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Influence of melatonin on cerebrovascular proinflammatory mediators expression and oxidative stress following subarachnoid hemorrhage in rabbits.

Fang Q, Chen G, Zhu W, Dong W, Wang Z - Mediators Inflamm. (2010)

Bottom Line: In animals given melatonin, basilar arterial NF-kappaB and pro-inflammatory cytokines were decreased in comparison to vehicle-treated animals.Measures of oxidative stress also showed significant downregulation after melatonin treatment.In conclusion, post-SAH melatonin administration may attenuate inflammatory response and oxidative stress in the spasmodic artery, and this may be one mechanism involved in the therapeutic effect of melatonin on the subsequent vasospasm after SAH.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, The First Affiliated Hospital of Soochow University, Suzhou 215006, China.

ABSTRACT
The aim of this study is to analyze whether melatonin administration influenced the nuclear factor-kappa B (NF-kappaB) activity, proinflammatory cytokines expression, and oxidative response in the basilar artery after SAH. A total of 48 rabbits were randomly divided into four groups: control group, SAH group, SAH + vehicle group, and SAH + melatonin group. All SAH animals were subjected to injection of autologous blood into cisterna magna twice on day 0 and day 2. The melatonin was administered intraperitoneally at a dose of 5 mg/kg/12 h simultaneously with SAH from day 0 to day 5. The basilar arteries were extracted on day 5 after SAH. As a result, we found that vascular inflammation and oxidative stress were induced in all SAH animals. In animals given melatonin, basilar arterial NF-kappaB and pro-inflammatory cytokines were decreased in comparison to vehicle-treated animals. Measures of oxidative stress also showed significant downregulation after melatonin treatment. Furthermore, administration of melatonin prevented vasospasm on day 5 following SAH. In conclusion, post-SAH melatonin administration may attenuate inflammatory response and oxidative stress in the spasmodic artery, and this may be one mechanism involved in the therapeutic effect of melatonin on the subsequent vasospasm after SAH.

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Changes in the cross-sectional area of basilar arteries in the experimental SAH model. (a)–(d) Representative images of cross-sectional areas of the basilar arteries of the control rabbits or rabbits subjected to SAH alone or SAH plus injection with vehicle or melatonin. Severe vasospasm could be detected in the SAH group, which was attenuated in the SAH + melatonin group. (a) The control group; (b) the SAH group; (c) the SAH + vehicle group; (d) the SAH + melatonin group; (e): Histogram of the average cross-sectional area of the basilar arteries from different groups. There is a significant difference in the basilar artery cross-sectional area between the SAH and control groups. The basilar artery cross-sectional area was significantly increased in the SAH + melatonin group compared with the SAH or SAH + vehicle groups. Results are represented as means ± SD of six rabbits in each group. **P < .01 versus control group, ns P > .05 versus SAH group; ##P < .05 versus SAH + vehicle group.
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fig1: Changes in the cross-sectional area of basilar arteries in the experimental SAH model. (a)–(d) Representative images of cross-sectional areas of the basilar arteries of the control rabbits or rabbits subjected to SAH alone or SAH plus injection with vehicle or melatonin. Severe vasospasm could be detected in the SAH group, which was attenuated in the SAH + melatonin group. (a) The control group; (b) the SAH group; (c) the SAH + vehicle group; (d) the SAH + melatonin group; (e): Histogram of the average cross-sectional area of the basilar arteries from different groups. There is a significant difference in the basilar artery cross-sectional area between the SAH and control groups. The basilar artery cross-sectional area was significantly increased in the SAH + melatonin group compared with the SAH or SAH + vehicle groups. Results are represented as means ± SD of six rabbits in each group. **P < .01 versus control group, ns P > .05 versus SAH group; ##P < .05 versus SAH + vehicle group.

Mentions: As shown in Figure 1, there was a significant difference in the cross-sectional area of basilar artery among all the groups on day 5 following SAH (P < .01). A significant difference was detected between the SAH (211745.2 ± 19158.4 μm2) and the control (416253.4 ± 32508.4 μm2) groups (P < .01) (Figure 1). There was also a significant difference in the basilar arterial cross-sectional area between the SAH + melatonin (372806.5 ± 31195.3 μm2) and SAH + vehicle (213345.6 ± 12213.5 μm2) groups (P < .01) (Figure 1). No significant difference was seen between the SAH group and the SAH + vehicle group (P > .05).


