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DSC Study of Collagen in Disc Disease.

Skrzyński S, Sionkowska A, Marciniak A - J Biophys (2010)

Bottom Line: The heating rate has an influence on the position on denaturation temperatures of collagen in disc tissues.Higher helix-coil transition temperature of collagen in degenerated disc suggests that additional intermolecular cross linking of collagen fibers occurs.Denaturation temperatures of collagen in degenerated male disc possess smaller values than in female ones.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurosurgery, Military Institute of the Health Services, Central Clinical Hospital of the Department of National Defence, Szaserów 128, 00-909 Warsaw, Poland.

ABSTRACT
Differential scanning calorimetry (DSC) has been used to estimate the effect of disc disease on the collagen helix-coil transition and morphology for tissue extracted from patients during surgical operation. Forty discs were obtained from patients with degenerative disc disease undergoing surgery for low back pain. The patients were in the age between 20 and 70 years old. The specimens were kept wet during DSC experiment. The data allow the comparison between thermal stability of collagen tissue from healthy patients and from patients suffering from disc disease. In the paper the comparison between thermal helix-coil transition for collagen fibers from patients suffering from disc disease and collagen fibers from healthy organisms has been discussed. The heating rate has an influence on the position on denaturation temperatures of collagen in disc tissues. Higher helix-coil transition temperature of collagen in degenerated disc suggests that additional intermolecular cross linking of collagen fibers occurs. Denaturation temperatures of collagen in degenerated male disc possess smaller values than in female ones. Disc disease induces changes in collagen structure and leads to formation of additional crosslinks between collagen fibers.

No MeSH data available.


Related in: MedlinePlus

The example of XRD measurement of degenerated disc.
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Related In: Results  -  Collection


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fig4: The example of XRD measurement of degenerated disc.

Mentions: We employed the X-ray diffraction (XRD) analysis to find out whether the crystal domains are present in disc tissue. The examples of XRD spectra are presented in Figure 4. XRD results confirmed the presence of crystal and amorphous phase in collagen. However, we could not find a dependence between the amount of crystal and amorphous phase in collagen and the age of patients nor the gender.


DSC Study of Collagen in Disc Disease.

Skrzyński S, Sionkowska A, Marciniak A - J Biophys (2010)

The example of XRD measurement of degenerated disc.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2821760&req=5

fig4: The example of XRD measurement of degenerated disc.
Mentions: We employed the X-ray diffraction (XRD) analysis to find out whether the crystal domains are present in disc tissue. The examples of XRD spectra are presented in Figure 4. XRD results confirmed the presence of crystal and amorphous phase in collagen. However, we could not find a dependence between the amount of crystal and amorphous phase in collagen and the age of patients nor the gender.

Bottom Line: The heating rate has an influence on the position on denaturation temperatures of collagen in disc tissues.Higher helix-coil transition temperature of collagen in degenerated disc suggests that additional intermolecular cross linking of collagen fibers occurs.Denaturation temperatures of collagen in degenerated male disc possess smaller values than in female ones.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurosurgery, Military Institute of the Health Services, Central Clinical Hospital of the Department of National Defence, Szaserów 128, 00-909 Warsaw, Poland.

ABSTRACT
Differential scanning calorimetry (DSC) has been used to estimate the effect of disc disease on the collagen helix-coil transition and morphology for tissue extracted from patients during surgical operation. Forty discs were obtained from patients with degenerative disc disease undergoing surgery for low back pain. The patients were in the age between 20 and 70 years old. The specimens were kept wet during DSC experiment. The data allow the comparison between thermal stability of collagen tissue from healthy patients and from patients suffering from disc disease. In the paper the comparison between thermal helix-coil transition for collagen fibers from patients suffering from disc disease and collagen fibers from healthy organisms has been discussed. The heating rate has an influence on the position on denaturation temperatures of collagen in disc tissues. Higher helix-coil transition temperature of collagen in degenerated disc suggests that additional intermolecular cross linking of collagen fibers occurs. Denaturation temperatures of collagen in degenerated male disc possess smaller values than in female ones. Disc disease induces changes in collagen structure and leads to formation of additional crosslinks between collagen fibers.

No MeSH data available.


Related in: MedlinePlus