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West Nile virus from blood donors, vertebrates, and mosquitoes, Puerto Rico, 2007.

Hunsperger EA, McElroy KL, Bessoff K, Colón C, Barrera R, Muñoz-Jordán JL - Emerging Infect. Dis. (2009)

Bottom Line: West Nile virus (WNV) was isolated from a human blood donor, a dead falcon, and mosquitoes in Puerto Rico in 2007.Phylogenetic analysis of the 4 isolates suggests a recent introduction of lineage I WNV that is closely related to WNV currently circulating in North America.

View Article: PubMed Central - PubMed

Affiliation: Centers for Disease Control and Prevention, San Juan, Puerto Rico 00920, USA. enh4@cdc.gov

ABSTRACT
West Nile virus (WNV) was isolated from a human blood donor, a dead falcon, and mosquitoes in Puerto Rico in 2007. Phylogenetic analysis of the 4 isolates suggests a recent introduction of lineage I WNV that is closely related to WNV currently circulating in North America.

Show MeSH

Related in: MedlinePlus

Identification of West Nile virus (WNV) in Puerto Rico. WNV premembrane-envelope maximum likelihood phylogenetic tree of WNV isolates collected from 1998 through 2007, demonstrating the relationship of viruses from Puerto Rico to other lineage I isolates. Viruses are labeled by place and year of isolation and GenBank accession no. FJ799714–FJ799717 (Fiji). Clade names are consistent with those used by Davis et al. (14). Numbers indicate the neighbor-joining bootstrap values for groups in the tree and are shown when >70. Scale bar indicates nucleotide substitutions per site.
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Figure 2: Identification of West Nile virus (WNV) in Puerto Rico. WNV premembrane-envelope maximum likelihood phylogenetic tree of WNV isolates collected from 1998 through 2007, demonstrating the relationship of viruses from Puerto Rico to other lineage I isolates. Viruses are labeled by place and year of isolation and GenBank accession no. FJ799714–FJ799717 (Fiji). Clade names are consistent with those used by Davis et al. (14). Numbers indicate the neighbor-joining bootstrap values for groups in the tree and are shown when >70. Scale bar indicates nucleotide substitutions per site.

Mentions: WNV isolates from the falcon, human blood donation, sentinel chicken (7), and a pool of Cx. nigripalpus mosquitoes were sequenced to assess their phylogenetic relationships (Figure 2). Viral RNA was extracted from first passage Vero cell supernatant using the QIAamp Viral RNA kit (QIAGEN, Valencia, CA, USA). RT-PCR was performed by using the OneStep RT-PCR kit (QIAGEN) with WNV-specific primers (9), and DNA bands were excised and purified using the QIAquick gel-extraction kit (QIAGEN). The 2,004-nt premembrane-envelope (prM-E) genes of both DNA strands were directly sequenced by using the BigDye Terminator v3.1 Cycle Sequencing kit on a 3130X Genetic Analyzer (Applied Biosystems, Foster City, CA, USA).


West Nile virus from blood donors, vertebrates, and mosquitoes, Puerto Rico, 2007.

Hunsperger EA, McElroy KL, Bessoff K, Colón C, Barrera R, Muñoz-Jordán JL - Emerging Infect. Dis. (2009)

Identification of West Nile virus (WNV) in Puerto Rico. WNV premembrane-envelope maximum likelihood phylogenetic tree of WNV isolates collected from 1998 through 2007, demonstrating the relationship of viruses from Puerto Rico to other lineage I isolates. Viruses are labeled by place and year of isolation and GenBank accession no. FJ799714–FJ799717 (Fiji). Clade names are consistent with those used by Davis et al. (14). Numbers indicate the neighbor-joining bootstrap values for groups in the tree and are shown when >70. Scale bar indicates nucleotide substitutions per site.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2815984&req=5

Figure 2: Identification of West Nile virus (WNV) in Puerto Rico. WNV premembrane-envelope maximum likelihood phylogenetic tree of WNV isolates collected from 1998 through 2007, demonstrating the relationship of viruses from Puerto Rico to other lineage I isolates. Viruses are labeled by place and year of isolation and GenBank accession no. FJ799714–FJ799717 (Fiji). Clade names are consistent with those used by Davis et al. (14). Numbers indicate the neighbor-joining bootstrap values for groups in the tree and are shown when >70. Scale bar indicates nucleotide substitutions per site.
Mentions: WNV isolates from the falcon, human blood donation, sentinel chicken (7), and a pool of Cx. nigripalpus mosquitoes were sequenced to assess their phylogenetic relationships (Figure 2). Viral RNA was extracted from first passage Vero cell supernatant using the QIAamp Viral RNA kit (QIAGEN, Valencia, CA, USA). RT-PCR was performed by using the OneStep RT-PCR kit (QIAGEN) with WNV-specific primers (9), and DNA bands were excised and purified using the QIAquick gel-extraction kit (QIAGEN). The 2,004-nt premembrane-envelope (prM-E) genes of both DNA strands were directly sequenced by using the BigDye Terminator v3.1 Cycle Sequencing kit on a 3130X Genetic Analyzer (Applied Biosystems, Foster City, CA, USA).

Bottom Line: West Nile virus (WNV) was isolated from a human blood donor, a dead falcon, and mosquitoes in Puerto Rico in 2007.Phylogenetic analysis of the 4 isolates suggests a recent introduction of lineage I WNV that is closely related to WNV currently circulating in North America.

View Article: PubMed Central - PubMed

Affiliation: Centers for Disease Control and Prevention, San Juan, Puerto Rico 00920, USA. enh4@cdc.gov

ABSTRACT
West Nile virus (WNV) was isolated from a human blood donor, a dead falcon, and mosquitoes in Puerto Rico in 2007. Phylogenetic analysis of the 4 isolates suggests a recent introduction of lineage I WNV that is closely related to WNV currently circulating in North America.

Show MeSH
Related in: MedlinePlus