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Fiber optic backscatter spectroscopic sensor to monitor enamel demineralization and remineralization in vitro.

Kishen A, Shrestha A, Rafique A - J Conserv Dent (2008)

Bottom Line: A bifurcated fiber optic backscatter probe connected to a visible light source and a high resolution spectrophotometer was used to acquire the backscatter light spectrum from the tooth surface.The enamel and dentine specimens from the Part-1 experiments showed distinct backscatter spectra.The spectrum obtained from the enamel-dentine combination and the spectrum generated from the average of the enamel and dentine spectral values were closely similar and showed characteristics of dentine.

View Article: PubMed Central - PubMed

Affiliation: Department of Restorative Dentistry, Faculty of Dentistry, National University of Singapore, Republic of Singapore, Singapore.

ABSTRACT
In this study, a Fiber Optic Backscatter Spectroscopic Sensor (FOBSS) is used to monitor demineralization and remineralization induced changes in the enamel. A bifurcated fiber optic backscatter probe connected to a visible light source and a high resolution spectrophotometer was used to acquire the backscatter light spectrum from the tooth surface. The experiments were conducted in two parts. In Part 1, experiments were carried out using fiber optic backscatter spectroscopy on (1) sound enamel and dentine sections and (2) sound tooth specimens subjected to demineralization and remineralization. In Part 2, polarization microscopy was conducted to examine the depth of demineralization in tooth specimens. The enamel and dentine specimens from the Part-1 experiments showed distinct backscatter spectra. The spectrum obtained from the enamel-dentine combination and the spectrum generated from the average of the enamel and dentine spectral values were closely similar and showed characteristics of dentine. The experiments in Part 2 showed that demineralization and remineralization processes induced a linear decrease and linear increase in the backscatter light intensity respectively. A negative correlation between the decrease in the backscatter light intensity during demineralization and the depth of demineralization determined using the polarization microscopy was calculated to be p = -0.994. This in vitro experiment highlights the potential benefit of using FOBSS to detect demineralization and remineralization of enamel.

No MeSH data available.


Related in: MedlinePlus

The graph showing the shift in the average normalized backscatter light intensity (at 670 nm), with different intervals of remineralization
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Figure 0006: The graph showing the shift in the average normalized backscatter light intensity (at 670 nm), with different intervals of remineralization

Mentions: Stage 2: Experiments on tooth specimens: The spectral pattern obtained from all undemineralized tooth surface was identical for all samples. Figure 5 shows the variation in the average normalized backscatter light intensity (at 670 nm), after different intervals of demineralization. It was observed that enamel demineralization produced a linear decrease in intensity (at 670nm), with increase in the duration of demineralization. Figure 6 shows the average normalized backscatter light intensity after different intervals of remineralization. It was found that remineralization produced an increase in the backscatter light intensity (at 670nm). There was a linear increase in light intensity, with increase in the duration of remineralization.


Fiber optic backscatter spectroscopic sensor to monitor enamel demineralization and remineralization in vitro.

Kishen A, Shrestha A, Rafique A - J Conserv Dent (2008)

The graph showing the shift in the average normalized backscatter light intensity (at 670 nm), with different intervals of remineralization
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2813093&req=5

Figure 0006: The graph showing the shift in the average normalized backscatter light intensity (at 670 nm), with different intervals of remineralization
Mentions: Stage 2: Experiments on tooth specimens: The spectral pattern obtained from all undemineralized tooth surface was identical for all samples. Figure 5 shows the variation in the average normalized backscatter light intensity (at 670 nm), after different intervals of demineralization. It was observed that enamel demineralization produced a linear decrease in intensity (at 670nm), with increase in the duration of demineralization. Figure 6 shows the average normalized backscatter light intensity after different intervals of remineralization. It was found that remineralization produced an increase in the backscatter light intensity (at 670nm). There was a linear increase in light intensity, with increase in the duration of remineralization.

Bottom Line: A bifurcated fiber optic backscatter probe connected to a visible light source and a high resolution spectrophotometer was used to acquire the backscatter light spectrum from the tooth surface.The enamel and dentine specimens from the Part-1 experiments showed distinct backscatter spectra.The spectrum obtained from the enamel-dentine combination and the spectrum generated from the average of the enamel and dentine spectral values were closely similar and showed characteristics of dentine.

View Article: PubMed Central - PubMed

Affiliation: Department of Restorative Dentistry, Faculty of Dentistry, National University of Singapore, Republic of Singapore, Singapore.

ABSTRACT
In this study, a Fiber Optic Backscatter Spectroscopic Sensor (FOBSS) is used to monitor demineralization and remineralization induced changes in the enamel. A bifurcated fiber optic backscatter probe connected to a visible light source and a high resolution spectrophotometer was used to acquire the backscatter light spectrum from the tooth surface. The experiments were conducted in two parts. In Part 1, experiments were carried out using fiber optic backscatter spectroscopy on (1) sound enamel and dentine sections and (2) sound tooth specimens subjected to demineralization and remineralization. In Part 2, polarization microscopy was conducted to examine the depth of demineralization in tooth specimens. The enamel and dentine specimens from the Part-1 experiments showed distinct backscatter spectra. The spectrum obtained from the enamel-dentine combination and the spectrum generated from the average of the enamel and dentine spectral values were closely similar and showed characteristics of dentine. The experiments in Part 2 showed that demineralization and remineralization processes induced a linear decrease and linear increase in the backscatter light intensity respectively. A negative correlation between the decrease in the backscatter light intensity during demineralization and the depth of demineralization determined using the polarization microscopy was calculated to be p = -0.994. This in vitro experiment highlights the potential benefit of using FOBSS to detect demineralization and remineralization of enamel.

No MeSH data available.


Related in: MedlinePlus