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DSC Study of Collagen in Disc Disease.

Skrzyński S, Sionkowska A, Marciniak A - J Biophys (2010)

Bottom Line: The heating rate has an influence on the position on denaturation temperatures of collagen in disc tissues.Higher helix-coil transition temperature of collagen in degenerated disc suggests that additional intermolecular cross linking of collagen fibers occurs.Denaturation temperatures of collagen in degenerated male disc possess smaller values than in female ones.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurosurgery, Military Institute of the Health Services, Central Clinical Hospital of the Department of National Defence, Szaserów 128, 00-909 Warsaw, Poland.

ABSTRACT
Differential scanning calorimetry (DSC) has been used to estimate the effect of disc disease on the collagen helix-coil transition and morphology for tissue extracted from patients during surgical operation. Forty discs were obtained from patients with degenerative disc disease undergoing surgery for low back pain. The patients were in the age between 20 and 70 years old. The specimens were kept wet during DSC experiment. The data allow the comparison between thermal stability of collagen tissue from healthy patients and from patients suffering from disc disease. In the paper the comparison between thermal helix-coil transition for collagen fibers from patients suffering from disc disease and collagen fibers from healthy organisms has been discussed. The heating rate has an influence on the position on denaturation temperatures of collagen in disc tissues. Higher helix-coil transition temperature of collagen in degenerated disc suggests that additional intermolecular cross linking of collagen fibers occurs. Denaturation temperatures of collagen in degenerated male disc possess smaller values than in female ones. Disc disease induces changes in collagen structure and leads to formation of additional crosslinks between collagen fibers.

No MeSH data available.


Related in: MedlinePlus

DSC traces of healthy and degenerated disc samples heated with rate of 5°C per minute. (blue line—healthy: women, 36 years old; red line—degenerated disc from  woman, 37 years old).
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fig2: DSC traces of healthy and degenerated disc samples heated with rate of 5°C per minute. (blue line—healthy: women, 36 years old; red line—degenerated disc from woman, 37 years old).

Mentions: DSC curves for collagen in healthy disc and collagen in disc degenerated by discopathy are presented in Figures 1 and 2. The curves in Figure 1 have been obtained with heating rate of 0.5°C/min. The curves in Figure 2 have been obtained with heating rate of 5°C/min. As can be seen the heating rate has an influence on the position of the peak of denaturation temperatures of collagen in disc tissues. The helix-coil transition for collagen from healthy disc appears near 94.5°C (when DSC curves were recorded with heating rate of 5°C/min) and near 50°C (when DSC curves were recorded with heating rate of 0.5°C/min). For collagen in degenerated disc, DSC peak appears in the range of 84–104°C (when DSC curves were recorded with heating rate of 5°C/min) and in the range of 51-52°C (when DSC curves were recorded with heating rate of 0.5°C/min). The data are listed in Tables 1 and 2. DSC profiles show that disc disease induced changes in molecular structure of collagen in disc tissue that alters position in DSC peak of collagen (highly energetic and sharp denaturation endotherm that is a characteristic of the triple helix). Higher helix-coil transition temperature in degenerated disc collagen suggests that additional intermolecular cross-linking of collagen fibers occurs. As a healthy disc came from female disc, we compare DSC curves for healthy and degenerated only for female disc (we did not have male healthy disc). However, we compared DSC curves of female degenerated discs and male degenerated discs. We observed that the maximum of peak responsible for thermal denaturation of collagen in male disc is in the range of 80.95–97.87°C, whereas for female it is in the range of 86.25–104.47°C (Figure 3). It may suggest that collagen in female disc possesses more cross-linking linkages than collagen in male discs.


DSC Study of Collagen in Disc Disease.

Skrzyński S, Sionkowska A, Marciniak A - J Biophys (2010)

DSC traces of healthy and degenerated disc samples heated with rate of 5°C per minute. (blue line—healthy: women, 36 years old; red line—degenerated disc from  woman, 37 years old).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2821760&req=5

fig2: DSC traces of healthy and degenerated disc samples heated with rate of 5°C per minute. (blue line—healthy: women, 36 years old; red line—degenerated disc from woman, 37 years old).
Mentions: DSC curves for collagen in healthy disc and collagen in disc degenerated by discopathy are presented in Figures 1 and 2. The curves in Figure 1 have been obtained with heating rate of 0.5°C/min. The curves in Figure 2 have been obtained with heating rate of 5°C/min. As can be seen the heating rate has an influence on the position of the peak of denaturation temperatures of collagen in disc tissues. The helix-coil transition for collagen from healthy disc appears near 94.5°C (when DSC curves were recorded with heating rate of 5°C/min) and near 50°C (when DSC curves were recorded with heating rate of 0.5°C/min). For collagen in degenerated disc, DSC peak appears in the range of 84–104°C (when DSC curves were recorded with heating rate of 5°C/min) and in the range of 51-52°C (when DSC curves were recorded with heating rate of 0.5°C/min). The data are listed in Tables 1 and 2. DSC profiles show that disc disease induced changes in molecular structure of collagen in disc tissue that alters position in DSC peak of collagen (highly energetic and sharp denaturation endotherm that is a characteristic of the triple helix). Higher helix-coil transition temperature in degenerated disc collagen suggests that additional intermolecular cross-linking of collagen fibers occurs. As a healthy disc came from female disc, we compare DSC curves for healthy and degenerated only for female disc (we did not have male healthy disc). However, we compared DSC curves of female degenerated discs and male degenerated discs. We observed that the maximum of peak responsible for thermal denaturation of collagen in male disc is in the range of 80.95–97.87°C, whereas for female it is in the range of 86.25–104.47°C (Figure 3). It may suggest that collagen in female disc possesses more cross-linking linkages than collagen in male discs.

Bottom Line: The heating rate has an influence on the position on denaturation temperatures of collagen in disc tissues.Higher helix-coil transition temperature of collagen in degenerated disc suggests that additional intermolecular cross linking of collagen fibers occurs.Denaturation temperatures of collagen in degenerated male disc possess smaller values than in female ones.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurosurgery, Military Institute of the Health Services, Central Clinical Hospital of the Department of National Defence, Szaserów 128, 00-909 Warsaw, Poland.

ABSTRACT
Differential scanning calorimetry (DSC) has been used to estimate the effect of disc disease on the collagen helix-coil transition and morphology for tissue extracted from patients during surgical operation. Forty discs were obtained from patients with degenerative disc disease undergoing surgery for low back pain. The patients were in the age between 20 and 70 years old. The specimens were kept wet during DSC experiment. The data allow the comparison between thermal stability of collagen tissue from healthy patients and from patients suffering from disc disease. In the paper the comparison between thermal helix-coil transition for collagen fibers from patients suffering from disc disease and collagen fibers from healthy organisms has been discussed. The heating rate has an influence on the position on denaturation temperatures of collagen in disc tissues. Higher helix-coil transition temperature of collagen in degenerated disc suggests that additional intermolecular cross linking of collagen fibers occurs. Denaturation temperatures of collagen in degenerated male disc possess smaller values than in female ones. Disc disease induces changes in collagen structure and leads to formation of additional crosslinks between collagen fibers.

No MeSH data available.


Related in: MedlinePlus