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Effect of Potato virus Y on the NADP-malic enzyme from Nicotiana tabacum L.: mRNA, expressed protein and activity.

Doubnerová V, Müller K, Cerovská N, Synková H, Spoustová P, Ryslavá H - Int J Mol Sci (2009)

Bottom Line: Petit Havana, SR1) was tested at the transcriptional, translational and activity level.The effect of viral infection induced by Potato virus Y, NTN strain, causing more severe symptoms, was compared with the effect induced by milder strain PVY(O).The relevance of NADP-malic enzyme in plants under stress conditions was discussed.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, Charles University, Prague, Czech Republic. doubnerova@volny.cz <doubnerova@volny.cz>

ABSTRACT
The effect of biotic stress induced by viral infection (Potato virus Y, strain NTN and O) on NADP-malic enzyme (EC 1.1.1.40) in tobacco plants (Nicotiana tabacum L., cv. Petit Havana, SR1) was tested at the transcriptional, translational and activity level. The increase of enzyme activity in infected leaves was correlated with the increased amount of expressed protein and with mRNA of cytosolic NADP-ME isoform. Transcription of the chloroplastic enzyme was not influenced by viral infection. The increase of the enzyme activity was also detected in stems and roots of infected plants. The effect of viral infection induced by Potato virus Y, NTN strain, causing more severe symptoms, was compared with the effect induced by milder strain PVY(O). The observed increase in NADP-malic enzyme activity in all parts of the studied plants was higher in the case of PVY(NTN) strain than in the case of strain PVY(O). The relevance of NADP-malic enzyme in plants under stress conditions was discussed.

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Activities of NADP-ME from Nicotiana tabacum L. stems (a) and roots (b) collected 11th day after PVYO inoculation and 13th day after PVYNTN inoculation calculated per fresh mass. The activity of each sample is shown as percentage the activity value of the non-infected control, where 100% NADP-ME is in stems 0.040 ± 0.005 μmol·min−1·g−1, in roots 0.076 ± 0.016 μmol·min−1·g−1.The activity was measured in at least six samples, S.D. are shown. Statistical analysis was done using t-test. * denotes significant difference from controls at P<0.05.
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f3-ijms-10-03583: Activities of NADP-ME from Nicotiana tabacum L. stems (a) and roots (b) collected 11th day after PVYO inoculation and 13th day after PVYNTN inoculation calculated per fresh mass. The activity of each sample is shown as percentage the activity value of the non-infected control, where 100% NADP-ME is in stems 0.040 ± 0.005 μmol·min−1·g−1, in roots 0.076 ± 0.016 μmol·min−1·g−1.The activity was measured in at least six samples, S.D. are shown. Statistical analysis was done using t-test. * denotes significant difference from controls at P<0.05.

Mentions: NADP-ME activity in stems and roots (Figure 3) was measured in a period, when symptom development reached its maximum (i.e., 11th day of PVYO and 13th day of PVYNTN infection). NADP-ME activity in stems was 1.5-fold higher in plants infected by milder PVYO and 2.1-fold higher in plants infected by PVYNTN compared with control stems (Figure 3a). Similar situation was observed in roots: PVYO caused 1.4-fold increase in NADP-ME activity, whereas PVYNTN caused 2.4-fold increase in NADP-ME activity as compared with control roots (Figure 3b).


Effect of Potato virus Y on the NADP-malic enzyme from Nicotiana tabacum L.: mRNA, expressed protein and activity.

Doubnerová V, Müller K, Cerovská N, Synková H, Spoustová P, Ryslavá H - Int J Mol Sci (2009)

Activities of NADP-ME from Nicotiana tabacum L. stems (a) and roots (b) collected 11th day after PVYO inoculation and 13th day after PVYNTN inoculation calculated per fresh mass. The activity of each sample is shown as percentage the activity value of the non-infected control, where 100% NADP-ME is in stems 0.040 ± 0.005 μmol·min−1·g−1, in roots 0.076 ± 0.016 μmol·min−1·g−1.The activity was measured in at least six samples, S.D. are shown. Statistical analysis was done using t-test. * denotes significant difference from controls at P<0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC2812832&req=5

f3-ijms-10-03583: Activities of NADP-ME from Nicotiana tabacum L. stems (a) and roots (b) collected 11th day after PVYO inoculation and 13th day after PVYNTN inoculation calculated per fresh mass. The activity of each sample is shown as percentage the activity value of the non-infected control, where 100% NADP-ME is in stems 0.040 ± 0.005 μmol·min−1·g−1, in roots 0.076 ± 0.016 μmol·min−1·g−1.The activity was measured in at least six samples, S.D. are shown. Statistical analysis was done using t-test. * denotes significant difference from controls at P<0.05.
Mentions: NADP-ME activity in stems and roots (Figure 3) was measured in a period, when symptom development reached its maximum (i.e., 11th day of PVYO and 13th day of PVYNTN infection). NADP-ME activity in stems was 1.5-fold higher in plants infected by milder PVYO and 2.1-fold higher in plants infected by PVYNTN compared with control stems (Figure 3a). Similar situation was observed in roots: PVYO caused 1.4-fold increase in NADP-ME activity, whereas PVYNTN caused 2.4-fold increase in NADP-ME activity as compared with control roots (Figure 3b).

Bottom Line: Petit Havana, SR1) was tested at the transcriptional, translational and activity level.The effect of viral infection induced by Potato virus Y, NTN strain, causing more severe symptoms, was compared with the effect induced by milder strain PVY(O).The relevance of NADP-malic enzyme in plants under stress conditions was discussed.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, Charles University, Prague, Czech Republic. doubnerova@volny.cz <doubnerova@volny.cz>

ABSTRACT
The effect of biotic stress induced by viral infection (Potato virus Y, strain NTN and O) on NADP-malic enzyme (EC 1.1.1.40) in tobacco plants (Nicotiana tabacum L., cv. Petit Havana, SR1) was tested at the transcriptional, translational and activity level. The increase of enzyme activity in infected leaves was correlated with the increased amount of expressed protein and with mRNA of cytosolic NADP-ME isoform. Transcription of the chloroplastic enzyme was not influenced by viral infection. The increase of the enzyme activity was also detected in stems and roots of infected plants. The effect of viral infection induced by Potato virus Y, NTN strain, causing more severe symptoms, was compared with the effect induced by milder strain PVY(O). The observed increase in NADP-malic enzyme activity in all parts of the studied plants was higher in the case of PVY(NTN) strain than in the case of strain PVY(O). The relevance of NADP-malic enzyme in plants under stress conditions was discussed.

Show MeSH
Related in: MedlinePlus