Limits...
Continuous brain-derived neurotrophic factor (BDNF) infusion after methylprednisolone treatment in severe spinal cord injury.

Kim DH, Jahng TA - J. Korean Med. Sci. (2004)

Bottom Line: Immunohistochemical methods were used to detect ED-1, Growth associated protein-43 (GAP-43), neurofilament (NF), and choline acethyl transferase (ChAT) levels.BDNF did not alter the effect of MP on hematogenous inflammatory cellular infiltration.Adjunctive BDNF infusion resulted in better locomotor test scores using the Basso-Beattie-Bresnahan (BBB) test.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurosurgery, Stanford University School of Medicine, Stanford, CA, USA. spine@snuh.org

ABSTRACT
Although methylprednisolone (MP) is the standard of care in acute spinal cord injury (SCI), its functional outcome varies in clinical situation. Recent report demonstrated that MP depresses the expression of growth-promoting neurotrophic factors after acute SCI. The present study was designed to investigate whether continuous infusion of brain-derived neurotrophic factor (BDNF) after MP treatment promotes functional recovery in severe SCI. Contusion injury was produced at the T10 vertebral level of the spinal cord in adult rats. The rats received MP intravenously immediately after the injury and BDNF was infused intrathecally using an osmotic mini-pump for six weeks. Immunohistochemical methods were used to detect ED-1, Growth associated protein-43 (GAP-43), neurofilament (NF), and choline acethyl transferase (ChAT) levels. BDNF did not alter the effect of MP on hematogenous inflammatory cellular infiltration. MP treatment with BDNF infusion resulted in greater axonal survival and regeneration compared to MP treatment alone, as indicated by increases in NF and GAP-43 gene expression. Adjunctive BDNF infusion resulted in better locomotor test scores using the Basso-Beattie-Bresnahan (BBB) test. This study demonstrated that continuous infusion of BDNF after initial MP treatment improved functional recovery after severe spinal cord injury without dampening the acute effect of MP.

Show MeSH

Related in: MedlinePlus

Immunohistochemical staining for ChAT at the contusion injury site (×40). More ChAT-positive axons appear in MP+BDNF rats (C) ten weeks after SCI than in the other two groups (A, B). This difference, represented as means±S.D. in (D), is significant (p<0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC2822246&req=5

Figure 4: Immunohistochemical staining for ChAT at the contusion injury site (×40). More ChAT-positive axons appear in MP+BDNF rats (C) ten weeks after SCI than in the other two groups (A, B). This difference, represented as means±S.D. in (D), is significant (p<0.05).

Mentions: To identify the phenotype of regenerating fibers, ChAT immunoreactivity was assessed. As shown in Fig. 4, ChAT staining showed that motor axons predominated in axonal regeneration, and the MP+BDNF-infused group (Fig. 4C) regenerated many more motor axons than did the MP-only and control groups (Fig. 4A, B, D; p<0.05).


Continuous brain-derived neurotrophic factor (BDNF) infusion after methylprednisolone treatment in severe spinal cord injury.

Kim DH, Jahng TA - J. Korean Med. Sci. (2004)

Immunohistochemical staining for ChAT at the contusion injury site (×40). More ChAT-positive axons appear in MP+BDNF rats (C) ten weeks after SCI than in the other two groups (A, B). This difference, represented as means±S.D. in (D), is significant (p<0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2822246&req=5

Figure 4: Immunohistochemical staining for ChAT at the contusion injury site (×40). More ChAT-positive axons appear in MP+BDNF rats (C) ten weeks after SCI than in the other two groups (A, B). This difference, represented as means±S.D. in (D), is significant (p<0.05).
Mentions: To identify the phenotype of regenerating fibers, ChAT immunoreactivity was assessed. As shown in Fig. 4, ChAT staining showed that motor axons predominated in axonal regeneration, and the MP+BDNF-infused group (Fig. 4C) regenerated many more motor axons than did the MP-only and control groups (Fig. 4A, B, D; p<0.05).

Bottom Line: Immunohistochemical methods were used to detect ED-1, Growth associated protein-43 (GAP-43), neurofilament (NF), and choline acethyl transferase (ChAT) levels.BDNF did not alter the effect of MP on hematogenous inflammatory cellular infiltration.Adjunctive BDNF infusion resulted in better locomotor test scores using the Basso-Beattie-Bresnahan (BBB) test.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurosurgery, Stanford University School of Medicine, Stanford, CA, USA. spine@snuh.org

ABSTRACT
Although methylprednisolone (MP) is the standard of care in acute spinal cord injury (SCI), its functional outcome varies in clinical situation. Recent report demonstrated that MP depresses the expression of growth-promoting neurotrophic factors after acute SCI. The present study was designed to investigate whether continuous infusion of brain-derived neurotrophic factor (BDNF) after MP treatment promotes functional recovery in severe SCI. Contusion injury was produced at the T10 vertebral level of the spinal cord in adult rats. The rats received MP intravenously immediately after the injury and BDNF was infused intrathecally using an osmotic mini-pump for six weeks. Immunohistochemical methods were used to detect ED-1, Growth associated protein-43 (GAP-43), neurofilament (NF), and choline acethyl transferase (ChAT) levels. BDNF did not alter the effect of MP on hematogenous inflammatory cellular infiltration. MP treatment with BDNF infusion resulted in greater axonal survival and regeneration compared to MP treatment alone, as indicated by increases in NF and GAP-43 gene expression. Adjunctive BDNF infusion resulted in better locomotor test scores using the Basso-Beattie-Bresnahan (BBB) test. This study demonstrated that continuous infusion of BDNF after initial MP treatment improved functional recovery after severe spinal cord injury without dampening the acute effect of MP.

Show MeSH
Related in: MedlinePlus