Limits...
A fluorescent probe for diacetyl detection.

Li X, Duerkop A, Wolfbeis OS - J Fluoresc (2008)

Bottom Line: A water-soluble fluorescent probe, rhodamine B hydrazide (RBH), was prepared and its properties for recognition of diacetyl were studied.The method employs the reaction of diacetyl with RBH, a colorless and non-fluorescent rhodamine B spiro form derivative to give a pink-colored fluorescent substance.In weakly acidic media, RBH reacts more selectively with diacetyl than with other carbonyls, causing a large increase in fluorescence intensity and thereby providing an easy assay for the determination of diacetyl.

View Article: PubMed Central - PubMed

Affiliation: Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, 93040 Regensburg, Germany.

ABSTRACT
A water-soluble fluorescent probe, rhodamine B hydrazide (RBH), was prepared and its properties for recognition of diacetyl were studied. The method employs the reaction of diacetyl with RBH, a colorless and non-fluorescent rhodamine B spiro form derivative to give a pink-colored fluorescent substance. In weakly acidic media, RBH reacts more selectively with diacetyl than with other carbonyls, causing a large increase in fluorescence intensity and thereby providing an easy assay for the determination of diacetyl.

Show MeSH
Effect of reaction time on the reaction of RBH with diacetyl. Conditions: 200 μmol/L of RBH, 100 μmol/L of diacetyl, reacted at 37 °C in pH 3 citrate-phosphate buffer solution (50 mmol/L)
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC2821512&req=5

Fig3: Effect of reaction time on the reaction of RBH with diacetyl. Conditions: 200 μmol/L of RBH, 100 μmol/L of diacetyl, reacted at 37 °C in pH 3 citrate-phosphate buffer solution (50 mmol/L)

Mentions: The reaction rate of 200 μmol/L of RBH with 100 μmol/L of diacetyl was also examined at 37 °C and pH 3. Figure 3 shows that after 2.5 h incubation the derivatization is completed. Due to the pronounced increase of fluorescence intensity, one could also use a kinetic determination after 1.5 h, where more than half of the total fluorescence increase has already appeared.Fig. 3


A fluorescent probe for diacetyl detection.

Li X, Duerkop A, Wolfbeis OS - J Fluoresc (2008)

Effect of reaction time on the reaction of RBH with diacetyl. Conditions: 200 μmol/L of RBH, 100 μmol/L of diacetyl, reacted at 37 °C in pH 3 citrate-phosphate buffer solution (50 mmol/L)
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2821512&req=5

Fig3: Effect of reaction time on the reaction of RBH with diacetyl. Conditions: 200 μmol/L of RBH, 100 μmol/L of diacetyl, reacted at 37 °C in pH 3 citrate-phosphate buffer solution (50 mmol/L)
Mentions: The reaction rate of 200 μmol/L of RBH with 100 μmol/L of diacetyl was also examined at 37 °C and pH 3. Figure 3 shows that after 2.5 h incubation the derivatization is completed. Due to the pronounced increase of fluorescence intensity, one could also use a kinetic determination after 1.5 h, where more than half of the total fluorescence increase has already appeared.Fig. 3

Bottom Line: A water-soluble fluorescent probe, rhodamine B hydrazide (RBH), was prepared and its properties for recognition of diacetyl were studied.The method employs the reaction of diacetyl with RBH, a colorless and non-fluorescent rhodamine B spiro form derivative to give a pink-colored fluorescent substance.In weakly acidic media, RBH reacts more selectively with diacetyl than with other carbonyls, causing a large increase in fluorescence intensity and thereby providing an easy assay for the determination of diacetyl.

View Article: PubMed Central - PubMed

Affiliation: Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, 93040 Regensburg, Germany.

ABSTRACT
A water-soluble fluorescent probe, rhodamine B hydrazide (RBH), was prepared and its properties for recognition of diacetyl were studied. The method employs the reaction of diacetyl with RBH, a colorless and non-fluorescent rhodamine B spiro form derivative to give a pink-colored fluorescent substance. In weakly acidic media, RBH reacts more selectively with diacetyl than with other carbonyls, causing a large increase in fluorescence intensity and thereby providing an easy assay for the determination of diacetyl.

Show MeSH