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Total hip and knee replacement surgery results in changes in leukocyte and endothelial markers.

Hughes SF, Hendricks BD, Edwards DR, Maclean KM, Bastawrous SS, Middleton JF - J Inflamm (Lond) (2010)

Bottom Line: Von Willebrand factor (vWF) and soluble intercellular adhesion molecule-1 (sICAM-1) were measured as markers of endothelial activation.Specifically, THR and TKR surgery resulted in a leukocytosis, this being demonstrated by an increase in the total leukocyte concentration following surgery.With respect to endothelial activation, increases in vWF and sICAM-1 concentrations were demonstrated following surgery.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Biological Sciences, University of Chester, UK.

ABSTRACT

Background: It is estimated that over 8 million people in the United Kingdom suffer from osteoarthritis. These patients may require orthopaedic surgical intervention to help alleviate their clinical condition. Investigations presented here was to test the hypothesis that total hip replacement (THR) and total knee replacement (TKR) orthopaedic surgery result in changes to leukocyte and endothelial markers thus increasing inflammatory reactions postoperatively.

Methods: During this 'pilot study', ten test subjects were all scheduled for THR or TKR elective surgery due to osteoarthritis. Leukocyte concentrations were measured using an automated full blood count analyser. Leukocyte CD11b (Mac-1) and CD62L cell surface expression, intracellular production of H(2)O(2 )and elastase were measured as markers of leukocyte function. Von Willebrand factor (vWF) and soluble intercellular adhesion molecule-1 (sICAM-1) were measured as markers of endothelial activation.

Results: The results obtained during this study demonstrate that THR and TKR orthopaedic surgery result in similar changes of leukocyte and endothelial markers, suggestive of increased inflammatory reactions postoperatively. Specifically, THR and TKR surgery resulted in a leukocytosis, this being demonstrated by an increase in the total leukocyte concentration following surgery. Evidence of leukocyte activation was demonstrated by a decrease in CD62L expression and an increase in CD11b expression by neutrophils and monocytes respectively. An increase in the intracellular H(2)O(2 )production by neutrophils and monocytes and in the leukocyte elastase concentrations was also evident of leukocyte activation following orthopaedic surgery. With respect to endothelial activation, increases in vWF and sICAM-1 concentrations were demonstrated following surgery.

Conclusion: In general it appeared that most of the leukocyte and endothelial markers measured during these studies peaked between days 1-3 postoperatively. It is proposed that by allowing orthopaedic surgeons access to alternative laboratory markers such as CD11b, H(2)O(2 )and elastase, CD62L, vWF and sICAM-1, an accurate assessment of the extent of inflammation due to surgery per se could be made. Ultimately, the leukocyte and endothelial markers assessed during this investigation may have a role in monitoring potential infectious complications that can occur during the postoperative period.

No MeSH data available.


Related in: MedlinePlus

Effect of THR and TKR surgery on intracellular H2O2 production of neutrophils (A) and monocytes (B). A, the points represent mean ± SD. p = 0.035, as determined by ANOVA following THR surgery. B, the points represent mean ± SD. p = 0.002, as determined by ANOVA following TKR surgery. Baseline vs day 1 postoperative following TKR (p = 0.011, as determined by pairwise comparisons) (*, p < 0.05 compared to baseline).
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Figure 4: Effect of THR and TKR surgery on intracellular H2O2 production of neutrophils (A) and monocytes (B). A, the points represent mean ± SD. p = 0.035, as determined by ANOVA following THR surgery. B, the points represent mean ± SD. p = 0.002, as determined by ANOVA following TKR surgery. Baseline vs day 1 postoperative following TKR (p = 0.011, as determined by pairwise comparisons) (*, p < 0.05 compared to baseline).

Mentions: Following THR surgery significant changes were seen in neutrophil intracellular H2O2 production (p = 0.035) (Figure 4a). Levels increased from baseline (281 ± 164) peaking at day 1 (572 ± 236) postoperatively. Intracellular H2O2 production decreased toward basal levels at day 3 (559 ± 128) and day 5 postoperative (405 ± 104).


