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A fluorescent probe for diacetyl detection.

Li X, Duerkop A, Wolfbeis OS - J Fluoresc (2008)

Bottom Line: A water-soluble fluorescent probe, rhodamine B hydrazide (RBH), was prepared and its properties for recognition of diacetyl were studied.The method employs the reaction of diacetyl with RBH, a colorless and non-fluorescent rhodamine B spiro form derivative to give a pink-colored fluorescent substance.In weakly acidic media, RBH reacts more selectively with diacetyl than with other carbonyls, causing a large increase in fluorescence intensity and thereby providing an easy assay for the determination of diacetyl.

View Article: PubMed Central - PubMed

Affiliation: Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, 93040 Regensburg, Germany.

ABSTRACT
A water-soluble fluorescent probe, rhodamine B hydrazide (RBH), was prepared and its properties for recognition of diacetyl were studied. The method employs the reaction of diacetyl with RBH, a colorless and non-fluorescent rhodamine B spiro form derivative to give a pink-colored fluorescent substance. In weakly acidic media, RBH reacts more selectively with diacetyl than with other carbonyls, causing a large increase in fluorescence intensity and thereby providing an easy assay for the determination of diacetyl.

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Absorption spectra of RBH before (solid line) and after (dashed line) reaction with diacetyl. Conditions: 200 μmol/L of RBH, 100 μmol/L of diacetyl; reacted in pH 3 citrate-phosphate buffer (50 mmol/L) at 37 °C for 3 h
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Fig4: Absorption spectra of RBH before (solid line) and after (dashed line) reaction with diacetyl. Conditions: 200 μmol/L of RBH, 100 μmol/L of diacetyl; reacted in pH 3 citrate-phosphate buffer (50 mmol/L) at 37 °C for 3 h

Mentions: The absorption spectra of RBH (200 μmol/L) before and after the reaction with 100 μmol/L of diacetyl at 37 °C in pH 3 citrate–phosphate buffer solution (50 mmol/L) are shown in Fig. 4. In accordance with the change of fluorescence, the absorbance also increases to almost the same degree after reaction with diacetyl. The absorption maximum only slightly changes from 559 nm to 562 nm. Obviously, the reaction of diacetyl with RBH causes a structural change in the molecule, as shown in Scheme 2.Fig. 4


A fluorescent probe for diacetyl detection.

Li X, Duerkop A, Wolfbeis OS - J Fluoresc (2008)

Absorption spectra of RBH before (solid line) and after (dashed line) reaction with diacetyl. Conditions: 200 μmol/L of RBH, 100 μmol/L of diacetyl; reacted in pH 3 citrate-phosphate buffer (50 mmol/L) at 37 °C for 3 h
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Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2821512&req=5

Fig4: Absorption spectra of RBH before (solid line) and after (dashed line) reaction with diacetyl. Conditions: 200 μmol/L of RBH, 100 μmol/L of diacetyl; reacted in pH 3 citrate-phosphate buffer (50 mmol/L) at 37 °C for 3 h
Mentions: The absorption spectra of RBH (200 μmol/L) before and after the reaction with 100 μmol/L of diacetyl at 37 °C in pH 3 citrate–phosphate buffer solution (50 mmol/L) are shown in Fig. 4. In accordance with the change of fluorescence, the absorbance also increases to almost the same degree after reaction with diacetyl. The absorption maximum only slightly changes from 559 nm to 562 nm. Obviously, the reaction of diacetyl with RBH causes a structural change in the molecule, as shown in Scheme 2.Fig. 4

Bottom Line: A water-soluble fluorescent probe, rhodamine B hydrazide (RBH), was prepared and its properties for recognition of diacetyl were studied.The method employs the reaction of diacetyl with RBH, a colorless and non-fluorescent rhodamine B spiro form derivative to give a pink-colored fluorescent substance.In weakly acidic media, RBH reacts more selectively with diacetyl than with other carbonyls, causing a large increase in fluorescence intensity and thereby providing an easy assay for the determination of diacetyl.

View Article: PubMed Central - PubMed

Affiliation: Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, 93040 Regensburg, Germany.

ABSTRACT
A water-soluble fluorescent probe, rhodamine B hydrazide (RBH), was prepared and its properties for recognition of diacetyl were studied. The method employs the reaction of diacetyl with RBH, a colorless and non-fluorescent rhodamine B spiro form derivative to give a pink-colored fluorescent substance. In weakly acidic media, RBH reacts more selectively with diacetyl than with other carbonyls, causing a large increase in fluorescence intensity and thereby providing an easy assay for the determination of diacetyl.

Show MeSH