Limits...
Thickness of glomerular and tubular basement membranes in preclinical and clinical stages of diabetic nephropathy.

Tyagi I, Agrawal U, Amitabh V, Jain AK, Saxena S - Indian J Nephrol (2008)

Bottom Line: A significant increase was noted in the mean GBM and TBM thickness in both the preclinical and clinical groups compared to the control group.The changes in the TBM were noted to be predominant in both preclinical and clinical patients.This study indicates the importance of morphometric evaluation of the GBM and TBM width in the elucidation of early renal damage in diabetic nephropathy, especially in the absence of LM changes.

View Article: PubMed Central - PubMed

ABSTRACT

Aims: This study aimed to elucidate the early renal changes in diabetes mellitus (DM) with and without clinical symptoms related to renal damage.

Methods: Renal biopsy was studied in 25 patients (14 with microalbuminuria and 11 with albuminuria) both by light and electron microscopies (LM and EM, respectively) for renal changes and morphometry was performed to study glomerular and tubular basement membranes (GBM and TBM, respectively) width using a Soft Imaging System GmBH (analysis 3).

Results: A significant increase was noted in the mean GBM and TBM thickness in both the preclinical and clinical groups compared to the control group. The changes in the TBM were noted to be predominant in both preclinical and clinical patients.

Conclusions: This study indicates the importance of morphometric evaluation of the GBM and TBM width in the elucidation of early renal damage in diabetic nephropathy, especially in the absence of LM changes. The significance of identification of early renal changes using morphometric techniques for better management of these patients requires further studies.

No MeSH data available.


Related in: MedlinePlus

Ultrastructure of the glomerular capillary loop with a grid overlay for morphometric assessment
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC2813125&req=5

Figure 0001: Ultrastructure of the glomerular capillary loop with a grid overlay for morphometric assessment

Mentions: A series of images covering the entire available glomeruli and tubules were obtained through Gatan charge-coupled device (CCD) camera at a magnification of 6000x; these images were then converted to JPEG format to perform morphometric analysis to measure the GBM and TBM thickness by using a Soft Imaging System GmbH (analysis 3). The images were calibrated using an inbuilt micron bar. A 200 × 200 nm grid was overlaid on the images[Fig.1]. GBM and TBM width was obtained by the orthogonal intercept method. The measurements were made at each point where a grid line intercepted an endothelial/peripheral GBM interface. The GBM width was measured on a line orthogonal to the edge of the peripheral GBM at the endothelial side of the intercept. TBM was measured at 50 orthogonal intercepts per patient and presented as mean ± SD.


Thickness of glomerular and tubular basement membranes in preclinical and clinical stages of diabetic nephropathy.

Tyagi I, Agrawal U, Amitabh V, Jain AK, Saxena S - Indian J Nephrol (2008)

Ultrastructure of the glomerular capillary loop with a grid overlay for morphometric assessment
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2813125&req=5

Figure 0001: Ultrastructure of the glomerular capillary loop with a grid overlay for morphometric assessment
Mentions: A series of images covering the entire available glomeruli and tubules were obtained through Gatan charge-coupled device (CCD) camera at a magnification of 6000x; these images were then converted to JPEG format to perform morphometric analysis to measure the GBM and TBM thickness by using a Soft Imaging System GmbH (analysis 3). The images were calibrated using an inbuilt micron bar. A 200 × 200 nm grid was overlaid on the images[Fig.1]. GBM and TBM width was obtained by the orthogonal intercept method. The measurements were made at each point where a grid line intercepted an endothelial/peripheral GBM interface. The GBM width was measured on a line orthogonal to the edge of the peripheral GBM at the endothelial side of the intercept. TBM was measured at 50 orthogonal intercepts per patient and presented as mean ± SD.

Bottom Line: A significant increase was noted in the mean GBM and TBM thickness in both the preclinical and clinical groups compared to the control group.The changes in the TBM were noted to be predominant in both preclinical and clinical patients.This study indicates the importance of morphometric evaluation of the GBM and TBM width in the elucidation of early renal damage in diabetic nephropathy, especially in the absence of LM changes.

View Article: PubMed Central - PubMed

ABSTRACT

Aims: This study aimed to elucidate the early renal changes in diabetes mellitus (DM) with and without clinical symptoms related to renal damage.

Methods: Renal biopsy was studied in 25 patients (14 with microalbuminuria and 11 with albuminuria) both by light and electron microscopies (LM and EM, respectively) for renal changes and morphometry was performed to study glomerular and tubular basement membranes (GBM and TBM, respectively) width using a Soft Imaging System GmBH (analysis 3).

Results: A significant increase was noted in the mean GBM and TBM thickness in both the preclinical and clinical groups compared to the control group. The changes in the TBM were noted to be predominant in both preclinical and clinical patients.

Conclusions: This study indicates the importance of morphometric evaluation of the GBM and TBM width in the elucidation of early renal damage in diabetic nephropathy, especially in the absence of LM changes. The significance of identification of early renal changes using morphometric techniques for better management of these patients requires further studies.

No MeSH data available.


Related in: MedlinePlus