Limits...
The Locust Standard Brain: A 3D Standard of the Central Complex as a Platform for Neural Network Analysis.

El Jundi B, Heinze S, Lenschow C, Kurylas A, Rohlfing T, Homberg U - Front Syst Neurosci (2010)

Bottom Line: To explore the usefulness of this atlas, two central-complex neurons, a polarization-sensitive columnar neuron (type CPU1a) and a tangential neuron that is activated during flight, the giant fan-shaped (GFS) neuron, were reconstructed 3D from brain sections.To examine whether the GFS neuron is a candidate to contribute to synaptic input to the CPU1a neuron, we registered both neurons into the standardized central complex.Visualization of both neurons revealed a potential connection of the CPU1a and GFS neurons in layer II of the upper division of the central body.

View Article: PubMed Central - PubMed

Affiliation: Fachbereich Biologie, Tierphysiologie, Philipps-Universität Marburg Marburg, Germany.

ABSTRACT
Many insects use the pattern of polarized light in the sky for spatial orientation and navigation. We have investigated the polarization vision system in the desert locust. To create a common platform for anatomical studies on polarization vision pathways, Kurylas et al. (2008) have generated a three-dimensional (3D) standard brain from confocal microscopy image stacks of 10 male brains, using two different standardization methods, the Iterative Shape Averaging (ISA) procedure and the Virtual Insect Brain (VIB) protocol. Comparison of both standardization methods showed that the VIB standard is ideal for comparative volume analysis of neuropils, whereas the ISA standard is the method of choice to analyze the morphology and connectivity of neurons. The central complex is a key processing stage for polarization information in the locust brain. To investigate neuronal connections between diverse central-complex neurons, we generated a higher-resolution standard atlas of the central complex and surrounding areas, using the ISA method based on brain sections from 20 individual central complexes. To explore the usefulness of this atlas, two central-complex neurons, a polarization-sensitive columnar neuron (type CPU1a) and a tangential neuron that is activated during flight, the giant fan-shaped (GFS) neuron, were reconstructed 3D from brain sections. To examine whether the GFS neuron is a candidate to contribute to synaptic input to the CPU1a neuron, we registered both neurons into the standardized central complex. Visualization of both neurons revealed a potential connection of the CPU1a and GFS neurons in layer II of the upper division of the central body.

No MeSH data available.


Morphology of a CPU1a neuron. (A) Anterior view of a 3D volume rendering visualization of the CPU1a neuron and reconstruction of several neuropils of the central complex and lateral accessory lobe. CBL, lower division of the central body; CBU, upper division of the central body; LAL, lateral accessory lobe; PB, protocerebral bridge. Boxes show areas enlarged in B–D. (B) Location of cell body and arborizations in column R7 of the PB; maximum intensity projection of a confocal image stack. (C) Maximum intensity projections of ramifications of the CPU1 cell in two columns of the CBU, viewed from anteriorly. (D) Maximum intensity projection of optical slices of the arborizations in the ventral shell of the left LAL shown in anterior view. (E) 3D reconstruction of the CPU1a neuron before (red) and after affine and elastic registration (grey). Scale bars: (A,E) 100 μm; (B,C) 20 μm; (D) 50 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC2818101&req=5

Figure 5: Morphology of a CPU1a neuron. (A) Anterior view of a 3D volume rendering visualization of the CPU1a neuron and reconstruction of several neuropils of the central complex and lateral accessory lobe. CBL, lower division of the central body; CBU, upper division of the central body; LAL, lateral accessory lobe; PB, protocerebral bridge. Boxes show areas enlarged in B–D. (B) Location of cell body and arborizations in column R7 of the PB; maximum intensity projection of a confocal image stack. (C) Maximum intensity projections of ramifications of the CPU1 cell in two columns of the CBU, viewed from anteriorly. (D) Maximum intensity projection of optical slices of the arborizations in the ventral shell of the left LAL shown in anterior view. (E) 3D reconstruction of the CPU1a neuron before (red) and after affine and elastic registration (grey). Scale bars: (A,E) 100 μm; (B,C) 20 μm; (D) 50 μm.

Mentions: Visual comparison of the 10-animal ISA and VIB whole standard brains of the desert locust Schistocerca gregaria. Surface reconstruction of 33 distinct neuropils plus a “midbrain neuropil” (MN, transparent) (A) ISA standard brain in anterior (top), dorsal (middle), and posterior (bottom) view (aCa, accessory calyx; AL, antennal lobe; ALo, anterior lobe of the lobula; aMe, accessory medulla; CBL, lower division of the central body; CBU, upper division of the central body; DLo, dorsal lobe of the lobula; DRMe, dorsal rim area of the medulla; ILo, inner lobe of the lobula; lAOTu, lower unit of the anterior optic tubercle; LH, lateral horn; Me, medulla; No, noduli; OLo, outer lobe of the lobula; PB, protocerebral bridge; pCa, primary calyx; Pe, pedunculus; uAOTu, upper unit of the anterior optic tubercle). (B) VIB standard brain viewed from anterior (top), dorsal (middle), and posterior (bottom) sides. Visualization of the “midbrain neuropil” is based on average image data (modified from Kurylas et al., 2008, Figures 5A–C, right panel). The color coding of the neuropils is consistent with Brandt et al. (2005). Scale bar: 600 μm.


