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Human Plasmodium knowlesi infection detected by rapid diagnostic tests for malaria.

van Hellemond JJ, Rutten M, Koelewijn R, Zeeman AM, Verweij JJ, Wismans PJ, Kocken CH, van Genderen PJ - Emerging Infect. Dis. (2009)

Bottom Line: We describe a PCR-confirmed case of Plasmodium knowlesi infection with a high parasitemia level and clinical signs of severe malaria in a migrant worker from Malaysian Borneo in the Netherlands.Investigations showed that commercially available rapid antigen tests for detection of human Plasmodium infections can detect P. knowlesi infections in humans.

View Article: PubMed Central - PubMed

Affiliation: Erasmus University Medical Center, Rotterdam, the Netherlands.

ABSTRACT
We describe a PCR-confirmed case of Plasmodium knowlesi infection with a high parasitemia level and clinical signs of severe malaria in a migrant worker from Malaysian Borneo in the Netherlands. Investigations showed that commercially available rapid antigen tests for detection of human Plasmodium infections can detect P. knowlesi infections in humans.

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Related in: MedlinePlus

Phylogenetic tree constructed according to the neighbor-joining method based on A-type small subunit RNA sequences of several Plasmodium species (GenBank accession numbers are indicated). The sequence of the clinical isolate PkHHR-BPRC1 (in boldface) (GenBank accession no. FJ804768) clusters with all other P. knowlesi strains (indicated by Pk isolate numbers). Pfrag, P. fragile; Pinui, P. inui; Pcyn, P. cynomolgi; Pfalc, P. falciparum; Pmal, P. malariae. Scale bar indicates nucleotide substitutions per site.
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Figure 2: Phylogenetic tree constructed according to the neighbor-joining method based on A-type small subunit RNA sequences of several Plasmodium species (GenBank accession numbers are indicated). The sequence of the clinical isolate PkHHR-BPRC1 (in boldface) (GenBank accession no. FJ804768) clusters with all other P. knowlesi strains (indicated by Pk isolate numbers). Pfrag, P. fragile; Pinui, P. inui; Pcyn, P. cynomolgi; Pfalc, P. falciparum; Pmal, P. malariae. Scale bar indicates nucleotide substitutions per site.

Mentions: To confirm the PCR result, we sequenced the cloned amplification product generated with primers rPLU1 and rPLU5. Sequences were compared with known Plasmodium A-type SSU rRNA sequences by using the neighbor-joining method (Figure 2). The sequence of the clinical isolate PkHHR-BPRC1 (GenBank accession no. FJ804768) clustered strongly with P. knowlesi A-type SSU RNA sequences, confirming that the patient was infected with the P. knowlesi parasite.


Human Plasmodium knowlesi infection detected by rapid diagnostic tests for malaria.

van Hellemond JJ, Rutten M, Koelewijn R, Zeeman AM, Verweij JJ, Wismans PJ, Kocken CH, van Genderen PJ - Emerging Infect. Dis. (2009)

Phylogenetic tree constructed according to the neighbor-joining method based on A-type small subunit RNA sequences of several Plasmodium species (GenBank accession numbers are indicated). The sequence of the clinical isolate PkHHR-BPRC1 (in boldface) (GenBank accession no. FJ804768) clusters with all other P. knowlesi strains (indicated by Pk isolate numbers). Pfrag, P. fragile; Pinui, P. inui; Pcyn, P. cynomolgi; Pfalc, P. falciparum; Pmal, P. malariae. Scale bar indicates nucleotide substitutions per site.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2819855&req=5

Figure 2: Phylogenetic tree constructed according to the neighbor-joining method based on A-type small subunit RNA sequences of several Plasmodium species (GenBank accession numbers are indicated). The sequence of the clinical isolate PkHHR-BPRC1 (in boldface) (GenBank accession no. FJ804768) clusters with all other P. knowlesi strains (indicated by Pk isolate numbers). Pfrag, P. fragile; Pinui, P. inui; Pcyn, P. cynomolgi; Pfalc, P. falciparum; Pmal, P. malariae. Scale bar indicates nucleotide substitutions per site.
Mentions: To confirm the PCR result, we sequenced the cloned amplification product generated with primers rPLU1 and rPLU5. Sequences were compared with known Plasmodium A-type SSU rRNA sequences by using the neighbor-joining method (Figure 2). The sequence of the clinical isolate PkHHR-BPRC1 (GenBank accession no. FJ804768) clustered strongly with P. knowlesi A-type SSU RNA sequences, confirming that the patient was infected with the P. knowlesi parasite.

Bottom Line: We describe a PCR-confirmed case of Plasmodium knowlesi infection with a high parasitemia level and clinical signs of severe malaria in a migrant worker from Malaysian Borneo in the Netherlands.Investigations showed that commercially available rapid antigen tests for detection of human Plasmodium infections can detect P. knowlesi infections in humans.

View Article: PubMed Central - PubMed

Affiliation: Erasmus University Medical Center, Rotterdam, the Netherlands.

ABSTRACT
We describe a PCR-confirmed case of Plasmodium knowlesi infection with a high parasitemia level and clinical signs of severe malaria in a migrant worker from Malaysian Borneo in the Netherlands. Investigations showed that commercially available rapid antigen tests for detection of human Plasmodium infections can detect P. knowlesi infections in humans.

Show MeSH
Related in: MedlinePlus