Total hip and knee replacement surgery results in changes in leukocyte and endothelial markers.

Hughes SF, Hendricks BD, Edwards DR, Maclean KM, Bastawrous SS, Middleton JF - J Inflamm (Lond) (2010)

Effect of THR and TKR surgery on intracellular H2O2 production of neutrophils (A) and monocytes (B). A, the points represent mean ± SD. p = 0.035, as determined by ANOVA following THR surgery. B, the points represent mean ± SD. p = 0.002, as determined by ANOVA following TKR surgery. Baseline vs day 1 postoperative following TKR (p = 0.011, as determined by pairwise comparisons) (*, p < 0.05 compared to baseline).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2820000&req=5

Figure 4: Effect of THR and TKR surgery on intracellular H2O2 production of neutrophils (A) and monocytes (B). A, the points represent mean ± SD. p = 0.035, as determined by ANOVA following THR surgery. B, the points represent mean ± SD. p = 0.002, as determined by ANOVA following TKR surgery. Baseline vs day 1 postoperative following TKR (p = 0.011, as determined by pairwise comparisons) (*, p < 0.05 compared to baseline).
Mentions: Following THR surgery significant changes were seen in neutrophil intracellular H2O2 production (p = 0.035) (Figure 4a). Levels increased from baseline (281 ± 164) peaking at day 1 (572 ± 236) postoperatively. Intracellular H2O2 production decreased toward basal levels at day 3 (559 ± 128) and day 5 postoperative (405 ± 104).

Bottom Line: Von Willebrand factor (vWF) and soluble intercellular adhesion molecule-1 (sICAM-1) were measured as markers of endothelial activation.Specifically, THR and TKR surgery resulted in a leukocytosis, this being demonstrated by an increase in the total leukocyte concentration following surgery.With respect to endothelial activation, increases in vWF and sICAM-1 concentrations were demonstrated following surgery.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Biological Sciences, University of Chester, UK.

ABSTRACT

Background: It is estimated that over 8 million people in the United Kingdom suffer from osteoarthritis. These patients may require orthopaedic surgical intervention to help alleviate their clinical condition. Investigations presented here was to test the hypothesis that total hip replacement (THR) and total knee replacement (TKR) orthopaedic surgery result in changes to leukocyte and endothelial markers thus increasing inflammatory reactions postoperatively.

Methods: During this 'pilot study', ten test subjects were all scheduled for THR or TKR elective surgery due to osteoarthritis. Leukocyte concentrations were measured using an automated full blood count analyser. Leukocyte CD11b (Mac-1) and CD62L cell surface expression, intracellular production of H(2)O(2 )and elastase were measured as markers of leukocyte function. Von Willebrand factor (vWF) and soluble intercellular adhesion molecule-1 (sICAM-1) were measured as markers of endothelial activation.

Results: The results obtained during this study demonstrate that THR and TKR orthopaedic surgery result in similar changes of leukocyte and endothelial markers, suggestive of increased inflammatory reactions postoperatively. Specifically, THR and TKR surgery resulted in a leukocytosis, this being demonstrated by an increase in the total leukocyte concentration following surgery. Evidence of leukocyte activation was demonstrated by a decrease in CD62L expression and an increase in CD11b expression by neutrophils and monocytes respectively. An increase in the intracellular H(2)O(2 )production by neutrophils and monocytes and in the leukocyte elastase concentrations was also evident of leukocyte activation following orthopaedic surgery. With respect to endothelial activation, increases in vWF and sICAM-1 concentrations were demonstrated following surgery.

Conclusion: In general it appeared that most of the leukocyte and endothelial markers measured during these studies peaked between days 1-3 postoperatively. It is proposed that by allowing orthopaedic surgeons access to alternative laboratory markers such as CD11b, H(2)O(2 )and elastase, CD62L, vWF and sICAM-1, an accurate assessment of the extent of inflammation due to surgery per se could be made. Ultimately, the leukocyte and endothelial markers assessed during this investigation may have a role in monitoring potential infectious complications that can occur during the postoperative period.

No MeSH data available.


Related in: MedlinePlus