The Locust Standard Brain: A 3D Standard of the Central Complex as a Platform for Neural Network Analysis.

El Jundi B, Heinze S, Lenschow C, Kurylas A, Rohlfing T, Homberg U - Front Syst Neurosci (2010)

Morphology of a CPU1a neuron. (A) Anterior view of a 3D volume rendering visualization of the CPU1a neuron and reconstruction of several neuropils of the central complex and lateral accessory lobe. CBL, lower division of the central body; CBU, upper division of the central body; LAL, lateral accessory lobe; PB, protocerebral bridge. Boxes show areas enlarged in B–D. (B) Location of cell body and arborizations in column R7 of the PB; maximum intensity projection of a confocal image stack. (C) Maximum intensity projections of ramifications of the CPU1 cell in two columns of the CBU, viewed from anteriorly. (D) Maximum intensity projection of optical slices of the arborizations in the ventral shell of the left LAL shown in anterior view. (E) 3D reconstruction of the CPU1a neuron before (red) and after affine and elastic registration (grey). Scale bars: (A,E) 100 μm; (B,C) 20 μm; (D) 50 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2818101&req=5

Figure 5: Morphology of a CPU1a neuron. (A) Anterior view of a 3D volume rendering visualization of the CPU1a neuron and reconstruction of several neuropils of the central complex and lateral accessory lobe. CBL, lower division of the central body; CBU, upper division of the central body; LAL, lateral accessory lobe; PB, protocerebral bridge. Boxes show areas enlarged in B–D. (B) Location of cell body and arborizations in column R7 of the PB; maximum intensity projection of a confocal image stack. (C) Maximum intensity projections of ramifications of the CPU1 cell in two columns of the CBU, viewed from anteriorly. (D) Maximum intensity projection of optical slices of the arborizations in the ventral shell of the left LAL shown in anterior view. (E) 3D reconstruction of the CPU1a neuron before (red) and after affine and elastic registration (grey). Scale bars: (A,E) 100 μm; (B,C) 20 μm; (D) 50 μm.
Mentions: Visual comparison of the 10-animal ISA and VIB whole standard brains of the desert locust Schistocerca gregaria. Surface reconstruction of 33 distinct neuropils plus a “midbrain neuropil” (MN, transparent) (A) ISA standard brain in anterior (top), dorsal (middle), and posterior (bottom) view (aCa, accessory calyx; AL, antennal lobe; ALo, anterior lobe of the lobula; aMe, accessory medulla; CBL, lower division of the central body; CBU, upper division of the central body; DLo, dorsal lobe of the lobula; DRMe, dorsal rim area of the medulla; ILo, inner lobe of the lobula; lAOTu, lower unit of the anterior optic tubercle; LH, lateral horn; Me, medulla; No, noduli; OLo, outer lobe of the lobula; PB, protocerebral bridge; pCa, primary calyx; Pe, pedunculus; uAOTu, upper unit of the anterior optic tubercle). (B) VIB standard brain viewed from anterior (top), dorsal (middle), and posterior (bottom) sides. Visualization of the “midbrain neuropil” is based on average image data (modified from Kurylas et al., 2008, Figures 5A–C, right panel). The color coding of the neuropils is consistent with Brandt et al. (2005). Scale bar: 600 μm.

Bottom Line: To explore the usefulness of this atlas, two central-complex neurons, a polarization-sensitive columnar neuron (type CPU1a) and a tangential neuron that is activated during flight, the giant fan-shaped (GFS) neuron, were reconstructed 3D from brain sections.To examine whether the GFS neuron is a candidate to contribute to synaptic input to the CPU1a neuron, we registered both neurons into the standardized central complex.Visualization of both neurons revealed a potential connection of the CPU1a and GFS neurons in layer II of the upper division of the central body.

View Article: PubMed Central - PubMed

Affiliation: Fachbereich Biologie, Tierphysiologie, Philipps-Universität Marburg Marburg, Germany.

ABSTRACT
Many insects use the pattern of polarized light in the sky for spatial orientation and navigation. We have investigated the polarization vision system in the desert locust. To create a common platform for anatomical studies on polarization vision pathways, Kurylas et al. (2008) have generated a three-dimensional (3D) standard brain from confocal microscopy image stacks of 10 male brains, using two different standardization methods, the Iterative Shape Averaging (ISA) procedure and the Virtual Insect Brain (VIB) protocol. Comparison of both standardization methods showed that the VIB standard is ideal for comparative volume analysis of neuropils, whereas the ISA standard is the method of choice to analyze the morphology and connectivity of neurons. The central complex is a key processing stage for polarization information in the locust brain. To investigate neuronal connections between diverse central-complex neurons, we generated a higher-resolution standard atlas of the central complex and surrounding areas, using the ISA method based on brain sections from 20 individual central complexes. To explore the usefulness of this atlas, two central-complex neurons, a polarization-sensitive columnar neuron (type CPU1a) and a tangential neuron that is activated during flight, the giant fan-shaped (GFS) neuron, were reconstructed 3D from brain sections. To examine whether the GFS neuron is a candidate to contribute to synaptic input to the CPU1a neuron, we registered both neurons into the standardized central complex. Visualization of both neurons revealed a potential connection of the CPU1a and GFS neurons in layer II of the upper division of the central body.

No MeSH data available.