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Molecular epidemiology of rabies in Southern People's Republic of China.

Tao XY, Tang Q, Li H, Mo ZJ, Zhang H, Wang DM, Zhang Q, Song M, Velasco-Villa A, Wu X, Rupprecht CE, Liang GD - Emerging Infect. Dis. (2009)

Bottom Line: In recent years, the number of human rabies cases in the People's Republic of China has increased during severe epidemics in 3 southern provinces (Guizhou, Guangxi, and Hunan).Partial nucleoprotein gene sequences were obtained from rabies-positive specimens.Phylogenetic relationships and distribution of viruses were determined.

View Article: PubMed Central - PubMed

Affiliation: Chinese Center for Disease Control and Prevention, Beijing, People's Republic of China.

ABSTRACT
In recent years, the number of human rabies cases in the People's Republic of China has increased during severe epidemics in 3 southern provinces (Guizhou, Guangxi, and Hunan). To analyze the causes of the high incidence of human rabies in this region, during 2005-2007, we collected 2,887 brain specimens from apparently healthy domestic dogs used for meat consumption in restaurants, 4 specimens from suspected rabid dogs, and 3 from humans with rabies in the 3 provinces. Partial nucleoprotein gene sequences were obtained from rabies-positive specimens. Phylogenetic relationships and distribution of viruses were determined. We infer that the spread of rabies viruses from high-incidence regions, particularly by long-distance movement or transprovincial translocation of dogs caused by human-related activities, may be 1 cause of the recent massive human rabies epidemics in southern China.

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Related in: MedlinePlus

Neighbor-joining phylogenetic tree of 60 specimens of rabies virus from the People’s Republic of China, 2005–2007, based on a 720-nt (nt 704–nt 1423) nucleoprotein (N) gene fragment of rabies virus rooted with the Pasteur strain of rabies virus (PV). Numbers at each node indicate degree of bootstrap support; only those with support >70% are indicated. Taxa are from Hunan Province are shown in red, taxa are from Guangxi Province in black, and taxa are from Guizhou Province in blue. City, year of sample collection, and GenBank accession no. are included for each taxon. Stars indicate human samples; others are dog samples. YZ, Yongzhou; XX, Xiangxi; SY, Shaoyang; NN, Nanning; GG, Guigang; CD, Changde; XT, Xiangtan; LB, Laibin; LZ, Liuzhou; YL, Yulin; HC, Hechi; QX, Qianxinan; AS, Anshun. Scale bar indicates nucleotide substitutions per site.
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Figure 2: Neighbor-joining phylogenetic tree of 60 specimens of rabies virus from the People’s Republic of China, 2005–2007, based on a 720-nt (nt 704–nt 1423) nucleoprotein (N) gene fragment of rabies virus rooted with the Pasteur strain of rabies virus (PV). Numbers at each node indicate degree of bootstrap support; only those with support >70% are indicated. Taxa are from Hunan Province are shown in red, taxa are from Guangxi Province in black, and taxa are from Guizhou Province in blue. City, year of sample collection, and GenBank accession no. are included for each taxon. Stars indicate human samples; others are dog samples. YZ, Yongzhou; XX, Xiangxi; SY, Shaoyang; NN, Nanning; GG, Guigang; CD, Changde; XT, Xiangtan; LB, Laibin; LZ, Liuzhou; YL, Yulin; HC, Hechi; QX, Qianxinan; AS, Anshun. Scale bar indicates nucleotide substitutions per site.

Mentions: Results of a phylogenetic analysis of 60 sequences obtained in this study are shown in Figure 2; the Pasteur strain of RABV was used as an outgroup. The 60 virus specimens clustered into 3 statistically supported branches (bootstrap support 99%) and were designated as groups I, II, and III. Groups I and II can be further divided into 3 subgroups. Subgroup Ia contained specimens from Hunan Province (n = 9) and Guangxi Province (n = 6). All specimens in subgroup Ib were from Hunan Province. Most (7/11) specimens in subgroup Ic were from Guangxi Province, with 4 from Guizhou Province. The 3 specimens in subgroup IIa were from Hunan Province. Six of 7 specimens in subgroup IIb were from Guangxi Province; the other specimen was from Hunan Province. Subgroup IIc contained 4 specimens from Guangxi Province and 4 from Guizhou Province. Most (6/7) specimens in group III were from Guizhou Province; the other specimen was from Guangxi Province (Figure 1).


