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Immunogenicity of antigens from the TbD1 region present in M. africanum and missing from "modern" M. tuberculosis: a cross- sectional study.

de Jong BC, Hammond A, Otu JK, Antonio M, Adegbola RA, Ota MO - BMC Infect. Dis. (2010)

Bottom Line: M. africanum causes up to half of TB in West- Africa and its relatively lower progression to disease suggests the presence of a large reservoir of latent infection relative to M. tuberculosis.Antigens from the TbD1 region induced IFNgamma responses in only 35% patients and did not discriminate between patients infected with M. africanum vs.Further studies will need to assess other antigens unique to M. africanum that may induce discriminatory immune responses.

View Article: PubMed Central - HTML - PubMed

Affiliation: Bacterial Diseases Programme, MRC Laboratories, POB 273, Banjul, the Gambia. bdejong@mrc.gm

ABSTRACT

Background: Currently available tools cannot be used to distinguish between sub-species of the M. tuberculosis complex causing latent tuberculosis (TB) infection. M. africanum causes up to half of TB in West- Africa and its relatively lower progression to disease suggests the presence of a large reservoir of latent infection relative to M. tuberculosis.

Methods: We assessed the immunogenicity of the TbD1 region, present in M. africanum and absent from "modern" M. tuberculosis, in an ELISPOT assay using cells from confirmed M. africanum or M. tuberculosis infected TB patients without HIV infection in the Gambia.

Results: Antigens from the TbD1 region induced IFNgamma responses in only 35% patients and did not discriminate between patients infected with M. africanum vs. M. tuberculosis, while PPD induced universally high responses.

Conclusions: Further studies will need to assess other antigens unique to M. africanum that may induce discriminatory immune responses.

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Differences in ELISPOT responses between TB casesinfected with M. tuberculosis versus M. africanum. The TbD1 based antigens mmpl6C, mmpl6N, and mmps6 did not induce robust responses in M. africanum infected patients, despite the presence of an intact TbD1 region in M. africanum. The horizontal lines indicate the mean and SEM. FP = ESAT-6/CFP-10 fusion protein.
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Figure 1: Differences in ELISPOT responses between TB casesinfected with M. tuberculosis versus M. africanum. The TbD1 based antigens mmpl6C, mmpl6N, and mmps6 did not induce robust responses in M. africanum infected patients, despite the presence of an intact TbD1 region in M. africanum. The horizontal lines indicate the mean and SEM. FP = ESAT-6/CFP-10 fusion protein.

Mentions: The mmpL6-C peptide induced IFNγ responses in 19% of M. africanum infected cases, the mmpL6-N peptide in 17%, and the mmpS6 peptide in 20%. The proportion who responded to any of the three TbD1 antigens was similar between M. africanum infected patients and M. tuberculosis infected patients (respectively 33% and 37%, p = 0.80). Equal numbers of patients infected with M. africanum and with M. tuberculosis responded to PPD (respectively 95% and 90%, p = 0.49), whereas responses to ESAT-6, CFP-10 and ESAT-6/CFP-10 fusion protein tended to be lower in M. africanum infected patients (Figure 1).


Immunogenicity of antigens from the TbD1 region present in M. africanum and missing from "modern" M. tuberculosis: a cross- sectional study.

de Jong BC, Hammond A, Otu JK, Antonio M, Adegbola RA, Ota MO - BMC Infect. Dis. (2010)

Differences in ELISPOT responses between TB casesinfected with M. tuberculosis versus M. africanum. The TbD1 based antigens mmpl6C, mmpl6N, and mmps6 did not induce robust responses in M. africanum infected patients, despite the presence of an intact TbD1 region in M. africanum. The horizontal lines indicate the mean and SEM. FP = ESAT-6/CFP-10 fusion protein.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2821388&req=5

Figure 1: Differences in ELISPOT responses between TB casesinfected with M. tuberculosis versus M. africanum. The TbD1 based antigens mmpl6C, mmpl6N, and mmps6 did not induce robust responses in M. africanum infected patients, despite the presence of an intact TbD1 region in M. africanum. The horizontal lines indicate the mean and SEM. FP = ESAT-6/CFP-10 fusion protein.
Mentions: The mmpL6-C peptide induced IFNγ responses in 19% of M. africanum infected cases, the mmpL6-N peptide in 17%, and the mmpS6 peptide in 20%. The proportion who responded to any of the three TbD1 antigens was similar between M. africanum infected patients and M. tuberculosis infected patients (respectively 33% and 37%, p = 0.80). Equal numbers of patients infected with M. africanum and with M. tuberculosis responded to PPD (respectively 95% and 90%, p = 0.49), whereas responses to ESAT-6, CFP-10 and ESAT-6/CFP-10 fusion protein tended to be lower in M. africanum infected patients (Figure 1).

Bottom Line: M. africanum causes up to half of TB in West- Africa and its relatively lower progression to disease suggests the presence of a large reservoir of latent infection relative to M. tuberculosis.Antigens from the TbD1 region induced IFNgamma responses in only 35% patients and did not discriminate between patients infected with M. africanum vs.Further studies will need to assess other antigens unique to M. africanum that may induce discriminatory immune responses.

View Article: PubMed Central - HTML - PubMed

Affiliation: Bacterial Diseases Programme, MRC Laboratories, POB 273, Banjul, the Gambia. bdejong@mrc.gm

ABSTRACT

Background: Currently available tools cannot be used to distinguish between sub-species of the M. tuberculosis complex causing latent tuberculosis (TB) infection. M. africanum causes up to half of TB in West- Africa and its relatively lower progression to disease suggests the presence of a large reservoir of latent infection relative to M. tuberculosis.

Methods: We assessed the immunogenicity of the TbD1 region, present in M. africanum and absent from "modern" M. tuberculosis, in an ELISPOT assay using cells from confirmed M. africanum or M. tuberculosis infected TB patients without HIV infection in the Gambia.

Results: Antigens from the TbD1 region induced IFNgamma responses in only 35% patients and did not discriminate between patients infected with M. africanum vs. M. tuberculosis, while PPD induced universally high responses.

Conclusions: Further studies will need to assess other antigens unique to M. africanum that may induce discriminatory immune responses.

Show MeSH
Related in: MedlinePlus