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Polymorphisms in gene encoding TRPV1-receptor involved in pain perception are unrelated to chronic pancreatitis.

van Esch AA, Lamberts MP, te Morsche RH, van Oijen MG, Jansen JB, Drenth JP - BMC Gastroenterol (2009)

Bottom Line: We generated 27 diplotypes and compared prevalence between patients and controls.Distribution of diplotypes was not statistically significantly different between patients and controls.TRPV1 diplotypes are not associated with chronic pancreatitis.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Gastroenterology & Hepatology, Radboud University Nijmegen Medical Centre, The Netherlands. A.vanEsch@MDL.umcn.nl

ABSTRACT

Background: The major clinical feature in chronic pancreatitis is pain, but the genetic basis of pancreatic pain in chronic pancreatitis is poorly understood. The transient receptor potential vanilloid receptor 1 (TRPV1) gene has been associated with pain perception, and genetic variations in TRPV1 may modify the presence and phenotype of chronic pancreatitis. The aim of our study was to investigate the genetic variation of TRPV1 in Dutch patients with chronic pancreatitis and healthy controls.

Methods: We genotyped 4 SNPs (rs222749, rs222747, rs224534 and rs8065080) in 228 chronic pancreatitis-patients and 207 healthy controls by PCR, followed by restriction-fragment-length-polymorphism analysis and DNA sequencing. We generated 27 diplotypes and compared prevalence between patients and controls.

Results: There was no significant difference in allele frequency of the 4 TRPV1 gene SNPs in patients with chronic pancreatitis and healthy controls. Distribution of diplotypes was not statistically significantly different between patients and controls.

Conclusion: TRPV1 diplotypes are not associated with chronic pancreatitis.

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Related in: MedlinePlus

Diplotype distribution of chronic pancreatitis patients and healthy controls. Distribution of diplotypes in patients with chronic pancreatitis and healthy controls. The diplotypes are compared to the most prevalent diplotype CCCA/CCCA (reference).
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Figure 2: Diplotype distribution of chronic pancreatitis patients and healthy controls. Distribution of diplotypes in patients with chronic pancreatitis and healthy controls. The diplotypes are compared to the most prevalent diplotype CCCA/CCCA (reference).

Mentions: Based on the SNP distribution, 27 diplotypes (Additional file 2 and Additional file 3) were generated which were present in chronic pancreatitis patients and in controls, of which 17 diplotypes were present in both groups. Diplotype CCCA/CCCA is most prevalent in both groups. This was therefore considered as the reference in calculating the odds ratios for the remaining diplotypes. Distribution of diplotypes was not statistically significantly different between patients and controls (Figure 2 and 3).


Polymorphisms in gene encoding TRPV1-receptor involved in pain perception are unrelated to chronic pancreatitis.

van Esch AA, Lamberts MP, te Morsche RH, van Oijen MG, Jansen JB, Drenth JP - BMC Gastroenterol (2009)

Diplotype distribution of chronic pancreatitis patients and healthy controls. Distribution of diplotypes in patients with chronic pancreatitis and healthy controls. The diplotypes are compared to the most prevalent diplotype CCCA/CCCA (reference).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2813232&req=5

Figure 2: Diplotype distribution of chronic pancreatitis patients and healthy controls. Distribution of diplotypes in patients with chronic pancreatitis and healthy controls. The diplotypes are compared to the most prevalent diplotype CCCA/CCCA (reference).
Mentions: Based on the SNP distribution, 27 diplotypes (Additional file 2 and Additional file 3) were generated which were present in chronic pancreatitis patients and in controls, of which 17 diplotypes were present in both groups. Diplotype CCCA/CCCA is most prevalent in both groups. This was therefore considered as the reference in calculating the odds ratios for the remaining diplotypes. Distribution of diplotypes was not statistically significantly different between patients and controls (Figure 2 and 3).

Bottom Line: We generated 27 diplotypes and compared prevalence between patients and controls.Distribution of diplotypes was not statistically significantly different between patients and controls.TRPV1 diplotypes are not associated with chronic pancreatitis.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Gastroenterology & Hepatology, Radboud University Nijmegen Medical Centre, The Netherlands. A.vanEsch@MDL.umcn.nl

ABSTRACT

Background: The major clinical feature in chronic pancreatitis is pain, but the genetic basis of pancreatic pain in chronic pancreatitis is poorly understood. The transient receptor potential vanilloid receptor 1 (TRPV1) gene has been associated with pain perception, and genetic variations in TRPV1 may modify the presence and phenotype of chronic pancreatitis. The aim of our study was to investigate the genetic variation of TRPV1 in Dutch patients with chronic pancreatitis and healthy controls.

Methods: We genotyped 4 SNPs (rs222749, rs222747, rs224534 and rs8065080) in 228 chronic pancreatitis-patients and 207 healthy controls by PCR, followed by restriction-fragment-length-polymorphism analysis and DNA sequencing. We generated 27 diplotypes and compared prevalence between patients and controls.

Results: There was no significant difference in allele frequency of the 4 TRPV1 gene SNPs in patients with chronic pancreatitis and healthy controls. Distribution of diplotypes was not statistically significantly different between patients and controls.

Conclusion: TRPV1 diplotypes are not associated with chronic pancreatitis.

Show MeSH
Related in: MedlinePlus