Molecular epidemiology of rabies in Southern People's Republic of China.

Tao XY, Tang Q, Li H, Mo ZJ, Zhang H, Wang DM, Zhang Q, Song M, Velasco-Villa A, Wu X, Rupprecht CE, Liang GD - Emerging Infect. Dis. (2009)

Neighbor-joining phylogenetic tree of 60 specimens of rabies virus from the People’s Republic of China, 2005–2007, based on a 720-nt (nt 704–nt 1423) nucleoprotein (N) gene fragment of rabies virus rooted with the Pasteur strain of rabies virus (PV). Numbers at each node indicate degree of bootstrap support; only those with support >70% are indicated. Taxa are from Hunan Province are shown in red, taxa are from Guangxi Province in black, and taxa are from Guizhou Province in blue. City, year of sample collection, and GenBank accession no. are included for each taxon. Stars indicate human samples; others are dog samples. YZ, Yongzhou; XX, Xiangxi; SY, Shaoyang; NN, Nanning; GG, Guigang; CD, Changde; XT, Xiangtan; LB, Laibin; LZ, Liuzhou; YL, Yulin; HC, Hechi; QX, Qianxinan; AS, Anshun. Scale bar indicates nucleotide substitutions per site.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2815963&req=5

Figure 2: Neighbor-joining phylogenetic tree of 60 specimens of rabies virus from the People’s Republic of China, 2005–2007, based on a 720-nt (nt 704–nt 1423) nucleoprotein (N) gene fragment of rabies virus rooted with the Pasteur strain of rabies virus (PV). Numbers at each node indicate degree of bootstrap support; only those with support >70% are indicated. Taxa are from Hunan Province are shown in red, taxa are from Guangxi Province in black, and taxa are from Guizhou Province in blue. City, year of sample collection, and GenBank accession no. are included for each taxon. Stars indicate human samples; others are dog samples. YZ, Yongzhou; XX, Xiangxi; SY, Shaoyang; NN, Nanning; GG, Guigang; CD, Changde; XT, Xiangtan; LB, Laibin; LZ, Liuzhou; YL, Yulin; HC, Hechi; QX, Qianxinan; AS, Anshun. Scale bar indicates nucleotide substitutions per site.
Mentions: Results of a phylogenetic analysis of 60 sequences obtained in this study are shown in Figure 2; the Pasteur strain of RABV was used as an outgroup. The 60 virus specimens clustered into 3 statistically supported branches (bootstrap support 99%) and were designated as groups I, II, and III. Groups I and II can be further divided into 3 subgroups. Subgroup Ia contained specimens from Hunan Province (n = 9) and Guangxi Province (n = 6). All specimens in subgroup Ib were from Hunan Province. Most (7/11) specimens in subgroup Ic were from Guangxi Province, with 4 from Guizhou Province. The 3 specimens in subgroup IIa were from Hunan Province. Six of 7 specimens in subgroup IIb were from Guangxi Province; the other specimen was from Hunan Province. Subgroup IIc contained 4 specimens from Guangxi Province and 4 from Guizhou Province. Most (6/7) specimens in group III were from Guizhou Province; the other specimen was from Guangxi Province (Figure 1).

Bottom Line: In recent years, the number of human rabies cases in the People's Republic of China has increased during severe epidemics in 3 southern provinces (Guizhou, Guangxi, and Hunan).Partial nucleoprotein gene sequences were obtained from rabies-positive specimens.Phylogenetic relationships and distribution of viruses were determined.

View Article: PubMed Central - PubMed

Affiliation: Chinese Center for Disease Control and Prevention, Beijing, People's Republic of China.

ABSTRACT
In recent years, the number of human rabies cases in the People's Republic of China has increased during severe epidemics in 3 southern provinces (Guizhou, Guangxi, and Hunan). To analyze the causes of the high incidence of human rabies in this region, during 2005-2007, we collected 2,887 brain specimens from apparently healthy domestic dogs used for meat consumption in restaurants, 4 specimens from suspected rabid dogs, and 3 from humans with rabies in the 3 provinces. Partial nucleoprotein gene sequences were obtained from rabies-positive specimens. Phylogenetic relationships and distribution of viruses were determined. We infer that the spread of rabies viruses from high-incidence regions, particularly by long-distance movement or transprovincial translocation of dogs caused by human-related activities, may be 1 cause of the recent massive human rabies epidemics in southern China.

Show MeSH
Related in: MedlinePlus