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Phylogeny and disease association of Shiga toxin-producing Escherichia coli O91.

Mellmann A, Fruth A, Friedrich AW, Wieler LH, Harmsen D, Werber D, Middendorf B, Bielaszewska M, Karch H - Emerging Infect. Dis. (2009)

Bottom Line: The diversity and relatedness of 100 Shiga toxin-producing Escherichia coli O91 isolates from different patients were examined by multilocus sequence typing.We identified 10 specific sequence types (ST) and 4 distinct clonal groups.ST442 was significantly associated with hemolytic uremic syndrome.

View Article: PubMed Central - PubMed

Affiliation: Institute of Hygiene and the National Consulting Laboratory on Hemolytic Uremic Syndrome, Münster, Germany. mellmann@uni-muenster.de

ABSTRACT
The diversity and relatedness of 100 Shiga toxin-producing Escherichia coli O91 isolates from different patients were examined by multilocus sequence typing. We identified 10 specific sequence types (ST) and 4 distinct clonal groups. ST442 was significantly associated with hemolytic uremic syndrome.

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Related in: MedlinePlus

Minimum spanning tree based on the multilocus sequence typing allelic profiles portraying the clonal distribution of the 100 Escherichia coli O91:H8/H10/H14/H21/H–/Hnt isolates (highlighted in gray) associated with different diseases in relation to the hemolytic uremic syndrome–associated enterohemorrhagic E. coli collection. Each dot represents a given sequence type, and the size of each circle is proportional to the number of strains analyzed. Connecting lines show the number of identical alleles between 2 STs (thick black line, 6 of 7 alleles identical; thick gray line, 5 alleles identical; thick dashed line, 4 alleles identical; thin dashed lines of increasing length, <3 alleles identical).
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Figure 1: Minimum spanning tree based on the multilocus sequence typing allelic profiles portraying the clonal distribution of the 100 Escherichia coli O91:H8/H10/H14/H21/H–/Hnt isolates (highlighted in gray) associated with different diseases in relation to the hemolytic uremic syndrome–associated enterohemorrhagic E. coli collection. Each dot represents a given sequence type, and the size of each circle is proportional to the number of strains analyzed. Connecting lines show the number of identical alleles between 2 STs (thick black line, 6 of 7 alleles identical; thick gray line, 5 alleles identical; thick dashed line, 4 alleles identical; thin dashed lines of increasing length, <3 alleles identical).

Mentions: The 100 STEC O91 strains resulted in 10 different STs. Of these, STs 33 and 442 were most common (63 and 20 isolates, respectively). Six additional STs (690, 1048, 1051, 1052, 1053, 1054) were single-locus variants of ST33, indicating their close relationship. The 2 remaining STs (641 and 1020) were not closely related to any other ST of the serogroup O91 strains. Detailed analysis of the 7 housekeeping genes used for MLST demonstrated the fumC gene sequence alone could differentiate 5 STs of the O91 strains. The comparison of all O91 STs with all STEC STs and serotypes associated with HUS (HUSEC collection) is displayed in the Figure.


Phylogeny and disease association of Shiga toxin-producing Escherichia coli O91.

Mellmann A, Fruth A, Friedrich AW, Wieler LH, Harmsen D, Werber D, Middendorf B, Bielaszewska M, Karch H - Emerging Infect. Dis. (2009)

Minimum spanning tree based on the multilocus sequence typing allelic profiles portraying the clonal distribution of the 100 Escherichia coli O91:H8/H10/H14/H21/H–/Hnt isolates (highlighted in gray) associated with different diseases in relation to the hemolytic uremic syndrome–associated enterohemorrhagic E. coli collection. Each dot represents a given sequence type, and the size of each circle is proportional to the number of strains analyzed. Connecting lines show the number of identical alleles between 2 STs (thick black line, 6 of 7 alleles identical; thick gray line, 5 alleles identical; thick dashed line, 4 alleles identical; thin dashed lines of increasing length, <3 alleles identical).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2819887&req=5

Figure 1: Minimum spanning tree based on the multilocus sequence typing allelic profiles portraying the clonal distribution of the 100 Escherichia coli O91:H8/H10/H14/H21/H–/Hnt isolates (highlighted in gray) associated with different diseases in relation to the hemolytic uremic syndrome–associated enterohemorrhagic E. coli collection. Each dot represents a given sequence type, and the size of each circle is proportional to the number of strains analyzed. Connecting lines show the number of identical alleles between 2 STs (thick black line, 6 of 7 alleles identical; thick gray line, 5 alleles identical; thick dashed line, 4 alleles identical; thin dashed lines of increasing length, <3 alleles identical).
Mentions: The 100 STEC O91 strains resulted in 10 different STs. Of these, STs 33 and 442 were most common (63 and 20 isolates, respectively). Six additional STs (690, 1048, 1051, 1052, 1053, 1054) were single-locus variants of ST33, indicating their close relationship. The 2 remaining STs (641 and 1020) were not closely related to any other ST of the serogroup O91 strains. Detailed analysis of the 7 housekeeping genes used for MLST demonstrated the fumC gene sequence alone could differentiate 5 STs of the O91 strains. The comparison of all O91 STs with all STEC STs and serotypes associated with HUS (HUSEC collection) is displayed in the Figure.

Bottom Line: The diversity and relatedness of 100 Shiga toxin-producing Escherichia coli O91 isolates from different patients were examined by multilocus sequence typing.We identified 10 specific sequence types (ST) and 4 distinct clonal groups.ST442 was significantly associated with hemolytic uremic syndrome.

View Article: PubMed Central - PubMed

Affiliation: Institute of Hygiene and the National Consulting Laboratory on Hemolytic Uremic Syndrome, Münster, Germany. mellmann@uni-muenster.de

ABSTRACT
The diversity and relatedness of 100 Shiga toxin-producing Escherichia coli O91 isolates from different patients were examined by multilocus sequence typing. We identified 10 specific sequence types (ST) and 4 distinct clonal groups. ST442 was significantly associated with hemolytic uremic syndrome.

Show MeSH
Related in: MedlinePlus