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Trimetazidine for prevention of induced ischemia and reperfusion of guinea pig retina.

Demir T, Turgut B, Ozercan I, Gul FC, Ilhan N, Celiker U - Clin Ophthalmol (2010)

Bottom Line: The mean free MDA level increased in the placebo group (P = 0.006) but not in the drug group (P > 0.05).We observed polymorphonucleated leukocyte infiltration, retinal edema and hydropic degeneration in the retina of the placebo group.However, significant histopathologic change was not observed in specimens of the drug group.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Firat University School of Medicine, Elazig, Turkey.

ABSTRACT

Objective: Trimetazidine (TMZ) has been used to protect against ischemia/reperfusion (I/R) injury of many tissues. We aimed to evaluate the effect of TMZ during retinal I/R in a guinea pig model.

Study design/patients and methods: An experimental study in retinal I/R. Three groups of five guinea pigs were studied to include a control, placebo, and drug test groups. Prior to the application of 90 minutes of high intraocular pressure (IOP) to induce retinal ischemia followed by 24 hours of reperfusion, we applied intraperitoneal saline to the placebo group and 3 mg/kg of TMZ for the drug test group and repeated the injections at intervals of six hours for four cycles. Both eyes of the animals were enucleated at the end of the reperfusion period. Biochemical assay and histopathologic evaluation was performed on one randomly selected eye of each animal. The level of retinal-free malondialdehyde (MDA) and retinal layer thicknesses were determined and comparisons were then made with the control group.

Results: The mean free MDA level increased in the placebo group (P = 0.006) but not in the drug group (P > 0.05). We observed polymorphonucleated leukocyte infiltration, retinal edema and hydropic degeneration in the retina of the placebo group. However, significant histopathologic change was not observed in specimens of the drug group.

Conclusions: This study suggests TMZ has a beneficial effect on retinal lipid peroxidation and histopathologic changes due to I/R injury.

No MeSH data available.


Related in: MedlinePlus

Placebo: Microphotograph of degeneration of retinal ganglion cells (broken arrow), edema of inner plexiform layer (long arrow), pyknotic cells in inner nuclear layer (short arrow) and polymorphonuclear leukocytes (thick arrow).Notes: Hematoxylin and eosin 400 × magnification.
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f2-opth-4-021: Placebo: Microphotograph of degeneration of retinal ganglion cells (broken arrow), edema of inner plexiform layer (long arrow), pyknotic cells in inner nuclear layer (short arrow) and polymorphonuclear leukocytes (thick arrow).Notes: Hematoxylin and eosin 400 × magnification.

Mentions: The thickness of the retinal layers of the groups is presented in Table 1 and Figure 1. The histopathologic specimens of the control and the placebo group were compared. Observed differences were mainly in the inner retina of the placebo group. Prominent edema in the inner plexiform and ganglion cell layers, polymorphonuclear leucocyte (PMNL) infiltration and vacuolated spaces, hydropic degeneration, pyknosis particularly in the inner nuclear and ganglion cell layers were the main changes that were observed in the placebo group when compared to the control group (Figure 2). Additionally prominent edema was also observed in the outer plexiform and outer nuclear layers of the placebo group. Only mild edema was observed in the inner retina of the drug group (Figure 3).


Trimetazidine for prevention of induced ischemia and reperfusion of guinea pig retina.

Demir T, Turgut B, Ozercan I, Gul FC, Ilhan N, Celiker U - Clin Ophthalmol (2010)

Placebo: Microphotograph of degeneration of retinal ganglion cells (broken arrow), edema of inner plexiform layer (long arrow), pyknotic cells in inner nuclear layer (short arrow) and polymorphonuclear leukocytes (thick arrow).Notes: Hematoxylin and eosin 400 × magnification.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2819765&req=5

f2-opth-4-021: Placebo: Microphotograph of degeneration of retinal ganglion cells (broken arrow), edema of inner plexiform layer (long arrow), pyknotic cells in inner nuclear layer (short arrow) and polymorphonuclear leukocytes (thick arrow).Notes: Hematoxylin and eosin 400 × magnification.
Mentions: The thickness of the retinal layers of the groups is presented in Table 1 and Figure 1. The histopathologic specimens of the control and the placebo group were compared. Observed differences were mainly in the inner retina of the placebo group. Prominent edema in the inner plexiform and ganglion cell layers, polymorphonuclear leucocyte (PMNL) infiltration and vacuolated spaces, hydropic degeneration, pyknosis particularly in the inner nuclear and ganglion cell layers were the main changes that were observed in the placebo group when compared to the control group (Figure 2). Additionally prominent edema was also observed in the outer plexiform and outer nuclear layers of the placebo group. Only mild edema was observed in the inner retina of the drug group (Figure 3).

Bottom Line: The mean free MDA level increased in the placebo group (P = 0.006) but not in the drug group (P > 0.05).We observed polymorphonucleated leukocyte infiltration, retinal edema and hydropic degeneration in the retina of the placebo group.However, significant histopathologic change was not observed in specimens of the drug group.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Firat University School of Medicine, Elazig, Turkey.

ABSTRACT

Objective: Trimetazidine (TMZ) has been used to protect against ischemia/reperfusion (I/R) injury of many tissues. We aimed to evaluate the effect of TMZ during retinal I/R in a guinea pig model.

Study design/patients and methods: An experimental study in retinal I/R. Three groups of five guinea pigs were studied to include a control, placebo, and drug test groups. Prior to the application of 90 minutes of high intraocular pressure (IOP) to induce retinal ischemia followed by 24 hours of reperfusion, we applied intraperitoneal saline to the placebo group and 3 mg/kg of TMZ for the drug test group and repeated the injections at intervals of six hours for four cycles. Both eyes of the animals were enucleated at the end of the reperfusion period. Biochemical assay and histopathologic evaluation was performed on one randomly selected eye of each animal. The level of retinal-free malondialdehyde (MDA) and retinal layer thicknesses were determined and comparisons were then made with the control group.

Results: The mean free MDA level increased in the placebo group (P = 0.006) but not in the drug group (P > 0.05). We observed polymorphonucleated leukocyte infiltration, retinal edema and hydropic degeneration in the retina of the placebo group. However, significant histopathologic change was not observed in specimens of the drug group.

Conclusions: This study suggests TMZ has a beneficial effect on retinal lipid peroxidation and histopathologic changes due to I/R injury.

No MeSH data available.


Related in: MedlinePlus