Limits...
Effects of propofol on endotoxin-induced acute lung injury in rabbit.

Kwak SH, Choi JI, Park JT - J. Korean Med. Sci. (2004)

Bottom Line: Endotoxin decreased PaO2, and peripheral blood leukocyte and platelet count.And it increased W/D ratio of lung, lung injury score and leukocyte count, percentage of PMN cells, concentration of albumin, thromboxane B2 and IL-8 in BALF.Propofol attenuated all these changes except the leukocyte count in peripheral blood.

View Article: PubMed Central - PubMed

Affiliation: Department of Anesthesiology and Pain Medicine, Chonnam National University Medical School, 8 Hakdong, Dong-gu, Gwangju 501-757, Korea. s shkwak@chonnam.ac.kr

ABSTRACT
This study was undertaken to clarify the effects of propofol on endotoxin-induced acute lung injury. Rabbits were randomly assigned to one of four groups. Each group received intravenous infusion of saline only, saline and Escherichia coli endotoxin, propofol (1 mg/kg bolus, then 5 mg/kg/hr) and endotoxin, or propofol (4 mg/kg bolus, then 20 mg/kg/hr) and endotoxin respectively. Infusion of saline or propofol was started 0.5 hr before the infusion of saline or endotoxin, and continued for 6 hr thereafter. The lungs of rabbits were ventilated with 40% oxygen. Mean blood pressure, heart rate, arterial oxygen tension (PaO2), and peripheral blood leukocyte and platelet count were recorded. The wet/dry (W/D) weight ratio of lung and lung injury score were measured, and analysis of bronchoalveolar lavage fluid (BALF) was done. Endotoxin decreased PaO2, and peripheral blood leukocyte and platelet count. And it increased W/D ratio of lung, lung injury score and leukocyte count, percentage of PMN cells, concentration of albumin, thromboxane B2 and IL-8 in BALF. Propofol attenuated all these changes except the leukocyte count in peripheral blood. In conclusion, propofol attenuated endotoxin-induced acute lung injury in rabbits mainly by inhibiting neutrophil and IL-8 responses, which may play a central role in sepsis-related lung injury.

Show MeSH

Related in: MedlinePlus

Comparison of changes in arterial oxygen tension (PaO2). S-S, saline control group; S-E, endotoxin control group; P5-E, low dose propofol treated group; P-20E, high dose propofol treated group. Horizontal axis indicates the time period after the start of saline or endotoxin infusion. Each point represents mean±SEM of seven rabbits. *p<0.05 versus time matched values in S-S group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC2822264&req=5

Figure 1: Comparison of changes in arterial oxygen tension (PaO2). S-S, saline control group; S-E, endotoxin control group; P5-E, low dose propofol treated group; P-20E, high dose propofol treated group. Horizontal axis indicates the time period after the start of saline or endotoxin infusion. Each point represents mean±SEM of seven rabbits. *p<0.05 versus time matched values in S-S group.

Mentions: The PaO2, pH, base excess, peripheral blood leukocyte and platelet counts were decreased gradually in S-E group, compared with S-S group. Propofol attenuated the decrease in the PaO2 (Fig. 1). But, the changes in the pH, base excess, and platelet count were attenuated only in P20-E group but not in P5-E group (Table 1). The PaCO2, mean arterial pressure and heart rate were not significantly changed at any study point in the four groups (data not shown).


Effects of propofol on endotoxin-induced acute lung injury in rabbit.

Kwak SH, Choi JI, Park JT - J. Korean Med. Sci. (2004)

Comparison of changes in arterial oxygen tension (PaO2). S-S, saline control group; S-E, endotoxin control group; P5-E, low dose propofol treated group; P-20E, high dose propofol treated group. Horizontal axis indicates the time period after the start of saline or endotoxin infusion. Each point represents mean±SEM of seven rabbits. *p<0.05 versus time matched values in S-S group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2822264&req=5

Figure 1: Comparison of changes in arterial oxygen tension (PaO2). S-S, saline control group; S-E, endotoxin control group; P5-E, low dose propofol treated group; P-20E, high dose propofol treated group. Horizontal axis indicates the time period after the start of saline or endotoxin infusion. Each point represents mean±SEM of seven rabbits. *p<0.05 versus time matched values in S-S group.
Mentions: The PaO2, pH, base excess, peripheral blood leukocyte and platelet counts were decreased gradually in S-E group, compared with S-S group. Propofol attenuated the decrease in the PaO2 (Fig. 1). But, the changes in the pH, base excess, and platelet count were attenuated only in P20-E group but not in P5-E group (Table 1). The PaCO2, mean arterial pressure and heart rate were not significantly changed at any study point in the four groups (data not shown).

Bottom Line: Endotoxin decreased PaO2, and peripheral blood leukocyte and platelet count.And it increased W/D ratio of lung, lung injury score and leukocyte count, percentage of PMN cells, concentration of albumin, thromboxane B2 and IL-8 in BALF.Propofol attenuated all these changes except the leukocyte count in peripheral blood.

View Article: PubMed Central - PubMed

Affiliation: Department of Anesthesiology and Pain Medicine, Chonnam National University Medical School, 8 Hakdong, Dong-gu, Gwangju 501-757, Korea. s shkwak@chonnam.ac.kr

ABSTRACT
This study was undertaken to clarify the effects of propofol on endotoxin-induced acute lung injury. Rabbits were randomly assigned to one of four groups. Each group received intravenous infusion of saline only, saline and Escherichia coli endotoxin, propofol (1 mg/kg bolus, then 5 mg/kg/hr) and endotoxin, or propofol (4 mg/kg bolus, then 20 mg/kg/hr) and endotoxin respectively. Infusion of saline or propofol was started 0.5 hr before the infusion of saline or endotoxin, and continued for 6 hr thereafter. The lungs of rabbits were ventilated with 40% oxygen. Mean blood pressure, heart rate, arterial oxygen tension (PaO2), and peripheral blood leukocyte and platelet count were recorded. The wet/dry (W/D) weight ratio of lung and lung injury score were measured, and analysis of bronchoalveolar lavage fluid (BALF) was done. Endotoxin decreased PaO2, and peripheral blood leukocyte and platelet count. And it increased W/D ratio of lung, lung injury score and leukocyte count, percentage of PMN cells, concentration of albumin, thromboxane B2 and IL-8 in BALF. Propofol attenuated all these changes except the leukocyte count in peripheral blood. In conclusion, propofol attenuated endotoxin-induced acute lung injury in rabbits mainly by inhibiting neutrophil and IL-8 responses, which may play a central role in sepsis-related lung injury.

Show MeSH
Related in: MedlinePlus