D ratio of lung, lung injury score and leukocyte count, percentage of PMN cells, concentration of albumin, thromboxane B2 and IL-8 in BALF.Propofol attenuated all these changes except the leukocyte count in peripheral blood.

View Article: PubMed Central - PubMed

Affiliation: Department of Anesthesiology and Pain Medicine, Chonnam National University Medical School, 8 Hakdong, Dong-gu, Gwangju 501-757, Korea. s shkwak@chonnam.ac.kr

ABSTRACT
This study was undertaken to clarify the effects of propofol on endotoxin-induced acute lung injury. Rabbits were randomly assigned to one of four groups. Each group received intravenous infusion of saline only, saline and Escherichia coli endotoxin, propofol (1 mg/kg bolus, then 5 mg/kg/hr) and endotoxin, or propofol (4 mg/kg bolus, then 20 mg/kg/hr) and endotoxin respectively. Infusion of saline or propofol was started 0.5 hr before the infusion of saline or endotoxin, and continued for 6 hr thereafter. The lungs of rabbits were ventilated with 40% oxygen. Mean blood pressure, heart rate, arterial oxygen tension (PaO2), and peripheral blood leukocyte and platelet count were recorded. The wet/dry (W/D) weight ratio of lung and lung injury score were measured, and analysis of bronchoalveolar lavage fluid (BALF) was done. Endotoxin decreased PaO2, and peripheral blood leukocyte and platelet count. And it increased W/D ratio of lung, lung injury score and leukocyte count, percentage of PMN cells, concentration of albumin, thromboxane B2 and IL-8 in BALF. Propofol attenuated all these changes except the leukocyte count in peripheral blood. In conclusion, propofol attenuated endotoxin-induced acute lung injury in rabbits mainly by inhibiting neutrophil and IL-8 responses, which may play a central role in sepsis-related lung injury.

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Effects of propofol on lung tissue damage 6 hr after the start of saline or endotoxin infusion. Representive photomicrographs show rabbit's lung with median values of lung injury score in (A) group S-S, (B) group S-E, and (C) group P-E (H&E, ×400). Lung injury score (D) was recorded from 0 (no damage) to 4+ (maximum damage) according to the criteria described in Materials and Methods. Each bar represents median value of seven rabbits in each group. *p<0.05 versus group S-S. †p<0.05 for group P5-E or P20-E versus group S-E.
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Figure 2: Effects of propofol on lung tissue damage 6 hr after the start of saline or endotoxin infusion. Representive photomicrographs show rabbit's lung with median values of lung injury score in (A) group S-S, (B) group S-E, and (C) group P-E (H&E, ×400). Lung injury score (D) was recorded from 0 (no damage) to 4+ (maximum damage) according to the criteria described in Materials and Methods. Each bar represents median value of seven rabbits in each group. *p<0.05 versus group S-S. †p<0.05 for group P5-E or P20-E versus group S-E.

Mentions: Light microscopic findings in S-E group include edema, hemorrhage, thickening of the alveolar wall, and infiltration of inflammatory cells into alveolar spaces. In contrast, these changes were less prominent in the rabbits receiving propofol. Assessment of the lung injury scores demonstrated that propofol successfully reduced the histopathologic severity of the lung injury (Fig. 2).


Effects of propofol on endotoxin-induced acute lung injury in rabbit.

Kwak SH, Choi JI, Park JT - J. Korean Med. Sci. (2004)

Effects of propofol on lung tissue damage 6 hr after the start of saline or endotoxin infusion. Representive photomicrographs show rabbit's lung with median values of lung injury score in (A) group S-S, (B) group S-E, and (C) group P-E (H&E, ×400). Lung injury score (D) was recorded from 0 (no damage) to 4+ (maximum damage) according to the criteria described in Materials and Methods. Each bar represents median value of seven rabbits in each group. *p<0.05 versus group S-S. †p<0.05 for group P5-E or P20-E versus group S-E.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2822264&req=5

Figure 2: Effects of propofol on lung tissue damage 6 hr after the start of saline or endotoxin infusion. Representive photomicrographs show rabbit's lung with median values of lung injury score in (A) group S-S, (B) group S-E, and (C) group P-E (H&E, ×400). Lung injury score (D) was recorded from 0 (no damage) to 4+ (maximum damage) according to the criteria described in Materials and Methods. Each bar represents median value of seven rabbits in each group. *p<0.05 versus group S-S. †p<0.05 for group P5-E or P20-E versus group S-E.
Mentions: Light microscopic findings in S-E group include edema, hemorrhage, thickening of the alveolar wall, and infiltration of inflammatory cells into alveolar spaces. In contrast, these changes were less prominent in the rabbits receiving propofol. Assessment of the lung injury scores demonstrated that propofol successfully reduced the histopathologic severity of the lung injury (Fig. 2).

Bottom Line: Endotoxin decreased PaO2, and peripheral blood leukocyte and platelet count.And it increased W/D ratio of lung, lung injury score and leukocyte count, percentage of PMN cells, concentration of albumin, thromboxane B2 and IL-8 in BALF.Propofol attenuated all these changes except the leukocyte count in peripheral blood.

View Article: PubMed Central - PubMed

Affiliation: Department of Anesthesiology and Pain Medicine, Chonnam National University Medical School, 8 Hakdong, Dong-gu, Gwangju 501-757, Korea. s shkwak@chonnam.ac.kr

ABSTRACT
This study was undertaken to clarify the effects of propofol on endotoxin-induced acute lung injury. Rabbits were randomly assigned to one of four groups. Each group received intravenous infusion of saline only, saline and Escherichia coli endotoxin, propofol (1 mg/kg bolus, then 5 mg/kg/hr) and endotoxin, or propofol (4 mg/kg bolus, then 20 mg/kg/hr) and endotoxin respectively. Infusion of saline or propofol was started 0.5 hr before the infusion of saline or endotoxin, and continued for 6 hr thereafter. The lungs of rabbits were ventilated with 40% oxygen. Mean blood pressure, heart rate, arterial oxygen tension (PaO2), and peripheral blood leukocyte and platelet count were recorded. The wet/dry (W/D) weight ratio of lung and lung injury score were measured, and analysis of bronchoalveolar lavage fluid (BALF) was done. Endotoxin decreased PaO2, and peripheral blood leukocyte and platelet count. And it increased W/D ratio of lung, lung injury score and leukocyte count, percentage of PMN cells, concentration of albumin, thromboxane B2 and IL-8 in BALF. Propofol attenuated all these changes except the leukocyte count in peripheral blood. In conclusion, propofol attenuated endotoxin-induced acute lung injury in rabbits mainly by inhibiting neutrophil and IL-8 responses, which may play a central role in sepsis-related lung injury.

Show MeSH
Related in: MedlinePlus