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Experimental vaccination of sheep and cattle against tick infestation using recombinant 5'-nucleotidase.

Hope M, Jiang X, Gough J, Cadogan L, Josh P, Jonsson N, Willadsen P - Parasite Immunol. (2010)

Bottom Line: Although Bm86 showed typical efficacy, no significant protection was seen in cattle vaccinated with 5'-nucleotidase.Cattle receiving a dual antigen formulation were no better protected than those receiving Bm86 alone.This demonstrates a limitation of using a model host like sheep in vaccine studies.

View Article: PubMed Central - PubMed

Affiliation: CSIRO Livestock Industries, St Lucia, Queensland, Australia.

ABSTRACT
Limited prior evidence suggests that 5'-nucleotidase, an ectoenzyme principally located in the Malpighian tubules of the tick Rhipicephalus (Boophilus) microplus, could be an effective antigen in an anti-tick vaccine. To assess this, recombinant 5'-nucleotidase was expressed in Escherichia coli and used in vaccination trials with both sheep and cattle. Vaccinated sheep were challenged with freshly moulted adult ticks. Those with high titres of anti-nucleotidase antibodies showed significant protection against tick infestation, although protection was less than that found with the previously characterized antigen, Bm86. Cattle were vaccinated, in separate groups, with 5'-nucleotidase, Bm86 and both antigens combined. Cattle, as the natural host, were challenged with larval ticks. Although Bm86 showed typical efficacy, no significant protection was seen in cattle vaccinated with 5'-nucleotidase. Cattle receiving a dual antigen formulation were no better protected than those receiving Bm86 alone. One possible reason for the difference between host species, namely antibody titre, was examined and shown to be an unlikely explanation. This demonstrates a limitation of using a model host like sheep in vaccine studies.

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Related in: MedlinePlus

Log titres bovine antibody to 5′-nucleotidase, measured as described in Materials and methods, in the presence of varying dilutions of ovine anti-5′-nucleotidase. The detection reagent was ovine anti-bovine IgG antibody coupled to peroxidase. The log titre in the absence of any ovine antiserum was 4·7.
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fig03: Log titres bovine antibody to 5′-nucleotidase, measured as described in Materials and methods, in the presence of varying dilutions of ovine anti-5′-nucleotidase. The detection reagent was ovine anti-bovine IgG antibody coupled to peroxidase. The log titre in the absence of any ovine antiserum was 4·7.

Mentions: In a second experimental approach, serial dilutions of bovine anti-nucleotidase serum were made in the presence of dilutions of ovine anti-nucleotidase ranging from 1/2000 to 1/64 000. Normal ELISAs were then performed with these dilutions, using as a second antibody peroxidase-coupled ovine anti-bovine IgG. This therefore measured the ability of an excess of ovine anti-nucleotidase to compete with bovine antibody for bound nucleotidase. As expected, there was no reaction of the second antibody with ovine IgG. The results are shown in Figure 3 and demonstrate that the competition of the ovine anti-nucleotidase with the bovine equivalent was even less than expected. For example, a 1/2000 dilution of ovine antibody reduced the binding of a 1/50 000 dilution of the bovine antibody by only 12%.


Experimental vaccination of sheep and cattle against tick infestation using recombinant 5'-nucleotidase.

Hope M, Jiang X, Gough J, Cadogan L, Josh P, Jonsson N, Willadsen P - Parasite Immunol. (2010)

Log titres bovine antibody to 5′-nucleotidase, measured as described in Materials and methods, in the presence of varying dilutions of ovine anti-5′-nucleotidase. The detection reagent was ovine anti-bovine IgG antibody coupled to peroxidase. The log titre in the absence of any ovine antiserum was 4·7.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2821529&req=5

fig03: Log titres bovine antibody to 5′-nucleotidase, measured as described in Materials and methods, in the presence of varying dilutions of ovine anti-5′-nucleotidase. The detection reagent was ovine anti-bovine IgG antibody coupled to peroxidase. The log titre in the absence of any ovine antiserum was 4·7.
Mentions: In a second experimental approach, serial dilutions of bovine anti-nucleotidase serum were made in the presence of dilutions of ovine anti-nucleotidase ranging from 1/2000 to 1/64 000. Normal ELISAs were then performed with these dilutions, using as a second antibody peroxidase-coupled ovine anti-bovine IgG. This therefore measured the ability of an excess of ovine anti-nucleotidase to compete with bovine antibody for bound nucleotidase. As expected, there was no reaction of the second antibody with ovine IgG. The results are shown in Figure 3 and demonstrate that the competition of the ovine anti-nucleotidase with the bovine equivalent was even less than expected. For example, a 1/2000 dilution of ovine antibody reduced the binding of a 1/50 000 dilution of the bovine antibody by only 12%.

Bottom Line: Although Bm86 showed typical efficacy, no significant protection was seen in cattle vaccinated with 5'-nucleotidase.Cattle receiving a dual antigen formulation were no better protected than those receiving Bm86 alone.This demonstrates a limitation of using a model host like sheep in vaccine studies.

View Article: PubMed Central - PubMed

Affiliation: CSIRO Livestock Industries, St Lucia, Queensland, Australia.

ABSTRACT
Limited prior evidence suggests that 5'-nucleotidase, an ectoenzyme principally located in the Malpighian tubules of the tick Rhipicephalus (Boophilus) microplus, could be an effective antigen in an anti-tick vaccine. To assess this, recombinant 5'-nucleotidase was expressed in Escherichia coli and used in vaccination trials with both sheep and cattle. Vaccinated sheep were challenged with freshly moulted adult ticks. Those with high titres of anti-nucleotidase antibodies showed significant protection against tick infestation, although protection was less than that found with the previously characterized antigen, Bm86. Cattle were vaccinated, in separate groups, with 5'-nucleotidase, Bm86 and both antigens combined. Cattle, as the natural host, were challenged with larval ticks. Although Bm86 showed typical efficacy, no significant protection was seen in cattle vaccinated with 5'-nucleotidase. Cattle receiving a dual antigen formulation were no better protected than those receiving Bm86 alone. One possible reason for the difference between host species, namely antibody titre, was examined and shown to be an unlikely explanation. This demonstrates a limitation of using a model host like sheep in vaccine studies.

Show MeSH
Related in: MedlinePlus