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Avian bornaviruses in psittacine birds from Europe and Australia with proventricular dilatation disease.

Weissenböck H, Bakonyi T, Sekulin K, Ehrensperger F, Doneley RJ, Dürrwald R, Hoop R, Erdélyi K, Gál J, Kolodziejek J, Nowotny N - Emerging Infect. Dis. (2009)

Bottom Line: Immunohistochemical demonstration of viral antigen within the brain and vegetative nerve system of the gastrointestinal tract provides strong evidence for a causative role of ABVs in this condition.Partial sequences of nucleoprotein (p40) and matrix protein (gp18) genes showed that virus in most of our cases belonged to the ABV-2 and ABV-4 groups among the 5 genogroups described so far.Viral sequences of 2 birds did not match any of the described sequences and clustered together in a new branch termed ABV-6.

View Article: PubMed Central - PubMed

Affiliation: University of Veterinary Medicine, Vienna, Austria.

ABSTRACT
To determine whether avian bornaviruses (ABVs) were a factor in proventricular dilatation disease (PDD), we used immunohistochemistry, reverse transcription-PCR, and nucleotide sequence analysis to examine paraffin wax-embedded or frozen tissue samples of 31 psittacine birds with this disease. PDD is a fatal disease of psittacine birds associated with nonsuppurative encephalitis and ganglioneuritis of the upper intestinal tract. Tissue samples had been collected from 1999 through 2008 in Austria, Switzerland, Hungary, and Australia. Immunohistochemical demonstration of viral antigen within the brain and vegetative nerve system of the gastrointestinal tract provides strong evidence for a causative role of ABVs in this condition. Partial sequences of nucleoprotein (p40) and matrix protein (gp18) genes showed that virus in most of our cases belonged to the ABV-2 and ABV-4 groups among the 5 genogroups described so far. Viral sequences of 2 birds did not match any of the described sequences and clustered together in a new branch termed ABV-6.

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Related in: MedlinePlus

Phylogram illustrating the genetic relationship among avian bornavirus (ABV) genotypes, based on a partial matrix protein gene region. Three representatives of BDV were used as outgroups. Scale bar indicates genetic distance; the bootstrap support values are shown for the main nodes. ABVs are identified by GenBank accession number/country of origin/year of collection. Further details are shown in the Appendix Table. Nucleotide sequences determined in this study are highlighted in different colors according to their country of origin. The main ABV genogroups are indicated.
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Figure 4: Phylogram illustrating the genetic relationship among avian bornavirus (ABV) genotypes, based on a partial matrix protein gene region. Three representatives of BDV were used as outgroups. Scale bar indicates genetic distance; the bootstrap support values are shown for the main nodes. ABVs are identified by GenBank accession number/country of origin/year of collection. Further details are shown in the Appendix Table. Nucleotide sequences determined in this study are highlighted in different colors according to their country of origin. The main ABV genogroups are indicated.

Mentions: Phylogenetic trees (Figures 3, 4), based on the N and M protein gene regions, showed similar structures. The sequences did not exhibit any clustering according to either the collection year, country of origin, or host species. ABV sequences from different countries in Europe and from Australia were similar, or even homologous to each other. Moreover, the newly determined sequences proved to be frequently similar or homologous to GenBank sequences derived from samples collected in the United States and in Israel. The newly identified sequences clustered within the same main branches in the N and M gene trees. Two similar M protein gene sequences (281–01 and H03–2080), however, exhibited low identity rates to all other sequences and formed a distinct branch in the phylogenetic tree, which was clearly separated from all other groups of ABVs and mammalian bornaviruses. Consequently, we suggest that this unique cluster be accepted as a novel ABV genotype, designated ABV-6. The bootstrap analysis supported the main clustering of the consensus trees.


Avian bornaviruses in psittacine birds from Europe and Australia with proventricular dilatation disease.

Weissenböck H, Bakonyi T, Sekulin K, Ehrensperger F, Doneley RJ, Dürrwald R, Hoop R, Erdélyi K, Gál J, Kolodziejek J, Nowotny N - Emerging Infect. Dis. (2009)

Phylogram illustrating the genetic relationship among avian bornavirus (ABV) genotypes, based on a partial matrix protein gene region. Three representatives of BDV were used as outgroups. Scale bar indicates genetic distance; the bootstrap support values are shown for the main nodes. ABVs are identified by GenBank accession number/country of origin/year of collection. Further details are shown in the Appendix Table. Nucleotide sequences determined in this study are highlighted in different colors according to their country of origin. The main ABV genogroups are indicated.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2819881&req=5

Figure 4: Phylogram illustrating the genetic relationship among avian bornavirus (ABV) genotypes, based on a partial matrix protein gene region. Three representatives of BDV were used as outgroups. Scale bar indicates genetic distance; the bootstrap support values are shown for the main nodes. ABVs are identified by GenBank accession number/country of origin/year of collection. Further details are shown in the Appendix Table. Nucleotide sequences determined in this study are highlighted in different colors according to their country of origin. The main ABV genogroups are indicated.
Mentions: Phylogenetic trees (Figures 3, 4), based on the N and M protein gene regions, showed similar structures. The sequences did not exhibit any clustering according to either the collection year, country of origin, or host species. ABV sequences from different countries in Europe and from Australia were similar, or even homologous to each other. Moreover, the newly determined sequences proved to be frequently similar or homologous to GenBank sequences derived from samples collected in the United States and in Israel. The newly identified sequences clustered within the same main branches in the N and M gene trees. Two similar M protein gene sequences (281–01 and H03–2080), however, exhibited low identity rates to all other sequences and formed a distinct branch in the phylogenetic tree, which was clearly separated from all other groups of ABVs and mammalian bornaviruses. Consequently, we suggest that this unique cluster be accepted as a novel ABV genotype, designated ABV-6. The bootstrap analysis supported the main clustering of the consensus trees.

Bottom Line: Immunohistochemical demonstration of viral antigen within the brain and vegetative nerve system of the gastrointestinal tract provides strong evidence for a causative role of ABVs in this condition.Partial sequences of nucleoprotein (p40) and matrix protein (gp18) genes showed that virus in most of our cases belonged to the ABV-2 and ABV-4 groups among the 5 genogroups described so far.Viral sequences of 2 birds did not match any of the described sequences and clustered together in a new branch termed ABV-6.

View Article: PubMed Central - PubMed

Affiliation: University of Veterinary Medicine, Vienna, Austria.

ABSTRACT
To determine whether avian bornaviruses (ABVs) were a factor in proventricular dilatation disease (PDD), we used immunohistochemistry, reverse transcription-PCR, and nucleotide sequence analysis to examine paraffin wax-embedded or frozen tissue samples of 31 psittacine birds with this disease. PDD is a fatal disease of psittacine birds associated with nonsuppurative encephalitis and ganglioneuritis of the upper intestinal tract. Tissue samples had been collected from 1999 through 2008 in Austria, Switzerland, Hungary, and Australia. Immunohistochemical demonstration of viral antigen within the brain and vegetative nerve system of the gastrointestinal tract provides strong evidence for a causative role of ABVs in this condition. Partial sequences of nucleoprotein (p40) and matrix protein (gp18) genes showed that virus in most of our cases belonged to the ABV-2 and ABV-4 groups among the 5 genogroups described so far. Viral sequences of 2 birds did not match any of the described sequences and clustered together in a new branch termed ABV-6.

Show MeSH
Related in: MedlinePlus