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The role of bloom index of gelatin on the interaction with retinal pigment epithelial cells.

Lai JY - Int J Mol Sci (2009)

Bottom Line: This paper investigates the effects of the Bloom index of gelatin on its interaction with retinal pigment epithelial (RPE) cells.The mitochondrial dehydrogenase activity in the G300 groups was significantly lower than that of G75-100 and G175 groups.These findings suggest that the Bloom index gives influence on cellular responses to gelatin materials.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biochemical and Biomedical Engineering, Chang Gung University, Taoyuan, Taiwan. jylai@mail.cgu.edu.tw <jylai@mail.cgu.edu.tw>

ABSTRACT
Biocompatible materials are of considerable interest in the development of cell/drug delivery carriers for therapeutic applications. This paper investigates the effects of the Bloom index of gelatin on its interaction with retinal pigment epithelial (RPE) cells. Following two days of culture of ARPE-19 cells with gelatin samples G75-100, G175, and G300, the in vitro biocompatibility was determined by cell proliferation and viability assays, and glutamate uptake measurements, as well as cytokine expression analyses. The mitochondrial dehydrogenase activity in the G300 groups was significantly lower than that of G75-100 and G175 groups. The Live/Dead assays also showed that the gelatin samples G300 induced mild cytotoxicity. In comparison with the treatment of gelatins with low Bloom index, the exposure to high Bloom strength gelatins markedly reduced the glutamate uptake capacity of ARPE-19 cells. One possible explanation for these observations is that the presence of gelatin samples G300 with high viscosity in the medium may affect the nutrient availability to cultured cells. The analyses of pro-inflammatory cytokine IL-6 expression at both mRNA and protein levels showed that the gelatins with low Bloom index caused less cellular inflammatory reaction and had more acceptable biocompatibility than their high Bloom strength counterparts. These findings suggest that the Bloom index gives influence on cellular responses to gelatin materials.

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Level of IL-6 released from ARPE-19 cell cultures after incubation with various dissolved gelatin materials for two days.An asterisk indicates statistically significant differences (*p<0.05; n = 4) as compared to controls (without materials).
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f7-ijms-10-03442: Level of IL-6 released from ARPE-19 cell cultures after incubation with various dissolved gelatin materials for two days.An asterisk indicates statistically significant differences (*p<0.05; n = 4) as compared to controls (without materials).

Mentions: The ARPE-19 cell secretion of IL-6 in response to various gelatin membranes is also shown in Figure 7. The expression of IL-6 in the control, G75-100, and G175 groups was 187.4 ± 32.5, 206.1 ± 28.2, and 241.6 ± 24.3 pg/mL, respectively. The values did not show a significant difference between these three groups (p>0.05), which indicates that the exposure to gelatins with low Bloom index does not promote inflammation. However, the IL-6 level was significantly higher in the G300 groups (315.8 ± 26.1 pg/mL), compared with those of the low Bloom strength gelatin groups (p<0.05). These findings suggest that the Bloom index of gelatin has an important influence on the stimulation of pro-inflammatory cytokine IL-6 production in RPE cells.


The role of bloom index of gelatin on the interaction with retinal pigment epithelial cells.

Lai JY - Int J Mol Sci (2009)

Level of IL-6 released from ARPE-19 cell cultures after incubation with various dissolved gelatin materials for two days.An asterisk indicates statistically significant differences (*p<0.05; n = 4) as compared to controls (without materials).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC2812822&req=5

f7-ijms-10-03442: Level of IL-6 released from ARPE-19 cell cultures after incubation with various dissolved gelatin materials for two days.An asterisk indicates statistically significant differences (*p<0.05; n = 4) as compared to controls (without materials).
Mentions: The ARPE-19 cell secretion of IL-6 in response to various gelatin membranes is also shown in Figure 7. The expression of IL-6 in the control, G75-100, and G175 groups was 187.4 ± 32.5, 206.1 ± 28.2, and 241.6 ± 24.3 pg/mL, respectively. The values did not show a significant difference between these three groups (p>0.05), which indicates that the exposure to gelatins with low Bloom index does not promote inflammation. However, the IL-6 level was significantly higher in the G300 groups (315.8 ± 26.1 pg/mL), compared with those of the low Bloom strength gelatin groups (p<0.05). These findings suggest that the Bloom index of gelatin has an important influence on the stimulation of pro-inflammatory cytokine IL-6 production in RPE cells.

Bottom Line: This paper investigates the effects of the Bloom index of gelatin on its interaction with retinal pigment epithelial (RPE) cells.The mitochondrial dehydrogenase activity in the G300 groups was significantly lower than that of G75-100 and G175 groups.These findings suggest that the Bloom index gives influence on cellular responses to gelatin materials.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biochemical and Biomedical Engineering, Chang Gung University, Taoyuan, Taiwan. jylai@mail.cgu.edu.tw <jylai@mail.cgu.edu.tw>

ABSTRACT
Biocompatible materials are of considerable interest in the development of cell/drug delivery carriers for therapeutic applications. This paper investigates the effects of the Bloom index of gelatin on its interaction with retinal pigment epithelial (RPE) cells. Following two days of culture of ARPE-19 cells with gelatin samples G75-100, G175, and G300, the in vitro biocompatibility was determined by cell proliferation and viability assays, and glutamate uptake measurements, as well as cytokine expression analyses. The mitochondrial dehydrogenase activity in the G300 groups was significantly lower than that of G75-100 and G175 groups. The Live/Dead assays also showed that the gelatin samples G300 induced mild cytotoxicity. In comparison with the treatment of gelatins with low Bloom index, the exposure to high Bloom strength gelatins markedly reduced the glutamate uptake capacity of ARPE-19 cells. One possible explanation for these observations is that the presence of gelatin samples G300 with high viscosity in the medium may affect the nutrient availability to cultured cells. The analyses of pro-inflammatory cytokine IL-6 expression at both mRNA and protein levels showed that the gelatins with low Bloom index caused less cellular inflammatory reaction and had more acceptable biocompatibility than their high Bloom strength counterparts. These findings suggest that the Bloom index gives influence on cellular responses to gelatin materials.

Show MeSH
Related in: MedlinePlus