Limits...
Evaluation of ultraviolet light toxicity on cultured retinal pigment epithelial and retinal ganglion cells.

Balaiya S, Murthy RK, Brar VS, Chalam KV - Clin Ophthalmol (2010)

Bottom Line: They were subsequently examined for changes in cell morphology, cell viability (neutral red uptake assay), generation of reactive oxygen species (ROS), expression of bax, bcl-2 and cytochome C by reverse transcriptase polymerase chain reaction and western blot, respectively.Dose-dependent reduction in cell viability to UVB light was demonstrated with parallel increase in ROS.The concomitant generation of ROS and expression of apoptotic markers suggests the role of oxidative stress in UVB-mediated apoptosis in an in vitro model of retinal ganglion and pigment epithelial cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, University of Florida College of Medicine, Jacksonville, FL, USA.

ABSTRACT

Purpose: Our study is aimed at evaluating the role of UVB light in inducing cytotoxicity in an in vitro model.

Methods: RGC-5 and ARPE-19 cells were exposed to different time periods of UVB light: 0, 15, 30, and 45 min. They were subsequently examined for changes in cell morphology, cell viability (neutral red uptake assay), generation of reactive oxygen species (ROS), expression of bax, bcl-2 and cytochome C by reverse transcriptase polymerase chain reaction and western blot, respectively.

Results: Dose-dependent reduction in cell viability to UVB light was demonstrated with parallel increase in ROS. Increased duration of exposure (>15 minutes), was associated with increased expression of bax and cytochrome C, and absence of bcl-2 expression.

Conclusion: UVB light exposure results in cell cytotoxicity. The concomitant generation of ROS and expression of apoptotic markers suggests the role of oxidative stress in UVB-mediated apoptosis in an in vitro model of retinal ganglion and pigment epithelial cells.

No MeSH data available.


Related in: MedlinePlus

Phase contrast micrographs shows the presence of morphological changes such as bleb formation, vacuolation, and membrane rupture observed in ARPE-19 cells after various time points of UVB exposure.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC2819767&req=5

f2-opth-4-033: Phase contrast micrographs shows the presence of morphological changes such as bleb formation, vacuolation, and membrane rupture observed in ARPE-19 cells after various time points of UVB exposure.

Mentions: In ARPE-19 cells, 22% developed blebs after 15 min of UV exposure. Other apoptotic features like large vacuoles were present in 12% of the cells after 30 min of UV exposure. With 45 min of exposure, 90% of the cells showed increase in size with irregular cell borders. Membrane rupture was observed in 33% after 30 min exposure which increased to 47% after 45 min of UV exposure (Figure 2).


Evaluation of ultraviolet light toxicity on cultured retinal pigment epithelial and retinal ganglion cells.

Balaiya S, Murthy RK, Brar VS, Chalam KV - Clin Ophthalmol (2010)

Phase contrast micrographs shows the presence of morphological changes such as bleb formation, vacuolation, and membrane rupture observed in ARPE-19 cells after various time points of UVB exposure.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2819767&req=5

f2-opth-4-033: Phase contrast micrographs shows the presence of morphological changes such as bleb formation, vacuolation, and membrane rupture observed in ARPE-19 cells after various time points of UVB exposure.
Mentions: In ARPE-19 cells, 22% developed blebs after 15 min of UV exposure. Other apoptotic features like large vacuoles were present in 12% of the cells after 30 min of UV exposure. With 45 min of exposure, 90% of the cells showed increase in size with irregular cell borders. Membrane rupture was observed in 33% after 30 min exposure which increased to 47% after 45 min of UV exposure (Figure 2).

Bottom Line: They were subsequently examined for changes in cell morphology, cell viability (neutral red uptake assay), generation of reactive oxygen species (ROS), expression of bax, bcl-2 and cytochome C by reverse transcriptase polymerase chain reaction and western blot, respectively.Dose-dependent reduction in cell viability to UVB light was demonstrated with parallel increase in ROS.The concomitant generation of ROS and expression of apoptotic markers suggests the role of oxidative stress in UVB-mediated apoptosis in an in vitro model of retinal ganglion and pigment epithelial cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, University of Florida College of Medicine, Jacksonville, FL, USA.

ABSTRACT

Purpose: Our study is aimed at evaluating the role of UVB light in inducing cytotoxicity in an in vitro model.

Methods: RGC-5 and ARPE-19 cells were exposed to different time periods of UVB light: 0, 15, 30, and 45 min. They were subsequently examined for changes in cell morphology, cell viability (neutral red uptake assay), generation of reactive oxygen species (ROS), expression of bax, bcl-2 and cytochome C by reverse transcriptase polymerase chain reaction and western blot, respectively.

Results: Dose-dependent reduction in cell viability to UVB light was demonstrated with parallel increase in ROS. Increased duration of exposure (>15 minutes), was associated with increased expression of bax and cytochrome C, and absence of bcl-2 expression.

Conclusion: UVB light exposure results in cell cytotoxicity. The concomitant generation of ROS and expression of apoptotic markers suggests the role of oxidative stress in UVB-mediated apoptosis in an in vitro model of retinal ganglion and pigment epithelial cells.

No MeSH data available.


Related in: MedlinePlus