Influence of melatonin on cerebrovascular proinflammatory mediators expression and oxidative stress following subarachnoid hemorrhage in rabbits.

Fang Q, Chen G, Zhu W, Dong W, Wang Z - Mediators Inflamm. (2010)

Changes in the cross-sectional area of basilar arteries in the experimental SAH model. (a)–(d) Representative images of cross-sectional areas of the basilar arteries of the control rabbits or rabbits subjected to SAH alone or SAH plus injection with vehicle or melatonin. Severe vasospasm could be detected in the SAH group, which was attenuated in the SAH + melatonin group. (a) The control group; (b) the SAH group; (c) the SAH + vehicle group; (d) the SAH + melatonin group; (e): Histogram of the average cross-sectional area of the basilar arteries from different groups. There is a significant difference in the basilar artery cross-sectional area between the SAH and control groups. The basilar artery cross-sectional area was significantly increased in the SAH + melatonin group compared with the SAH or SAH + vehicle groups. Results are represented as means ± SD of six rabbits in each group. **P < .01 versus control group, ns P > .05 versus SAH group; ##P < .05 versus SAH + vehicle group.
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC2821769&req=5

fig1: Changes in the cross-sectional area of basilar arteries in the experimental SAH model. (a)–(d) Representative images of cross-sectional areas of the basilar arteries of the control rabbits or rabbits subjected to SAH alone or SAH plus injection with vehicle or melatonin. Severe vasospasm could be detected in the SAH group, which was attenuated in the SAH + melatonin group. (a) The control group; (b) the SAH group; (c) the SAH + vehicle group; (d) the SAH + melatonin group; (e): Histogram of the average cross-sectional area of the basilar arteries from different groups. There is a significant difference in the basilar artery cross-sectional area between the SAH and control groups. The basilar artery cross-sectional area was significantly increased in the SAH + melatonin group compared with the SAH or SAH + vehicle groups. Results are represented as means ± SD of six rabbits in each group. **P < .01 versus control group, ns P > .05 versus SAH group; ##P < .05 versus SAH + vehicle group.
Mentions: As shown in Figure 1, there was a significant difference in the cross-sectional area of basilar artery among all the groups on day 5 following SAH (P < .01). A significant difference was detected between the SAH (211745.2 ± 19158.4 μm2) and the control (416253.4 ± 32508.4 μm2) groups (P < .01) (Figure 1). There was also a significant difference in the basilar arterial cross-sectional area between the SAH + melatonin (372806.5 ± 31195.3 μm2) and SAH + vehicle (213345.6 ± 12213.5 μm2) groups (P < .01) (Figure 1). No significant difference was seen between the SAH group and the SAH + vehicle group (P > .05).

Bottom Line: In animals given melatonin, basilar arterial NF-kappaB and pro-inflammatory cytokines were decreased in comparison to vehicle-treated animals.Measures of oxidative stress also showed significant downregulation after melatonin treatment.In conclusion, post-SAH melatonin administration may attenuate inflammatory response and oxidative stress in the spasmodic artery, and this may be one mechanism involved in the therapeutic effect of melatonin on the subsequent vasospasm after SAH.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, The First Affiliated Hospital of Soochow University, Suzhou 215006, China.

ABSTRACT
The aim of this study is to analyze whether melatonin administration influenced the nuclear factor-kappa B (NF-kappaB) activity, proinflammatory cytokines expression, and oxidative response in the basilar artery after SAH. A total of 48 rabbits were randomly divided into four groups: control group, SAH group, SAH + vehicle group, and SAH + melatonin group. All SAH animals were subjected to injection of autologous blood into cisterna magna twice on day 0 and day 2. The melatonin was administered intraperitoneally at a dose of 5 mg/kg/12 h simultaneously with SAH from day 0 to day 5. The basilar arteries were extracted on day 5 after SAH. As a result, we found that vascular inflammation and oxidative stress were induced in all SAH animals. In animals given melatonin, basilar arterial NF-kappaB and pro-inflammatory cytokines were decreased in comparison to vehicle-treated animals. Measures of oxidative stress also showed significant downregulation after melatonin treatment. Furthermore, administration of melatonin prevented vasospasm on day 5 following SAH. In conclusion, post-SAH melatonin administration may attenuate inflammatory response and oxidative stress in the spasmodic artery, and this may be one mechanism involved in the therapeutic effect of melatonin on the subsequent vasospasm after SAH.

Show MeSH
Related in: MedlinePlus