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Species and age related differences in the type and distribution of influenza virus receptors in different tissues of chickens, ducks and turkeys.

Pillai SP, Lee CW - Virol. J. (2010)

Bottom Line: In contrast, a greater amount of staining for alpha2,3SA-gal (50-80%) and alpha2,6SA-gal (20-50%) receptors were observed along the epithelium of small and large intestine of chickens.Kidney and esophagus sections from the 3 bird species also expressed both avian and human type receptors.In other tissues examined, brain, breast muscles, bursa, spleen, cecal tonsils and oviduct, human type receptors were absent.

View Article: PubMed Central - HTML - PubMed

Affiliation: Food Animal Health Research Program, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, Ohio 44691, USA.

ABSTRACT
We undertook one of the most detailed studies on the distribution of alpha2,3 sialic acid (SA)-galactose (gal) (avian type) and alpha2,6SA-gal (human type) receptors on different tissues of chickens, ducks and turkeys of varying age groups. On the tracheal epithelium, all 3 bird species expressed strong positive staining (80-90%) for alpha2,3SA-gal receptors in the 3 different age groups. In addition, a lesser amount of alpha2,6SA-gal receptors (30-90%) were observed with slight differences in distribution with age and species. The epithelium of the small and large intestine of turkeys and ducks showed negligible staining for alpha2,6SA-gal receptors whereas the large intestine consistently showed 40-70% positive staining for alpha2,3SA-gal receptors. In contrast, a greater amount of staining for alpha2,3SA-gal (50-80%) and alpha2,6SA-gal (20-50%) receptors were observed along the epithelium of small and large intestine of chickens. Kidney and esophagus sections from the 3 bird species also expressed both avian and human type receptors. In other tissues examined, brain, breast muscles, bursa, spleen, cecal tonsils and oviduct, human type receptors were absent. Though different viral and receptor components may play roles in successful viral replication and transmission, understanding the receptor types and distribution in different tissues of domestic birds might be good initial tool to understand host factors that promote successful influenza viral infection.

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Related in: MedlinePlus

Distribution of α2,3SA-gal and α2,6SA-gal receptors along the jejunum of 4-week-old chickens (2.1.A-C), 2-week-old ducks (2.2.A-C) and 3-week-old turkeys (2.3.A-C) using plant lectins, MAA and SNA, respectively. Sections of ceca from 4-week-old chickens (2.4.A, B), 2-week-old ducks (2.5.A, B) and 3-week-old turkeys (2.6.A, B) stained with MAA and SNA respectively. Sections of colon from 4-week-old chickens (2.7.A, B), 2-week-old ducks (2.8.A, B) and 3-week-old turkeys (2.9.A, B) stained with MAA and SNA, respectively.
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Figure 2: Distribution of α2,3SA-gal and α2,6SA-gal receptors along the jejunum of 4-week-old chickens (2.1.A-C), 2-week-old ducks (2.2.A-C) and 3-week-old turkeys (2.3.A-C) using plant lectins, MAA and SNA, respectively. Sections of ceca from 4-week-old chickens (2.4.A, B), 2-week-old ducks (2.5.A, B) and 3-week-old turkeys (2.6.A, B) stained with MAA and SNA respectively. Sections of colon from 4-week-old chickens (2.7.A, B), 2-week-old ducks (2.8.A, B) and 3-week-old turkeys (2.9.A, B) stained with MAA and SNA, respectively.

Mentions: The epithelial cells of large intestine showed 30-50% staining (+++) for the presence of α2,3SA-gal receptors in 2-week-old ducks and turkeys with no positive staining for human type receptors. In 4-week-old chickens, along the epithelium of large intestine, a higher percentage of positive staining (70%, +++) was observed for avian type receptors along with the presence of human type receptors (30-50%, +++) (Fig. 2).


Species and age related differences in the type and distribution of influenza virus receptors in different tissues of chickens, ducks and turkeys.

Pillai SP, Lee CW - Virol. J. (2010)

Distribution of α2,3SA-gal and α2,6SA-gal receptors along the jejunum of 4-week-old chickens (2.1.A-C), 2-week-old ducks (2.2.A-C) and 3-week-old turkeys (2.3.A-C) using plant lectins, MAA and SNA, respectively. Sections of ceca from 4-week-old chickens (2.4.A, B), 2-week-old ducks (2.5.A, B) and 3-week-old turkeys (2.6.A, B) stained with MAA and SNA respectively. Sections of colon from 4-week-old chickens (2.7.A, B), 2-week-old ducks (2.8.A, B) and 3-week-old turkeys (2.9.A, B) stained with MAA and SNA, respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2821308&req=5

Figure 2: Distribution of α2,3SA-gal and α2,6SA-gal receptors along the jejunum of 4-week-old chickens (2.1.A-C), 2-week-old ducks (2.2.A-C) and 3-week-old turkeys (2.3.A-C) using plant lectins, MAA and SNA, respectively. Sections of ceca from 4-week-old chickens (2.4.A, B), 2-week-old ducks (2.5.A, B) and 3-week-old turkeys (2.6.A, B) stained with MAA and SNA respectively. Sections of colon from 4-week-old chickens (2.7.A, B), 2-week-old ducks (2.8.A, B) and 3-week-old turkeys (2.9.A, B) stained with MAA and SNA, respectively.
Mentions: The epithelial cells of large intestine showed 30-50% staining (+++) for the presence of α2,3SA-gal receptors in 2-week-old ducks and turkeys with no positive staining for human type receptors. In 4-week-old chickens, along the epithelium of large intestine, a higher percentage of positive staining (70%, +++) was observed for avian type receptors along with the presence of human type receptors (30-50%, +++) (Fig. 2).

Bottom Line: In contrast, a greater amount of staining for alpha2,3SA-gal (50-80%) and alpha2,6SA-gal (20-50%) receptors were observed along the epithelium of small and large intestine of chickens.Kidney and esophagus sections from the 3 bird species also expressed both avian and human type receptors.In other tissues examined, brain, breast muscles, bursa, spleen, cecal tonsils and oviduct, human type receptors were absent.

View Article: PubMed Central - HTML - PubMed

Affiliation: Food Animal Health Research Program, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, Ohio 44691, USA.

ABSTRACT
We undertook one of the most detailed studies on the distribution of alpha2,3 sialic acid (SA)-galactose (gal) (avian type) and alpha2,6SA-gal (human type) receptors on different tissues of chickens, ducks and turkeys of varying age groups. On the tracheal epithelium, all 3 bird species expressed strong positive staining (80-90%) for alpha2,3SA-gal receptors in the 3 different age groups. In addition, a lesser amount of alpha2,6SA-gal receptors (30-90%) were observed with slight differences in distribution with age and species. The epithelium of the small and large intestine of turkeys and ducks showed negligible staining for alpha2,6SA-gal receptors whereas the large intestine consistently showed 40-70% positive staining for alpha2,3SA-gal receptors. In contrast, a greater amount of staining for alpha2,3SA-gal (50-80%) and alpha2,6SA-gal (20-50%) receptors were observed along the epithelium of small and large intestine of chickens. Kidney and esophagus sections from the 3 bird species also expressed both avian and human type receptors. In other tissues examined, brain, breast muscles, bursa, spleen, cecal tonsils and oviduct, human type receptors were absent. Though different viral and receptor components may play roles in successful viral replication and transmission, understanding the receptor types and distribution in different tissues of domestic birds might be good initial tool to understand host factors that promote successful influenza viral infection.

Show MeSH
Related in: MedlinePlus