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Human Plasmodium knowlesi infection detected by rapid diagnostic tests for malaria.

van Hellemond JJ, Rutten M, Koelewijn R, Zeeman AM, Verweij JJ, Wismans PJ, Kocken CH, van Genderen PJ - Emerging Infect. Dis. (2009)

Bottom Line: We describe a PCR-confirmed case of Plasmodium knowlesi infection with a high parasitemia level and clinical signs of severe malaria in a migrant worker from Malaysian Borneo in the Netherlands.Investigations showed that commercially available rapid antigen tests for detection of human Plasmodium infections can detect P. knowlesi infections in humans.

View Article: PubMed Central - PubMed

Affiliation: Erasmus University Medical Center, Rotterdam, the Netherlands.

ABSTRACT
We describe a PCR-confirmed case of Plasmodium knowlesi infection with a high parasitemia level and clinical signs of severe malaria in a migrant worker from Malaysian Borneo in the Netherlands. Investigations showed that commercially available rapid antigen tests for detection of human Plasmodium infections can detect P. knowlesi infections in humans.

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Related in: MedlinePlus

Morphology of Plasmodium knowlesi in a Giemsa-stained thin blood smear. Infected erythrocytes were not enlarged, lacked Schuffner stippling, and contained much pigment. Shown are examples of trophozoites (A–F), a schizont (G), and a gametocyte (H). Scale bars = 5 μm.
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Figure 1: Morphology of Plasmodium knowlesi in a Giemsa-stained thin blood smear. Infected erythrocytes were not enlarged, lacked Schuffner stippling, and contained much pigment. Shown are examples of trophozoites (A–F), a schizont (G), and a gametocyte (H). Scale bars = 5 μm.

Mentions: In a rapid diagnostic test for malaria (BinaxNOW Malaria Test; Binax, Inc., Scarborough, ME, USA), his blood sample was negative for P. falciparum histidine-rich protein 2 but showed a positive reaction with pan-malarial aldolase antigen, which suggested a non–P. falciparum infection. Results of quantitative buffy coat analysis were positive for malaria trophozoites, schizonts, and gametocytes. A thin blood film showed parasite density of 2% infected erythrocytes (84,000 trophozoites/μL), schizonts, and gametocytes with an inconclusive morphologic appearance (Figure 1). A P. knowlesi infection was suspected because of his recent stay in Kapit, Malaysian Borneo. The patient was treated orally with chloroquine, 10 mg/kg, followed by 5 mg/kg after 6, 24, and 48 hours, which resulted in a rapid relief of symptoms and fever. Results of quantitative buffy coat analysis, pan-malarial aldolase antigen reactivity, and thick and thin blood smears were negative within 40 hours after administration of chloroquine.


Human Plasmodium knowlesi infection detected by rapid diagnostic tests for malaria.

van Hellemond JJ, Rutten M, Koelewijn R, Zeeman AM, Verweij JJ, Wismans PJ, Kocken CH, van Genderen PJ - Emerging Infect. Dis. (2009)

Morphology of Plasmodium knowlesi in a Giemsa-stained thin blood smear. Infected erythrocytes were not enlarged, lacked Schuffner stippling, and contained much pigment. Shown are examples of trophozoites (A–F), a schizont (G), and a gametocyte (H). Scale bars = 5 μm.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2819855&req=5

Figure 1: Morphology of Plasmodium knowlesi in a Giemsa-stained thin blood smear. Infected erythrocytes were not enlarged, lacked Schuffner stippling, and contained much pigment. Shown are examples of trophozoites (A–F), a schizont (G), and a gametocyte (H). Scale bars = 5 μm.
Mentions: In a rapid diagnostic test for malaria (BinaxNOW Malaria Test; Binax, Inc., Scarborough, ME, USA), his blood sample was negative for P. falciparum histidine-rich protein 2 but showed a positive reaction with pan-malarial aldolase antigen, which suggested a non–P. falciparum infection. Results of quantitative buffy coat analysis were positive for malaria trophozoites, schizonts, and gametocytes. A thin blood film showed parasite density of 2% infected erythrocytes (84,000 trophozoites/μL), schizonts, and gametocytes with an inconclusive morphologic appearance (Figure 1). A P. knowlesi infection was suspected because of his recent stay in Kapit, Malaysian Borneo. The patient was treated orally with chloroquine, 10 mg/kg, followed by 5 mg/kg after 6, 24, and 48 hours, which resulted in a rapid relief of symptoms and fever. Results of quantitative buffy coat analysis, pan-malarial aldolase antigen reactivity, and thick and thin blood smears were negative within 40 hours after administration of chloroquine.

Bottom Line: We describe a PCR-confirmed case of Plasmodium knowlesi infection with a high parasitemia level and clinical signs of severe malaria in a migrant worker from Malaysian Borneo in the Netherlands.Investigations showed that commercially available rapid antigen tests for detection of human Plasmodium infections can detect P. knowlesi infections in humans.

View Article: PubMed Central - PubMed

Affiliation: Erasmus University Medical Center, Rotterdam, the Netherlands.

ABSTRACT
We describe a PCR-confirmed case of Plasmodium knowlesi infection with a high parasitemia level and clinical signs of severe malaria in a migrant worker from Malaysian Borneo in the Netherlands. Investigations showed that commercially available rapid antigen tests for detection of human Plasmodium infections can detect P. knowlesi infections in humans.

Show MeSH
Related in: MedlinePlus