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Budding of Taenia crassiceps cysticerci in vitro is promoted by crowding in addition to hormonal, stress, and energy-related signals.

Ostoa-Saloma P, Ostoa-Jacobo P, Esquivel-Velázquez M, Bazúa S, Larralde C - J. Biomed. Biotechnol. (2010)

Bottom Line: Results showed that there is considerable intrinsic initial variation among inoculated cysts in their production of buds and that increasing parasite density (crowding) stimulates the overall production of buds and recruit into budding most of the cysts.Identical cultures were then subjected to various treatments such as heating and exposure to peroxide to induce stress, or to 17beta-estradiol, insulin, glucose, or insulin+glucose to supplement putatively limiting hormonal and energy resources.All treatments increased budding but the parasites' strong budding response to crowding alone overshadows the other treatments.

View Article: PubMed Central - PubMed

Affiliation: Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, A.P. 70228, C.P. 04510, México D.F., Mexico. postoa@unam.mx

ABSTRACT
Taenia crassiceps cysticerci (cysts) reproduce by budding. The cysts' production of buds was measured in vitro to explore parasite and environmental-related factors involved in the extreme individual variation in parasite loads of inbred mice. Cysts were placed in in vitro culture for 10 days at initial parasite densities of 1, 5, 10 cysts/well in 1 ml of RPMI Medium 1640 without serum. Results showed that there is considerable intrinsic initial variation among inoculated cysts in their production of buds and that increasing parasite density (crowding) stimulates the overall production of buds and recruit into budding most of the cysts. Identical cultures were then subjected to various treatments such as heating and exposure to peroxide to induce stress, or to 17beta-estradiol, insulin, glucose, or insulin+glucose to supplement putatively limiting hormonal and energy resources. All treatments increased budding but the parasites' strong budding response to crowding alone overshadows the other treatments.

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Related in: MedlinePlus

Budding process at the different conditions from day 0 to day 10. The cultured cysticerci from Donor number 2 were submitted to standard stressing (heat and peroxide) or favorable conditions (addition of 17ß-estradiol, insulin, glucose, insulin+glucose) and cultured in vitro as done in Experiment number 1. The results presented are from one experiment with its duplicate for each density. The graphic expresses the average value plus standard deviations. In d = 1 and d = 5, the budding in the presence of glucose and peroxide is significative. In d = 10 the budding in the presence of estradiol and insuline+glucose is significative.
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fig2: Budding process at the different conditions from day 0 to day 10. The cultured cysticerci from Donor number 2 were submitted to standard stressing (heat and peroxide) or favorable conditions (addition of 17ß-estradiol, insulin, glucose, insulin+glucose) and cultured in vitro as done in Experiment number 1. The results presented are from one experiment with its duplicate for each density. The graphic expresses the average value plus standard deviations. In d = 1 and d = 5, the budding in the presence of glucose and peroxide is significative. In d = 10 the budding in the presence of estradiol and insuline+glucose is significative.

Mentions: Experiment number 2 was designed to address those questions, bearing in mind that increasing density may decrease resource availability and lead the parasites to enter into stress. Accordingly, the cultured cysticerci from Donor number 2 were submitted to standard stress (heat and peroxide) or favorable conditions (addition of 17ß-estradiol, insulin, glucose, insulin+glucose) and cultured in vitro as done in Experiment number 1. Figure 2 shows the budding process at the different conditions from day 0 to day 10. Table I most clearly shows that the total number of buds/cyst produced in vitro are increased about twofold with respect to unsupplemented control values at densities 1 and 5 but not at density 10, which is in fact reduced by the supplementations. The great difference between the control values of ∑buds/cyst in Experiments number 1 and number 2 (0.17 and 3.0, resp.) speaks of there being such differences between the harvested cysticerci from the two donor mice in the cysts' “readiness to bud,” possibly depending on the state and terms of each host-parasite relationship established with the donor mice.


