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Molecular epidemiology of rabies in Southern People's Republic of China.

Tao XY, Tang Q, Li H, Mo ZJ, Zhang H, Wang DM, Zhang Q, Song M, Velasco-Villa A, Wu X, Rupprecht CE, Liang GD - Emerging Infect. Dis. (2009)

Bottom Line: In recent years, the number of human rabies cases in the People's Republic of China has increased during severe epidemics in 3 southern provinces (Guizhou, Guangxi, and Hunan).Partial nucleoprotein gene sequences were obtained from rabies-positive specimens.Phylogenetic relationships and distribution of viruses were determined.

View Article: PubMed Central - PubMed

Affiliation: Chinese Center for Disease Control and Prevention, Beijing, People's Republic of China.

ABSTRACT
In recent years, the number of human rabies cases in the People's Republic of China has increased during severe epidemics in 3 southern provinces (Guizhou, Guangxi, and Hunan). To analyze the causes of the high incidence of human rabies in this region, during 2005-2007, we collected 2,887 brain specimens from apparently healthy domestic dogs used for meat consumption in restaurants, 4 specimens from suspected rabid dogs, and 3 from humans with rabies in the 3 provinces. Partial nucleoprotein gene sequences were obtained from rabies-positive specimens. Phylogenetic relationships and distribution of viruses were determined. We infer that the spread of rabies viruses from high-incidence regions, particularly by long-distance movement or transprovincial translocation of dogs caused by human-related activities, may be 1 cause of the recent massive human rabies epidemics in southern China.

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Related in: MedlinePlus

Neighbor-joining phylogenetic tree of 60 specimens of rabies virus (RABV) from the People’s Republic of China, 2005–2007, and different genotypes (GTs) from other areas based on a 720-nt (nt 704–nt 1423) nucleoprotein (N) gene fragment of RABV. Numbers at each node indicate degree of bootstrap support; only those with support >70% are indicated. Scale bar indicates nucleotide substitutions per site.
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Figure 3: Neighbor-joining phylogenetic tree of 60 specimens of rabies virus (RABV) from the People’s Republic of China, 2005–2007, and different genotypes (GTs) from other areas based on a 720-nt (nt 704–nt 1423) nucleoprotein (N) gene fragment of RABV. Numbers at each node indicate degree of bootstrap support; only those with support >70% are indicated. Scale bar indicates nucleotide substitutions per site.

Mentions: Most lineages and sublineages in the 60 samples from southern China were 100% identical (Figure 2). Thus, we chose representative samples to conduct a comparative phylogenetic analysis with RABV sequences from other countries and reservoir hosts (Figure 3). All virus specimens from southern China clustered in genotype 1 (GT1) and constituted a major branch (Asian dog; Figure 3) that is clearly segregated from other lyssavirus genotypes (GTs 2–7).


Molecular epidemiology of rabies in Southern People's Republic of China.

Tao XY, Tang Q, Li H, Mo ZJ, Zhang H, Wang DM, Zhang Q, Song M, Velasco-Villa A, Wu X, Rupprecht CE, Liang GD - Emerging Infect. Dis. (2009)

Neighbor-joining phylogenetic tree of 60 specimens of rabies virus (RABV) from the People’s Republic of China, 2005–2007, and different genotypes (GTs) from other areas based on a 720-nt (nt 704–nt 1423) nucleoprotein (N) gene fragment of RABV. Numbers at each node indicate degree of bootstrap support; only those with support >70% are indicated. Scale bar indicates nucleotide substitutions per site.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2815963&req=5

Figure 3: Neighbor-joining phylogenetic tree of 60 specimens of rabies virus (RABV) from the People’s Republic of China, 2005–2007, and different genotypes (GTs) from other areas based on a 720-nt (nt 704–nt 1423) nucleoprotein (N) gene fragment of RABV. Numbers at each node indicate degree of bootstrap support; only those with support >70% are indicated. Scale bar indicates nucleotide substitutions per site.
Mentions: Most lineages and sublineages in the 60 samples from southern China were 100% identical (Figure 2). Thus, we chose representative samples to conduct a comparative phylogenetic analysis with RABV sequences from other countries and reservoir hosts (Figure 3). All virus specimens from southern China clustered in genotype 1 (GT1) and constituted a major branch (Asian dog; Figure 3) that is clearly segregated from other lyssavirus genotypes (GTs 2–7).

Bottom Line: In recent years, the number of human rabies cases in the People's Republic of China has increased during severe epidemics in 3 southern provinces (Guizhou, Guangxi, and Hunan).Partial nucleoprotein gene sequences were obtained from rabies-positive specimens.Phylogenetic relationships and distribution of viruses were determined.

View Article: PubMed Central - PubMed

Affiliation: Chinese Center for Disease Control and Prevention, Beijing, People's Republic of China.

ABSTRACT
In recent years, the number of human rabies cases in the People's Republic of China has increased during severe epidemics in 3 southern provinces (Guizhou, Guangxi, and Hunan). To analyze the causes of the high incidence of human rabies in this region, during 2005-2007, we collected 2,887 brain specimens from apparently healthy domestic dogs used for meat consumption in restaurants, 4 specimens from suspected rabid dogs, and 3 from humans with rabies in the 3 provinces. Partial nucleoprotein gene sequences were obtained from rabies-positive specimens. Phylogenetic relationships and distribution of viruses were determined. We infer that the spread of rabies viruses from high-incidence regions, particularly by long-distance movement or transprovincial translocation of dogs caused by human-related activities, may be 1 cause of the recent massive human rabies epidemics in southern China.

Show MeSH
Related in: MedlinePlus