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Serum levels of soluble Fas, soluble tumor necrosis factor-receptor II, interleukin-2 receptor and interleukin-8 as early predictors of hepatocellular carcinoma in Egyptian patients with hepatitis C virus genotype-4.

Zekri AR, Alam El-Din HM, Bahnassy AA, Zayed NA, Mohamed WS, El-Masry SH, Gouda SK, Esmat G - Comp Hepatol (2010)

Bottom Line: HCC patients had higher levels of liver enzymes but lower log-HCV titer when compared to the other groups.HCC patients had also significantly higher levels of sFas, sTNFR-II and IL-2R and significantly lower levels of IL-8 when compared to the other groups.Exclusion of HCC among patients having PNALT could be predicted with 90 % sensitivity and 70.6 % specificity when sTNFR-II is [greater than or equal to] 389 pg/ml or IL-8 is < 290 pg/ml.

View Article: PubMed Central - HTML - PubMed

ABSTRACT

Background: Liver disease progression from chronic hepatitis C virus (HCV) infection to hepatocellular carcinoma (HCC) is associated with an imbalance between T-helper 1 and T-helper 2 cytokines. Evaluation of cytokines as possible candidate biomarkers for prediction of HCC was performed using soluble Fas(sFas), soluble tumor necrosis factor receptor-II (sTNFR-II), interleukin-2 receptor (IL-2R) and interleukin-8 (IL-8).

Results: The following patients were recruited: 79 with HCV infection, 30 with HCC, 32 with chronic liver disease associated with elevated liver enzyme levels (with or without cirrhosis) in addition to 17 with chronic HCV with persistent normal alanine aminotransferase levels (PNALT). Nine normal persons negative either for HCV or for hepatitis B virus were included as a control group. All persons were tested for sFas, sTNFR-II, IL-2R and IL-8 in their serum by quantitative ELISA. HCC patients had higher levels of liver enzymes but lower log-HCV titer when compared to the other groups. HCC patients had also significantly higher levels of sFas, sTNFR-II and IL-2R and significantly lower levels of IL-8 when compared to the other groups. Exclusion of HCC among patients having PNALT could be predicted with 90 % sensitivity and 70.6 % specificity when sTNFR-II is [greater than or equal to] 389 pg/ml or IL-8 is < 290 pg/ml.

Conclusions: Serum TNFR-II, IL-2Ralpha and IL-8, may be used as combined markers in HCV-infected cases for patients at high risk of developing HCC; further studies, however, are mandatory to check these findings before their application at the population level.

No MeSH data available.


Related in: MedlinePlus

Scatter diagram representing the distribution values of sFas in the different study groups. NC: normal controls; PNALT: Chronic hepatitis C with persistent normal alanine aminotrasferase; CLD: Chronic liver disease; HCC: hepatocellular carcinoma.
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Figure 2: Scatter diagram representing the distribution values of sFas in the different study groups. NC: normal controls; PNALT: Chronic hepatitis C with persistent normal alanine aminotrasferase; CLD: Chronic liver disease; HCC: hepatocellular carcinoma.

Mentions: Table 2 depicts the comparison of the serum levels of sFas, sTNFR-II, sIL-2Rα and IL-8. HCC patients had higher sFas, sTNFR-II and sIL-2R than patients with PNALT, CLD and normal controls with a significant difference for sFas between HCC patients and control (p < 0.001). The sTNFR-II was significantly elevated in HCC patients compared to those with PNALT and CLD (p < 0.001), whereas sIL-2R was significantly elevated in HCC patients when compared to those with PNALT patients and control. On the other hand, IL-8 was significantly lower among HCC patients when compared to the other groups (p < 0.001); but with no significance between the other groups. The scatter diagrams of the studied cytokines in the different study groups are shown in Figures 2, 3, 4 and 5.


Serum levels of soluble Fas, soluble tumor necrosis factor-receptor II, interleukin-2 receptor and interleukin-8 as early predictors of hepatocellular carcinoma in Egyptian patients with hepatitis C virus genotype-4.

Zekri AR, Alam El-Din HM, Bahnassy AA, Zayed NA, Mohamed WS, El-Masry SH, Gouda SK, Esmat G - Comp Hepatol (2010)

Scatter diagram representing the distribution values of sFas in the different study groups. NC: normal controls; PNALT: Chronic hepatitis C with persistent normal alanine aminotrasferase; CLD: Chronic liver disease; HCC: hepatocellular carcinoma.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2819041&req=5

Figure 2: Scatter diagram representing the distribution values of sFas in the different study groups. NC: normal controls; PNALT: Chronic hepatitis C with persistent normal alanine aminotrasferase; CLD: Chronic liver disease; HCC: hepatocellular carcinoma.
Mentions: Table 2 depicts the comparison of the serum levels of sFas, sTNFR-II, sIL-2Rα and IL-8. HCC patients had higher sFas, sTNFR-II and sIL-2R than patients with PNALT, CLD and normal controls with a significant difference for sFas between HCC patients and control (p < 0.001). The sTNFR-II was significantly elevated in HCC patients compared to those with PNALT and CLD (p < 0.001), whereas sIL-2R was significantly elevated in HCC patients when compared to those with PNALT patients and control. On the other hand, IL-8 was significantly lower among HCC patients when compared to the other groups (p < 0.001); but with no significance between the other groups. The scatter diagrams of the studied cytokines in the different study groups are shown in Figures 2, 3, 4 and 5.

Bottom Line: HCC patients had higher levels of liver enzymes but lower log-HCV titer when compared to the other groups.HCC patients had also significantly higher levels of sFas, sTNFR-II and IL-2R and significantly lower levels of IL-8 when compared to the other groups.Exclusion of HCC among patients having PNALT could be predicted with 90 % sensitivity and 70.6 % specificity when sTNFR-II is [greater than or equal to] 389 pg/ml or IL-8 is < 290 pg/ml.

View Article: PubMed Central - HTML - PubMed

ABSTRACT

Background: Liver disease progression from chronic hepatitis C virus (HCV) infection to hepatocellular carcinoma (HCC) is associated with an imbalance between T-helper 1 and T-helper 2 cytokines. Evaluation of cytokines as possible candidate biomarkers for prediction of HCC was performed using soluble Fas(sFas), soluble tumor necrosis factor receptor-II (sTNFR-II), interleukin-2 receptor (IL-2R) and interleukin-8 (IL-8).

Results: The following patients were recruited: 79 with HCV infection, 30 with HCC, 32 with chronic liver disease associated with elevated liver enzyme levels (with or without cirrhosis) in addition to 17 with chronic HCV with persistent normal alanine aminotransferase levels (PNALT). Nine normal persons negative either for HCV or for hepatitis B virus were included as a control group. All persons were tested for sFas, sTNFR-II, IL-2R and IL-8 in their serum by quantitative ELISA. HCC patients had higher levels of liver enzymes but lower log-HCV titer when compared to the other groups. HCC patients had also significantly higher levels of sFas, sTNFR-II and IL-2R and significantly lower levels of IL-8 when compared to the other groups. Exclusion of HCC among patients having PNALT could be predicted with 90 % sensitivity and 70.6 % specificity when sTNFR-II is [greater than or equal to] 389 pg/ml or IL-8 is < 290 pg/ml.

Conclusions: Serum TNFR-II, IL-2Ralpha and IL-8, may be used as combined markers in HCV-infected cases for patients at high risk of developing HCC; further studies, however, are mandatory to check these findings before their application at the population level.

No MeSH data available.


Related in: MedlinePlus