Budding of Taenia crassiceps cysticerci in vitro is promoted by crowding in addition to hormonal, stress, and energy-related signals.

Ostoa-Saloma P, Ostoa-Jacobo P, Esquivel-Velázquez M, Bazúa S, Larralde C - J. Biomed. Biotechnol. (2010)

Budding process at the different conditions from day 0 to day 10. The cultured cysticerci from Donor number 2 were submitted to standard stressing (heat and peroxide) or favorable conditions (addition of 17ß-estradiol, insulin, glucose, insulin+glucose) and cultured in vitro as done in Experiment number 1. The results presented are from one experiment with its duplicate for each density. The graphic expresses the average value plus standard deviations. In d = 1 and d = 5, the budding in the presence of glucose and peroxide is significative. In d = 10 the budding in the presence of estradiol and insuline+glucose is significative.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2822235&req=5

fig2: Budding process at the different conditions from day 0 to day 10. The cultured cysticerci from Donor number 2 were submitted to standard stressing (heat and peroxide) or favorable conditions (addition of 17ß-estradiol, insulin, glucose, insulin+glucose) and cultured in vitro as done in Experiment number 1. The results presented are from one experiment with its duplicate for each density. The graphic expresses the average value plus standard deviations. In d = 1 and d = 5, the budding in the presence of glucose and peroxide is significative. In d = 10 the budding in the presence of estradiol and insuline+glucose is significative.
Mentions: Experiment number 2 was designed to address those questions, bearing in mind that increasing density may decrease resource availability and lead the parasites to enter into stress. Accordingly, the cultured cysticerci from Donor number 2 were submitted to standard stress (heat and peroxide) or favorable conditions (addition of 17ß-estradiol, insulin, glucose, insulin+glucose) and cultured in vitro as done in Experiment number 1. Figure 2 shows the budding process at the different conditions from day 0 to day 10. Table I most clearly shows that the total number of buds/cyst produced in vitro are increased about twofold with respect to unsupplemented control values at densities 1 and 5 but not at density 10, which is in fact reduced by the supplementations. The great difference between the control values of ∑buds/cyst in Experiments number 1 and number 2 (0.17 and 3.0, resp.) speaks of there being such differences between the harvested cysticerci from the two donor mice in the cysts' “readiness to bud,” possibly depending on the state and terms of each host-parasite relationship established with the donor mice.

Bottom Line: Results showed that there is considerable intrinsic initial variation among inoculated cysts in their production of buds and that increasing parasite density (crowding) stimulates the overall production of buds and recruit into budding most of the cysts.Identical cultures were then subjected to various treatments such as heating and exposure to peroxide to induce stress, or to 17beta-estradiol, insulin, glucose, or insulin+glucose to supplement putatively limiting hormonal and energy resources.All treatments increased budding but the parasites' strong budding response to crowding alone overshadows the other treatments.

View Article: PubMed Central - PubMed

Affiliation: Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, A.P. 70228, C.P. 04510, México D.F., Mexico. postoa@unam.mx

ABSTRACT
Taenia crassiceps cysticerci (cysts) reproduce by budding. The cysts' production of buds was measured in vitro to explore parasite and environmental-related factors involved in the extreme individual variation in parasite loads of inbred mice. Cysts were placed in in vitro culture for 10 days at initial parasite densities of 1, 5, 10 cysts/well in 1 ml of RPMI Medium 1640 without serum. Results showed that there is considerable intrinsic initial variation among inoculated cysts in their production of buds and that increasing parasite density (crowding) stimulates the overall production of buds and recruit into budding most of the cysts. Identical cultures were then subjected to various treatments such as heating and exposure to peroxide to induce stress, or to 17beta-estradiol, insulin, glucose, or insulin+glucose to supplement putatively limiting hormonal and energy resources. All treatments increased budding but the parasites' strong budding response to crowding alone overshadows the other treatments.

Show MeSH
Related in: MedlinePlus