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Glycine-spacers influence functional motifs exposure and self-assembling propensity of functionalized substrates tailored for neural stem cell cultures.

Taraballi F, Natalello A, Campione M, Villa O, Doglia SM, Paleari A, Gelain F - Front Neuroeng (2010)

Bottom Line: The understanding of phenomena involved in the self-assembling of bio-inspired biomaterials acting as three-dimensional scaffolds for regenerative medicine applications is a necessary step to develop effective therapies in neural tissue engineering.Accordingly, the longer spacer of glycines, the more effective is the functional motif in both eliciting NSCs adhesion, improving their viability and increasing their differentiation.Therefore, optimized designing strategies of functionalized biomaterials may open, in the near future, new therapies in tissue engineering and regenerative medicine.

View Article: PubMed Central - PubMed

Affiliation: Center for Nanomedicine and Tissue Engineering, A.O. Ospedale Niguarda Ca' Granda Milan, Italy.

ABSTRACT
The understanding of phenomena involved in the self-assembling of bio-inspired biomaterials acting as three-dimensional scaffolds for regenerative medicine applications is a necessary step to develop effective therapies in neural tissue engineering. We investigated the self-assembled nanostructures of functionalized peptides featuring four, two or no glycine-spacers between the self-assembly sequence RADA16-I and the functional biological motif PFSSTKT. The effectiveness of their biological functionalization was assessed via in vitro experiments with neural stem cells (NSCs) and their molecular assembly was elucidated via atomic force microscopy, Raman and Fourier Transform Infrared spectroscopy. We demonstrated that glycine-spacers play a crucial role in the scaffold stability and in the exposure of the functional motifs. In particular, a glycine-spacer of four residues leads to a more stable nanostructure and to an improved exposure of the functional motif. Accordingly, the longer spacer of glycines, the more effective is the functional motif in both eliciting NSCs adhesion, improving their viability and increasing their differentiation. Therefore, optimized designing strategies of functionalized biomaterials may open, in the near future, new therapies in tissue engineering and regenerative medicine.

No MeSH data available.


Related in: MedlinePlus

ATR/FTIR absorption spectra of self-assembling peptides. (A) ATR/FTIR absorption spectra of self-assembling peptides. (B) Second derivatives of ATR/FTIR spectra in different buffer conditions. Spectra show the two intermolecular β-sheet bands around 1618 and 1696 cm−1. The highest intensity of the β-sheet components is observed for 4G-BMHP1 in assembling conditions of buffer 3 ((B), buffer 3). 0G-BMHP1 displays an up shift of the 1618 cm−1 component in buffer 4 ((B), buffer 4), indicating a loosely packed assembly.
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Figure 3: ATR/FTIR absorption spectra of self-assembling peptides. (A) ATR/FTIR absorption spectra of self-assembling peptides. (B) Second derivatives of ATR/FTIR spectra in different buffer conditions. Spectra show the two intermolecular β-sheet bands around 1618 and 1696 cm−1. The highest intensity of the β-sheet components is observed for 4G-BMHP1 in assembling conditions of buffer 3 ((B), buffer 3). 0G-BMHP1 displays an up shift of the 1618 cm−1 component in buffer 4 ((B), buffer 4), indicating a loosely packed assembly.

Mentions: Fourier Transform Infrared spectra of the peptide assemblies displayed several absorption bands in the mid-infrared region. Among them we focused our attention on the Amide I band (from 1700 to 1600 cm−1), due to the C=O stretching vibration of the peptide bond, since this particular mode is sensitive to the secondary structure and the β-sheet intermolecular interactions of the peptides (Seshadri et al., 1999; Natalello et al., 2008). Second derivatives of the absorption spectra were performed to better resolve the different spectral components overlapped in the Amide I region (Susi and Byler, 1986). ATR/FTIR measured spectra of each peptide in the different buffer conditions are reported in Figure 3A, and their second derivative spectra in Figure 3B.


