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Species and age related differences in the type and distribution of influenza virus receptors in different tissues of chickens, ducks and turkeys.

Pillai SP, Lee CW - Virol. J. (2010)

Bottom Line: In contrast, a greater amount of staining for alpha2,3SA-gal (50-80%) and alpha2,6SA-gal (20-50%) receptors were observed along the epithelium of small and large intestine of chickens.Kidney and esophagus sections from the 3 bird species also expressed both avian and human type receptors.In other tissues examined, brain, breast muscles, bursa, spleen, cecal tonsils and oviduct, human type receptors were absent.

View Article: PubMed Central - HTML - PubMed

Affiliation: Food Animal Health Research Program, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, Ohio 44691, USA.

ABSTRACT
We undertook one of the most detailed studies on the distribution of alpha2,3 sialic acid (SA)-galactose (gal) (avian type) and alpha2,6SA-gal (human type) receptors on different tissues of chickens, ducks and turkeys of varying age groups. On the tracheal epithelium, all 3 bird species expressed strong positive staining (80-90%) for alpha2,3SA-gal receptors in the 3 different age groups. In addition, a lesser amount of alpha2,6SA-gal receptors (30-90%) were observed with slight differences in distribution with age and species. The epithelium of the small and large intestine of turkeys and ducks showed negligible staining for alpha2,6SA-gal receptors whereas the large intestine consistently showed 40-70% positive staining for alpha2,3SA-gal receptors. In contrast, a greater amount of staining for alpha2,3SA-gal (50-80%) and alpha2,6SA-gal (20-50%) receptors were observed along the epithelium of small and large intestine of chickens. Kidney and esophagus sections from the 3 bird species also expressed both avian and human type receptors. In other tissues examined, brain, breast muscles, bursa, spleen, cecal tonsils and oviduct, human type receptors were absent. Though different viral and receptor components may play roles in successful viral replication and transmission, understanding the receptor types and distribution in different tissues of domestic birds might be good initial tool to understand host factors that promote successful influenza viral infection.

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Related in: MedlinePlus

Sections of kidney (3.A, B) and esophagus (3.C, D) from 4-week-old chickens stained with MAA and SNA, respectively. Sections of bursa (3.E), brain (3.F), cecal tonsil (3.G) from 4-week-old chickens stained with MAA.
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Figure 3: Sections of kidney (3.A, B) and esophagus (3.C, D) from 4-week-old chickens stained with MAA and SNA, respectively. Sections of bursa (3.E), brain (3.F), cecal tonsil (3.G) from 4-week-old chickens stained with MAA.

Mentions: Similar to the 1-day-old birds, the 2-4-week-old birds and layer birds of the 3 species showed strong staining (++++) in the tubular cells of the kidney (50-60%) for the avian type receptors. The tubular cells also showed positive staining for the human type receptors, although the strained cells was less (10-30%) and mild to moderate intensity (+ to ++) of staining was observed (Fig. 3A and 3B).


Species and age related differences in the type and distribution of influenza virus receptors in different tissues of chickens, ducks and turkeys.

Pillai SP, Lee CW - Virol. J. (2010)

Sections of kidney (3.A, B) and esophagus (3.C, D) from 4-week-old chickens stained with MAA and SNA, respectively. Sections of bursa (3.E), brain (3.F), cecal tonsil (3.G) from 4-week-old chickens stained with MAA.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2821308&req=5

Figure 3: Sections of kidney (3.A, B) and esophagus (3.C, D) from 4-week-old chickens stained with MAA and SNA, respectively. Sections of bursa (3.E), brain (3.F), cecal tonsil (3.G) from 4-week-old chickens stained with MAA.
Mentions: Similar to the 1-day-old birds, the 2-4-week-old birds and layer birds of the 3 species showed strong staining (++++) in the tubular cells of the kidney (50-60%) for the avian type receptors. The tubular cells also showed positive staining for the human type receptors, although the strained cells was less (10-30%) and mild to moderate intensity (+ to ++) of staining was observed (Fig. 3A and 3B).

Bottom Line: In contrast, a greater amount of staining for alpha2,3SA-gal (50-80%) and alpha2,6SA-gal (20-50%) receptors were observed along the epithelium of small and large intestine of chickens.Kidney and esophagus sections from the 3 bird species also expressed both avian and human type receptors.In other tissues examined, brain, breast muscles, bursa, spleen, cecal tonsils and oviduct, human type receptors were absent.

View Article: PubMed Central - HTML - PubMed

Affiliation: Food Animal Health Research Program, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, Ohio 44691, USA.

ABSTRACT
We undertook one of the most detailed studies on the distribution of alpha2,3 sialic acid (SA)-galactose (gal) (avian type) and alpha2,6SA-gal (human type) receptors on different tissues of chickens, ducks and turkeys of varying age groups. On the tracheal epithelium, all 3 bird species expressed strong positive staining (80-90%) for alpha2,3SA-gal receptors in the 3 different age groups. In addition, a lesser amount of alpha2,6SA-gal receptors (30-90%) were observed with slight differences in distribution with age and species. The epithelium of the small and large intestine of turkeys and ducks showed negligible staining for alpha2,6SA-gal receptors whereas the large intestine consistently showed 40-70% positive staining for alpha2,3SA-gal receptors. In contrast, a greater amount of staining for alpha2,3SA-gal (50-80%) and alpha2,6SA-gal (20-50%) receptors were observed along the epithelium of small and large intestine of chickens. Kidney and esophagus sections from the 3 bird species also expressed both avian and human type receptors. In other tissues examined, brain, breast muscles, bursa, spleen, cecal tonsils and oviduct, human type receptors were absent. Though different viral and receptor components may play roles in successful viral replication and transmission, understanding the receptor types and distribution in different tissues of domestic birds might be good initial tool to understand host factors that promote successful influenza viral infection.

Show MeSH
Related in: MedlinePlus