Limits...
Effect of Potato virus Y on the NADP-malic enzyme from Nicotiana tabacum L.: mRNA, expressed protein and activity.

Doubnerová V, Müller K, Cerovská N, Synková H, Spoustová P, Ryslavá H - Int J Mol Sci (2009)

Bottom Line: Petit Havana, SR1) was tested at the transcriptional, translational and activity level.The effect of viral infection induced by Potato virus Y, NTN strain, causing more severe symptoms, was compared with the effect induced by milder strain PVY(O).The relevance of NADP-malic enzyme in plants under stress conditions was discussed.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, Charles University, Prague, Czech Republic. doubnerova@volny.cz <doubnerova@volny.cz>

ABSTRACT
The effect of biotic stress induced by viral infection (Potato virus Y, strain NTN and O) on NADP-malic enzyme (EC 1.1.1.40) in tobacco plants (Nicotiana tabacum L., cv. Petit Havana, SR1) was tested at the transcriptional, translational and activity level. The increase of enzyme activity in infected leaves was correlated with the increased amount of expressed protein and with mRNA of cytosolic NADP-ME isoform. Transcription of the chloroplastic enzyme was not influenced by viral infection. The increase of the enzyme activity was also detected in stems and roots of infected plants. The effect of viral infection induced by Potato virus Y, NTN strain, causing more severe symptoms, was compared with the effect induced by milder strain PVY(O). The observed increase in NADP-malic enzyme activity in all parts of the studied plants was higher in the case of PVY(NTN) strain than in the case of strain PVY(O). The relevance of NADP-malic enzyme in plants under stress conditions was discussed.

Show MeSH

Related in: MedlinePlus

Concentrations of important cell metabolites. Measurement of concentrations of L-malate, D-glucose and D-glucose-6-phosphate in Nicotiana tabacum L. leaves of healthy plants and those infected by PVYNTN. Relative concentrations (expressed as % of healthy controls) are presented (L-malate: 100% corresponds to 10.5 mmol·g−1 F.W.; D-glucose: 100% corresponds to 2.8 mmol·g−1 F.W.; D-glucose-6-phosphate: 100% means 0.06 mmol·g−1 F.W.).Statistical analysis was done using t-test. * denotes significant difference from controls at P<0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC2812832&req=5

f7-ijms-10-03583: Concentrations of important cell metabolites. Measurement of concentrations of L-malate, D-glucose and D-glucose-6-phosphate in Nicotiana tabacum L. leaves of healthy plants and those infected by PVYNTN. Relative concentrations (expressed as % of healthy controls) are presented (L-malate: 100% corresponds to 10.5 mmol·g−1 F.W.; D-glucose: 100% corresponds to 2.8 mmol·g−1 F.W.; D-glucose-6-phosphate: 100% means 0.06 mmol·g−1 F.W.).Statistical analysis was done using t-test. * denotes significant difference from controls at P<0.05.

Mentions: Enhanced NADP-ME activity corresponded also to higher concentration of l-malate in infected plants (Figure 7). Another source of NADPH is the reaction catalyzed by d-glucose-6-phosphate dehydrogenase. Concentration of d-glucose-6-phosphate was enhanced in the maximum of PVYNTN symptom development (Figure 7). Whereas substrates of NADP-dependent enzymes (l-malate and d-glucose-6-phosphate) were approximately 2- and 4-fold, respectively higher in PVYNTN infected tobacco leaves (Figure 7); concentration of d-glucose was similar in infected and healthy plants.


Effect of Potato virus Y on the NADP-malic enzyme from Nicotiana tabacum L.: mRNA, expressed protein and activity.

Doubnerová V, Müller K, Cerovská N, Synková H, Spoustová P, Ryslavá H - Int J Mol Sci (2009)

Concentrations of important cell metabolites. Measurement of concentrations of L-malate, D-glucose and D-glucose-6-phosphate in Nicotiana tabacum L. leaves of healthy plants and those infected by PVYNTN. Relative concentrations (expressed as % of healthy controls) are presented (L-malate: 100% corresponds to 10.5 mmol·g−1 F.W.; D-glucose: 100% corresponds to 2.8 mmol·g−1 F.W.; D-glucose-6-phosphate: 100% means 0.06 mmol·g−1 F.W.).Statistical analysis was done using t-test. * denotes significant difference from controls at P<0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC2812832&req=5

f7-ijms-10-03583: Concentrations of important cell metabolites. Measurement of concentrations of L-malate, D-glucose and D-glucose-6-phosphate in Nicotiana tabacum L. leaves of healthy plants and those infected by PVYNTN. Relative concentrations (expressed as % of healthy controls) are presented (L-malate: 100% corresponds to 10.5 mmol·g−1 F.W.; D-glucose: 100% corresponds to 2.8 mmol·g−1 F.W.; D-glucose-6-phosphate: 100% means 0.06 mmol·g−1 F.W.).Statistical analysis was done using t-test. * denotes significant difference from controls at P<0.05.
Mentions: Enhanced NADP-ME activity corresponded also to higher concentration of l-malate in infected plants (Figure 7). Another source of NADPH is the reaction catalyzed by d-glucose-6-phosphate dehydrogenase. Concentration of d-glucose-6-phosphate was enhanced in the maximum of PVYNTN symptom development (Figure 7). Whereas substrates of NADP-dependent enzymes (l-malate and d-glucose-6-phosphate) were approximately 2- and 4-fold, respectively higher in PVYNTN infected tobacco leaves (Figure 7); concentration of d-glucose was similar in infected and healthy plants.

Bottom Line: Petit Havana, SR1) was tested at the transcriptional, translational and activity level.The effect of viral infection induced by Potato virus Y, NTN strain, causing more severe symptoms, was compared with the effect induced by milder strain PVY(O).The relevance of NADP-malic enzyme in plants under stress conditions was discussed.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, Charles University, Prague, Czech Republic. doubnerova@volny.cz <doubnerova@volny.cz>

ABSTRACT
The effect of biotic stress induced by viral infection (Potato virus Y, strain NTN and O) on NADP-malic enzyme (EC 1.1.1.40) in tobacco plants (Nicotiana tabacum L., cv. Petit Havana, SR1) was tested at the transcriptional, translational and activity level. The increase of enzyme activity in infected leaves was correlated with the increased amount of expressed protein and with mRNA of cytosolic NADP-ME isoform. Transcription of the chloroplastic enzyme was not influenced by viral infection. The increase of the enzyme activity was also detected in stems and roots of infected plants. The effect of viral infection induced by Potato virus Y, NTN strain, causing more severe symptoms, was compared with the effect induced by milder strain PVY(O). The observed increase in NADP-malic enzyme activity in all parts of the studied plants was higher in the case of PVY(NTN) strain than in the case of strain PVY(O). The relevance of NADP-malic enzyme in plants under stress conditions was discussed.

Show MeSH
Related in: MedlinePlus