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Distant relatives of severe acute respiratory syndrome coronavirus and close relatives of human coronavirus 229E in bats, Ghana.

Pfefferle S, Oppong S, Drexler JF, Gloza-Rausch F, Ipsen A, Seebens A, Müller MA, Annan A, Vallo P, Adu-Sarkodie Y, Kruppa TF, Drosten C - Emerging Infect. Dis. (2009)

Bottom Line: Leaf-nosed bats pertaining to Hipposideros ruber by morphology had group 1 and group 2 CoVs.The most recent common ancestor of hCoV-229E and GhanaBt-CoVGrp1 existed in approximately 1686-1800 ad.The GhanaBt-CoVGrp2 shared an old ancestor (approximately 2,400 years) with the severe acute respiratory syndrome-like group of CoV.

View Article: PubMed Central - PubMed

Affiliation: Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.

ABSTRACT
We tested 12 bat species in Ghana for coronavirus (CoV) RNA. The virus prevalence in insectivorous bats (n = 123) was 9.76%. CoV was not detected in 212 fecal samples from Eidolon helvum fruit bats. Leaf-nosed bats pertaining to Hipposideros ruber by morphology had group 1 and group 2 CoVs. Virus concentrations were < or =45,000 copies/100 mg of bat feces. The diversified group 1 CoV shared a common ancestor with the human common cold virus hCoV-229E but not with hCoV-NL63, disputing hypotheses of common human descent. The most recent common ancestor of hCoV-229E and GhanaBt-CoVGrp1 existed in approximately 1686-1800 ad. The GhanaBt-CoVGrp2 shared an old ancestor (approximately 2,400 years) with the severe acute respiratory syndrome-like group of CoV.

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Location of Kwamang caves near the village of Kwamang, (6°58′N, 1°16′W), 50 km northeast of Kumasi, Ashanti region, Ghana. Booyem caves A (7°43′24.9′′N, 1°59′16.5′W) and B (7°43′25.7′′N, 1°59′33.5′′W) are located near remote small settlements in the vicinity of Booyem, Brong-Ahafo region. Lake Bosumtwi is located 30 km southeast of Kumasi (6°32′22.3′′N, 1°24′41.5′′W). The botanical gardens of Kwame Nkrumah National University of Science and Technology are located on campus in the city of Kumasi (6°41′6.4′′N, 1°33′42.8′′W). Kumasi Zoo is located in the center of the city (6°42′2.0′′N, 1°37′29.9′′W).
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Figure 1: Location of Kwamang caves near the village of Kwamang, (6°58′N, 1°16′W), 50 km northeast of Kumasi, Ashanti region, Ghana. Booyem caves A (7°43′24.9′′N, 1°59′16.5′W) and B (7°43′25.7′′N, 1°59′33.5′′W) are located near remote small settlements in the vicinity of Booyem, Brong-Ahafo region. Lake Bosumtwi is located 30 km southeast of Kumasi (6°32′22.3′′N, 1°24′41.5′′W). The botanical gardens of Kwame Nkrumah National University of Science and Technology are located on campus in the city of Kumasi (6°41′6.4′′N, 1°33′42.8′′W). Kumasi Zoo is located in the center of the city (6°42′2.0′′N, 1°37′29.9′′W).

Mentions: In the locations identified in Figure 1, mist netting and sampling were conducted as described (11). In Kumasi Zoo, fecal samples were collected with plastic foil under trees occupied by Eidolon helvum bats (estimated colony size 300,000). For all capturing and sampling, permission was obtained from the Wildlife Division of the Ministry of Lands, Forestry, and Mines in Ghana. Research samples were exported under a state agreement between the Republic of Ghana and the Federal Republic of Germany, represented by the City of Hamburg. Additional export permission was obtained from the Veterinary Services of the Ghana Ministry of Food and Agriculture.


Distant relatives of severe acute respiratory syndrome coronavirus and close relatives of human coronavirus 229E in bats, Ghana.

Pfefferle S, Oppong S, Drexler JF, Gloza-Rausch F, Ipsen A, Seebens A, Müller MA, Annan A, Vallo P, Adu-Sarkodie Y, Kruppa TF, Drosten C - Emerging Infect. Dis. (2009)

Location of Kwamang caves near the village of Kwamang, (6°58′N, 1°16′W), 50 km northeast of Kumasi, Ashanti region, Ghana. Booyem caves A (7°43′24.9′′N, 1°59′16.5′W) and B (7°43′25.7′′N, 1°59′33.5′′W) are located near remote small settlements in the vicinity of Booyem, Brong-Ahafo region. Lake Bosumtwi is located 30 km southeast of Kumasi (6°32′22.3′′N, 1°24′41.5′′W). The botanical gardens of Kwame Nkrumah National University of Science and Technology are located on campus in the city of Kumasi (6°41′6.4′′N, 1°33′42.8′′W). Kumasi Zoo is located in the center of the city (6°42′2.0′′N, 1°37′29.9′′W).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2819850&req=5

Figure 1: Location of Kwamang caves near the village of Kwamang, (6°58′N, 1°16′W), 50 km northeast of Kumasi, Ashanti region, Ghana. Booyem caves A (7°43′24.9′′N, 1°59′16.5′W) and B (7°43′25.7′′N, 1°59′33.5′′W) are located near remote small settlements in the vicinity of Booyem, Brong-Ahafo region. Lake Bosumtwi is located 30 km southeast of Kumasi (6°32′22.3′′N, 1°24′41.5′′W). The botanical gardens of Kwame Nkrumah National University of Science and Technology are located on campus in the city of Kumasi (6°41′6.4′′N, 1°33′42.8′′W). Kumasi Zoo is located in the center of the city (6°42′2.0′′N, 1°37′29.9′′W).
Mentions: In the locations identified in Figure 1, mist netting and sampling were conducted as described (11). In Kumasi Zoo, fecal samples were collected with plastic foil under trees occupied by Eidolon helvum bats (estimated colony size 300,000). For all capturing and sampling, permission was obtained from the Wildlife Division of the Ministry of Lands, Forestry, and Mines in Ghana. Research samples were exported under a state agreement between the Republic of Ghana and the Federal Republic of Germany, represented by the City of Hamburg. Additional export permission was obtained from the Veterinary Services of the Ghana Ministry of Food and Agriculture.

Bottom Line: Leaf-nosed bats pertaining to Hipposideros ruber by morphology had group 1 and group 2 CoVs.The most recent common ancestor of hCoV-229E and GhanaBt-CoVGrp1 existed in approximately 1686-1800 ad.The GhanaBt-CoVGrp2 shared an old ancestor (approximately 2,400 years) with the severe acute respiratory syndrome-like group of CoV.

View Article: PubMed Central - PubMed

Affiliation: Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.

ABSTRACT
We tested 12 bat species in Ghana for coronavirus (CoV) RNA. The virus prevalence in insectivorous bats (n = 123) was 9.76%. CoV was not detected in 212 fecal samples from Eidolon helvum fruit bats. Leaf-nosed bats pertaining to Hipposideros ruber by morphology had group 1 and group 2 CoVs. Virus concentrations were < or =45,000 copies/100 mg of bat feces. The diversified group 1 CoV shared a common ancestor with the human common cold virus hCoV-229E but not with hCoV-NL63, disputing hypotheses of common human descent. The most recent common ancestor of hCoV-229E and GhanaBt-CoVGrp1 existed in approximately 1686-1800 ad. The GhanaBt-CoVGrp2 shared an old ancestor (approximately 2,400 years) with the severe acute respiratory syndrome-like group of CoV.

Show MeSH
Related in: MedlinePlus