Glycine-spacers influence functional motifs exposure and self-assembling propensity of functionalized substrates tailored for neural stem cell cultures.

Taraballi F, Natalello A, Campione M, Villa O, Doglia SM, Paleari A, Gelain F - Front Neuroeng (2010)

ATR/FTIR absorption spectra of self-assembling peptides. (A) ATR/FTIR absorption spectra of self-assembling peptides. (B) Second derivatives of ATR/FTIR spectra in different buffer conditions. Spectra show the two intermolecular β-sheet bands around 1618 and 1696 cm−1. The highest intensity of the β-sheet components is observed for 4G-BMHP1 in assembling conditions of buffer 3 ((B), buffer 3). 0G-BMHP1 displays an up shift of the 1618 cm−1 component in buffer 4 ((B), buffer 4), indicating a loosely packed assembly.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2821182&req=5

Figure 3: ATR/FTIR absorption spectra of self-assembling peptides. (A) ATR/FTIR absorption spectra of self-assembling peptides. (B) Second derivatives of ATR/FTIR spectra in different buffer conditions. Spectra show the two intermolecular β-sheet bands around 1618 and 1696 cm−1. The highest intensity of the β-sheet components is observed for 4G-BMHP1 in assembling conditions of buffer 3 ((B), buffer 3). 0G-BMHP1 displays an up shift of the 1618 cm−1 component in buffer 4 ((B), buffer 4), indicating a loosely packed assembly.
Mentions: Fourier Transform Infrared spectra of the peptide assemblies displayed several absorption bands in the mid-infrared region. Among them we focused our attention on the Amide I band (from 1700 to 1600 cm−1), due to the C=O stretching vibration of the peptide bond, since this particular mode is sensitive to the secondary structure and the β-sheet intermolecular interactions of the peptides (Seshadri et al., 1999; Natalello et al., 2008). Second derivatives of the absorption spectra were performed to better resolve the different spectral components overlapped in the Amide I region (Susi and Byler, 1986). ATR/FTIR measured spectra of each peptide in the different buffer conditions are reported in Figure 3A, and their second derivative spectra in Figure 3B.

Bottom Line: The understanding of phenomena involved in the self-assembling of bio-inspired biomaterials acting as three-dimensional scaffolds for regenerative medicine applications is a necessary step to develop effective therapies in neural tissue engineering.Accordingly, the longer spacer of glycines, the more effective is the functional motif in both eliciting NSCs adhesion, improving their viability and increasing their differentiation.Therefore, optimized designing strategies of functionalized biomaterials may open, in the near future, new therapies in tissue engineering and regenerative medicine.

View Article: PubMed Central - PubMed

Affiliation: Center for Nanomedicine and Tissue Engineering, A.O. Ospedale Niguarda Ca' Granda Milan, Italy.

ABSTRACT
The understanding of phenomena involved in the self-assembling of bio-inspired biomaterials acting as three-dimensional scaffolds for regenerative medicine applications is a necessary step to develop effective therapies in neural tissue engineering. We investigated the self-assembled nanostructures of functionalized peptides featuring four, two or no glycine-spacers between the self-assembly sequence RADA16-I and the functional biological motif PFSSTKT. The effectiveness of their biological functionalization was assessed via in vitro experiments with neural stem cells (NSCs) and their molecular assembly was elucidated via atomic force microscopy, Raman and Fourier Transform Infrared spectroscopy. We demonstrated that glycine-spacers play a crucial role in the scaffold stability and in the exposure of the functional motifs. In particular, a glycine-spacer of four residues leads to a more stable nanostructure and to an improved exposure of the functional motif. Accordingly, the longer spacer of glycines, the more effective is the functional motif in both eliciting NSCs adhesion, improving their viability and increasing their differentiation. Therefore, optimized designing strategies of functionalized biomaterials may open, in the near future, new therapies in tissue engineering and regenerative medicine.

No MeSH data available.


Related in: MedlinePlus