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Fiber optic backscatter spectroscopic sensor to monitor enamel demineralization and remineralization in vitro.

Kishen A, Shrestha A, Rafique A - J Conserv Dent (2008)

Bottom Line: A bifurcated fiber optic backscatter probe connected to a visible light source and a high resolution spectrophotometer was used to acquire the backscatter light spectrum from the tooth surface.The enamel and dentine specimens from the Part-1 experiments showed distinct backscatter spectra.The spectrum obtained from the enamel-dentine combination and the spectrum generated from the average of the enamel and dentine spectral values were closely similar and showed characteristics of dentine.

View Article: PubMed Central - PubMed

Affiliation: Department of Restorative Dentistry, Faculty of Dentistry, National University of Singapore, Republic of Singapore, Singapore.

ABSTRACT
In this study, a Fiber Optic Backscatter Spectroscopic Sensor (FOBSS) is used to monitor demineralization and remineralization induced changes in the enamel. A bifurcated fiber optic backscatter probe connected to a visible light source and a high resolution spectrophotometer was used to acquire the backscatter light spectrum from the tooth surface. The experiments were conducted in two parts. In Part 1, experiments were carried out using fiber optic backscatter spectroscopy on (1) sound enamel and dentine sections and (2) sound tooth specimens subjected to demineralization and remineralization. In Part 2, polarization microscopy was conducted to examine the depth of demineralization in tooth specimens. The enamel and dentine specimens from the Part-1 experiments showed distinct backscatter spectra. The spectrum obtained from the enamel-dentine combination and the spectrum generated from the average of the enamel and dentine spectral values were closely similar and showed characteristics of dentine. The experiments in Part 2 showed that demineralization and remineralization processes induced a linear decrease and linear increase in the backscatter light intensity respectively. A negative correlation between the decrease in the backscatter light intensity during demineralization and the depth of demineralization determined using the polarization microscopy was calculated to be p = -0.994. This in vitro experiment highlights the potential benefit of using FOBSS to detect demineralization and remineralization of enamel.

No MeSH data available.


Related in: MedlinePlus

The polarization microscopic images of the demineralized tooth specimens
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Figure 0007: The polarization microscopic images of the demineralized tooth specimens

Mentions: Figure 7 shows the polarization microscopic images of the demineralized tooth sections. The average depth of enamel demineralization was 56.21µm, after 24 hours of demineralization; it increased to 318µm after 96 hours of demineralization. There was a linear increase in the depth demineralization in the enamel, with the duration of demineralization [Figure 8]. There was a negative correlation between the increase in the depth of demineralization and the decrease in the backscatter light intensity, with duration of demineralization (P = −0.994).


Fiber optic backscatter spectroscopic sensor to monitor enamel demineralization and remineralization in vitro.

Kishen A, Shrestha A, Rafique A - J Conserv Dent (2008)

The polarization microscopic images of the demineralized tooth specimens
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2813093&req=5

Figure 0007: The polarization microscopic images of the demineralized tooth specimens
Mentions: Figure 7 shows the polarization microscopic images of the demineralized tooth sections. The average depth of enamel demineralization was 56.21µm, after 24 hours of demineralization; it increased to 318µm after 96 hours of demineralization. There was a linear increase in the depth demineralization in the enamel, with the duration of demineralization [Figure 8]. There was a negative correlation between the increase in the depth of demineralization and the decrease in the backscatter light intensity, with duration of demineralization (P = −0.994).

Bottom Line: A bifurcated fiber optic backscatter probe connected to a visible light source and a high resolution spectrophotometer was used to acquire the backscatter light spectrum from the tooth surface.The enamel and dentine specimens from the Part-1 experiments showed distinct backscatter spectra.The spectrum obtained from the enamel-dentine combination and the spectrum generated from the average of the enamel and dentine spectral values were closely similar and showed characteristics of dentine.

View Article: PubMed Central - PubMed

Affiliation: Department of Restorative Dentistry, Faculty of Dentistry, National University of Singapore, Republic of Singapore, Singapore.

ABSTRACT
In this study, a Fiber Optic Backscatter Spectroscopic Sensor (FOBSS) is used to monitor demineralization and remineralization induced changes in the enamel. A bifurcated fiber optic backscatter probe connected to a visible light source and a high resolution spectrophotometer was used to acquire the backscatter light spectrum from the tooth surface. The experiments were conducted in two parts. In Part 1, experiments were carried out using fiber optic backscatter spectroscopy on (1) sound enamel and dentine sections and (2) sound tooth specimens subjected to demineralization and remineralization. In Part 2, polarization microscopy was conducted to examine the depth of demineralization in tooth specimens. The enamel and dentine specimens from the Part-1 experiments showed distinct backscatter spectra. The spectrum obtained from the enamel-dentine combination and the spectrum generated from the average of the enamel and dentine spectral values were closely similar and showed characteristics of dentine. The experiments in Part 2 showed that demineralization and remineralization processes induced a linear decrease and linear increase in the backscatter light intensity respectively. A negative correlation between the decrease in the backscatter light intensity during demineralization and the depth of demineralization determined using the polarization microscopy was calculated to be p = -0.994. This in vitro experiment highlights the potential benefit of using FOBSS to detect demineralization and remineralization of enamel.

No MeSH data available.


Related in: MedlinePlus